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1/20/2017 3:19:00 PM | Browse: 419 | Download: 863
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Received |
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2016-10-11 09:00 |
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Peer-Review Started |
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2016-10-11 21:25 |
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To Make the First Decision |
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2016-12-01 15:40 |
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Return for Revision |
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2016-12-02 17:14 |
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Revised |
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2016-12-05 20:06 |
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Second Decision |
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2016-12-29 10:49 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Company Editor-in-Chief |
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2017-01-04 16:29 |
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Articles in Press |
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2017-01-04 16:29 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2017-01-10 13:32 |
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Publish the Manuscript Online |
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2017-01-19 11:16 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Basic Study |
| Article Title |
Mutational analysis of hepatitis E virus ORF1 "Y-domain": Effects on RNA replication and virion infectivity
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Mohammad Khalid Parvez |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Deanship of Scientific Research at King Saud University, Riyadh |
RG-1435-053 |
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| Corresponding Author |
Mohammad Khalid Parvez, PhD, Associate Professor, Department of Pharmacognosy, King Saud University College of Pharmacy, PO Box 2457, Riyadh 11451,
Saudi Arabia. khalid_parvez@yahoo.com |
| Key Words |
Hepatitis E virus; Open reading frame 1; Y-domain; Palmitoylation; α-helix |
| Core Tip |
The function of hepatitis E virus (HEV) Y-domain remains elusive. In silico analysis of closely-related virus sequences mapped a potential palmitoylation-site (CC) and α-helix segment (LYSWLFE) in the Y-domain. Mutant replicons of the universally conserved residues C336, C337 and W413 showed non-viability. Saturation mutations in the Y-domain (nucleotide sequences 788-994) severely affected RNA replication, revealing their post-transcriptional indispensability. Notably, the three residues cor-responded to the non-conserved codons, indicating their post-translational essentiality. RNA secondary structure prediction showed hairpin formations by the critical bases (788-994), where mutations drastically affected virion infectivity. This is the first demonstration of the critical role of Y-domain sequences in HEV life cycle that warrants further molecular/biochemical studies.
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| Publish Date |
2017-01-19 11:16 |
| Citation |
Parvez MK. Mutational analysis of hepatitis E virus ORF1 “Y-domain”: Effects on RNA replication and virion infectivity. World J Gastroenterol 2017; 23(4): 590-602 |
| URL |
http://www.wjgnet.com/1007-9327/full/v23/i4/590.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v23.i4.590 |
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