Publication Name	World Journal of Biological Chemistry
Manuscript ID	66383
Country	France

Received	2021-03-26 15:36
Peer-Review Started	2021-03-26 15:40
To Make the First Decision
Return for Revision	2021-07-08 07:17
Revised	2021-07-09 10:38
Second Decision	2022-01-13 05:35
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2022-01-14 06:34
Articles in Press	2022-01-14 06:34
Publication Fee Transferred
Edit the Manuscript by Language Editor	2021-12-01 02:24
Typeset the Manuscript	2022-01-19 17:20
Publish the Manuscript Online	2022-01-22 06:08

ISSN	1949-8454 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Copyright	© The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Endocrinology & Metabolism
Manuscript Type	Basic Study
Article Title	Increased monoamine oxidase activity and imidazoline binding sites in insulin-resistant adipocytes from obese Zucker rats
Manuscript Source	Invited Manuscript
All Author List	Christian Carpéné, Luc Marti and Nathalie Morin
Funding Agency and Grant Number	Funding Agency Grant Number Recurrent Grants from Institut National de la Santé et de la Recherche Médicale to the INSERM U1048
Corresponding Author	Christian Carpéné, PhD, Senior Researcher, Institut des Maladies Métaboliques et Cardiovasculaires, INSERM, CHU Rangueil, Bat. L4, BP 84225 , Toulouse 31342, France. christian.carpene@inserm.fr
Key Words	Obesity; Adipocyte; Amine oxidases; Imidazoline binding sites; Creatine kinase B; Idazoxan; Lipogenesis; Hydrogen peroxide; Glucose uptake
Core Tip	The substrates of monoamine oxidase (MAO) and semicarbazide-sensitive amine oxidase (SSAO) partly reproduce the stimulatory effect of insulin on sugar entry in rat fat cells. Especially when combined with vanadium, tyramine and benzylamine mimic more than 70% of the insulin stimulation of glucose uptake. Unfortunately, such insulin activation of glucose utilization is strongly diminished in the genetically obese Zucker rat, an animal model of the metabolic syndrome. In this insulin-resistant and obese rat, the stimulation of glucose transport by tyramine is decreased as much as that of insulin, while the effect of benzylamine is even more altered. SSAO, responsible for benzylamine oxidation, is down-regulated in adipocytes from obese Zucker rats. In contrast, MAO, which predominantly supports the oxidation of tyramine, is increased, apparently not in a sufficient manner to reach the same hexose uptake activation seen in fat cells from lean rats. However, this greater ability to oxidize tyramine was found only in white adipocytes from obese rats since no change was found in the liver or skeletal muscle when compared to lean controls. Moreover, increased MAO activity in the large adipocytes of obese rats was accompanied by an enlarged number of imidazoline binding sites, previously described to be located on MAO. Since the repeated subcutaneous administration of tyramine plus vanadate is somewhat capable of reducing plasma glucose and triglycerides in obese rats, SSAO, MAO, and its associated imidazoline I2 sites deserve further studies with respect to their mimicking insulin action on glucose and lipid metabolism in fat cells.
Publish Date	2022-01-22 06:08
Citation	Carpéné C, Marti L, Morin N. Increased monoamine oxidase activity and imidazoline binding sites in insulin-resistant adipocytes from obese Zucker rats. World J Biol Chem 2022; 13(1): 15-34
URL	https://www.wjgnet.com/1949-8454/full/v13/i1/15.htm
DOI	https://dx.doi.org/10.4331/wjbc.v13.i1.15

Full Article (PDF)	WJBC-13-15.pdf
Full Article (Word)	WJBC-13-15.docx
The ARRIVE Guidelines	66383-The-ARRIVE-Guidelines-revision.pdf
Manuscript File	66383_Auto_Edited.docx
Answering Reviewers	66383-Answering reviewers.pdf
Audio Core Tip	66383-Audio core tip.mp4
Biostatistics Review Certificate	66383-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	66383-Conflict-of-interest statement.pdf
Copyright License Agreement	66383-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	66383-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	66383-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	66383-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	66383-Language certificate.pdf
Peer-review Report	66383-Peer-review(s).pdf
Scientific Misconduct Check	66383-Bing-Liu M-1.png
Scientific Editor Work List	66383-Scientific editor work list.pdf
CrossCheck Report	66383-CrossCheck report.pdf