Publication Name	World Journal of Stem Cells
Manuscript ID	24267
Country	Puerto Rico

Received	2016-01-15 09:06
Peer-Review Started	2016-01-15 11:01
To Make the First Decision	2016-03-01 09:16
Return for Revision	2016-03-01 16:06
Revised	2016-07-21 00:00
Second Decision	2016-07-28 14:49
Accepted by Journal Editor-in-Chief	2016-07-28 16:36
Accepted by Executive Editor-in-Chief	2016-08-08 14:27
Articles in Press	2016-08-08 14:27
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2016-10-17 14:12
Publish the Manuscript Online	2016-10-20 21:54

ISSN	1948-0210 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Biochemistry & Molecular Biology
Manuscript Type	Basic Study
Article Title	Characterization and genetic manipulation of primed stem cells into a functional naïve state with ESRRB
Manuscript Source	Invited Manuscript
All Author List	Ricardo Antonio Rossello, Andreas Pfenning, Jason T Howard and Ute Hochgeschwender
Funding Agency and Grant Number	Funding Agency Grant Number Partially an NIH translational training T32NS051156 University of Puerto Rico Medical Sciences Campus 400100420002 Metropolitan University seed grant Duke Neurotransgenic Laboratory, supported, in part, with funding from NIH-NINDS Center Core 5P30NS061789
Corresponding Author	Dr. Ricardo Antonio Rossello, Depart­ment of Science and Technology, Sistema Universitario Ana G. Méndez, Metropolitan University, Ave. Ana G. Méndez Cupey, San Juan 00928, Puerto Rico. ricardo.rossello@upr.edu
Key Words	ESRRB; ERAS; Induced stem cells; Over­expression; C-myc
Core Tip	Derivation and culturing of mouse embryonic stem cells (ESCs) from gene targeting to injection into blastocysts for chimera generation is a lengthy process that is difficult to control. Some stem cells might be in a primed state, having lost some of their characteristics, most importantly their pluripotency. These differences between ESC clones are usually only detected after many months by the failure of chimeric males to transmit the ESC genome through the germline. Here we have determined key expression differences between cells in a primed state and those in a presumed ground state. Detection of these differences will give researchers a powerful tool to quickly distinguish these cells, saving time, money and effort by choosing the best clones to go forward with. Furthermore, we were able to rescue the ground state through overexpression, indicating that the fate of these cells may potentially be controlled.
Publish Date	2016-10-20 21:54
Citation	Rossello RA, Pfenning A, Howard JT, Hochgeschwender U. Characterization and genetic manipulation of primed stem cells into a functional naïve state with ESRRB. World J Stem Cells 2016; 8(10): 355-366
URL	http://www.wjgnet.com/1948-0210/full/v8/i10/355.htm
DOI	http://dx.doi.org/10.4252/wjsc.v8.i10.355

Full Article (PDF)	WJSC-8-355.pdf
Full Article (Word)	WJSC-8-355.doc
Manuscript File	24267-Review.docx
Answering Reviewers	24267-Answering reviewers.pdf
Audio Core Tip	24267-Audio core tip.m4a
Biostatistics Review Certificate	24267-Biostatistics statement.pdf
Clinical Trial Registration Statement	DeclarationOf_HelsinkiConformation_Statement_20160114221447.pdf
Conflict-of-Interest Disclosure Form	24267-Conflict-of-interest statement.pdf
Copyright License Agreement	24267-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	24267-Grant application form(s).pdf
Signed Informed Consent Form(s) or Document(s)	Informed_Consent_Statement_20160114220648.pdf
Institutional Animal Care and Use Committee Approval Form or Document	24267-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	24267-Institutional review board statement.pdf
Peer-review Report	24267-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	24267-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	24267-Scientific misconduct check.pdf
Scientific Editor Work List	24267-Scientific editor work list.pdf