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Publication Name World Journal of Stem Cells
Manuscript ID 47987
Country/Territory Japan
2019-04-01 01:55
Peer-Review Started
2019-04-03 09:49
To Make the First Decision
2019-08-28 05:22
Return for Revision
2019-09-02 09:04
2019-09-30 10:30
Second Decision
2019-12-13 11:31
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2019-12-15 06:25
Articles in Press
2019-12-15 06:25
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2019-12-25 01:29
Publish the Manuscript Online
2019-12-30 01:55
ISSN 1948-0210 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Cell Biology
Manuscript Type Review
Article Title Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach
Manuscript Source Invited Manuscript
All Author List Ning-Ning Guo, Li-Ping Liu, Yun-Wen Zheng and Yu-Mei Li
Funding Agency and Grant Number
Funding Agency Grant Number
National Natural Science Foundation of China 81573053
National Natural Science Foundation of China 81770621
Ministry of Education, Culture, Sports, Science, and Technology of Japan, KAKENHI 16K15604
Ministry of Education, Culture, Sports, Science, and Technology of Japan, KAKENHI 18H02866
Natural Science Foundation of Jiangsu Province BK20180281
Corresponding author Yun-Wen Zheng, PhD, Associate Professor, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, University of Tsukuba Faculty of Medicine, Tennodai 1-1-1, Tsukuba 305-8575, Ibaraki, Japan. ywzheng@md.tsukuba.ac.jp
Keywords Induced pluripotent stem cells; Suspension culture; Embryoid body; Early prediction; Committed differentiation; Heterogeneity
Core Tip Several factors lead to uncertain and unpredictable effects in iPSC culture and differentiation, which increase the risk of differentiation failure and cause the wastage of resources. Meanwhile, embryoid body (EB)-mediated differentiation is critical for iPSC-derived cell generation. Here, we summarize and update the approaches of EB formation in recent years, together with our experiences in the laboratory, to demonstrate the key characteristics and practical differentiation of hiPSCs in applications.
Publish Date 2019-12-30 01:55
Citation Guo NN, Liu LP, Zheng YW, Li YM. Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach. World J Stem Cells 2020; 12(1): 25-34
Url https://www.wjgnet.com/1948-0210/full/v12/i1/25.htm
DOI https://dx.doi.org/10.4252/wjsc.v12.i1.25
Full Article (PDF) WJSC-12-25.pdf
Full Article (Word) WJSC-12-25.docx
Manuscript File 47987-Review.docx
Answering Reviewers 47987-Answering reviewers.pdf
Audio Core Tip 47987-Audio core tip.m4a
Conflict-of-Interest Disclosure Form 47987-Conflict-of-interest statement.pdf
Copyright License Agreement 47987-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 47987-Grant application form(s).pdf
Non-Native Speakers of English Editing Certificate 47987-Language certificate.pdf
Peer-review Report 47987-Peer-review(s).pdf
Scientific Misconduct Check 47987-Scientific misconduct check.pdf
Scientific Editor Work List 47987-Scientific editor work list.pdf