| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Engineering, Biomedical |
| Manuscript Type |
Basic Study |
| Article Title |
Creating rat hepatocyte organoid as an in vitro model for drug testing
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Yu-Ting He, Xing-Long Zhu, Sheng-Fu Li, Bing-Qi Zhang, Yi Li, Qiong Wu, Yun-Lin Zhang, Yan-Yan Zhou, Li Li, Ya-Na Qi, Ji Bao and Hong Bu |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
81770618 |
| Key R&D (Major Science and Technology Project) Project of Sichuan Science and Technology Department |
2019YFS0138 |
| Technological Innovation Project of Chengdu New Industrial Technology Research Institute |
2018-CY02-00046-GX |
| the 1.3.5 Project for Disciplines of Excellence, West China Hospital |
ZYGD18012 |
|
| Corresponding Author |
Ji Bao, MD, PhD, Associate Professor, Professor, Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHC, West China Hospital, Sichuan University, No. 37, Guoxue Alley, Wuhou District, Chengdu 610041, Sichuan Province, China. baoji@scu.edu.cn |
| Key Words |
Organoid; Primary hepatocytes; Stem cells; Liver therapies; Extracellular matrix; Drug screening |
| Core Tip |
Mesenchymal stem cells (MSCs) can help primary hepatocytes to create hepatocyte organoids by interacting with a liver-derived extracellular matrix. MSCs and hepatocytes self-assembled together into hepatocyte organoids via MSC-derived condensation related to myosin-II regulatory light chain. The hepatocyte organoids can survive for a long time and maintain the functionality of the hepatocytes while avoiding the limitation of rapid function loss of primary hepatocytes in vitro. This hepatocyte organoid technology can also be used to develop models for liver disease and drug screening. |
| Publish Date |
2020-10-26 12:01 |
| Citation |
He YT, Zhu XL, Li SF, Zhang BQ, Li Y, Wu Q, Zhang YL, Zhou YY, Li L, Qi YN, Bao J, Bu H. Creating rat hepatocyte organoid as an in vitro model for drug testing. World J Stem Cells 2020; In press |
| URL |
https://www.wjgnet.com/1948-0210/full/v12/i10/1184.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v12.i10.1184 |
