Publication Name	World Journal of Gastroenterology
Manuscript ID	75445
Country	Germany

Received	2022-01-28 11:16
Peer-Review Started	2022-01-28 11:18
First Decision by Editorial Office Director	2022-04-10 20:32
Return for Revision	2022-04-10 20:32
Revised	2022-05-20 08:36
Publication Fee Transferred
Second Decision by Editor	2022-07-11 02:52
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2022-07-11 16:06
Articles in Press	2022-07-11 16:06
Edit the Manuscript by Language Editor	2022-07-03 21:38
Typeset the Manuscript	2022-07-25 02:52
Publish the Manuscript Online	2022-08-03 07:54

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
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Category	Oncology
Manuscript Type	Case Control Study
Article Title	Comprehensive evaluation of microRNA as a biomarker for the diagnosis of hepatocellular carcinoma
Manuscript Source	Unsolicited Manuscript
All Author List	Juliane Malik, Martin Klammer, Vinzent Rolny, Henry Lik-Yuen Chan, Teerha Piratvisuth, Tawesak Tanwandee, Satawat Thongsawat, Wattana Sukeepaisarnjaroen, Juan Ignacio Esteban, Marta Bes, Bruno Köhler and Magdalena Swiatek-de Lange
Funding Agency and Grant Number
Corresponding Author	Juliane Malik, PhD, N/A, N/A, N/A, Roche Diagnostics GmbH, Nonnenwald 2, Penzberg 82377, Germany. juliane.malik@roche.com
Key Words	Carcinoma; Hepatocellular; MicroRNAs; Biomarkers; Alpha-fetoprotein; Protein induced by vitamin K absence-II; Diagnosis
Core Tip	Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and has a high mortality rate, making early diagnosis essential for better treatment outcomes. Studies have shown microRNAs to be early markers for HCC; we evaluated the potential diagnostic role of microRNAs alone and in combination with known protein biomarkers (alpha-fetoprotein, protein induced by vitamin K absence/antagonist-II) in samples from HCC-affected individuals and controls using real-time quantitative PCR and next-generation sequencing. MiR-21 and miR-423 demonstrated significant differential expression between the HCC and control groups. MicroRNAs alone or as a signature in combination with protein biomarkers did not improve diagnostic performance of the protein biomarkers.
Publish Date	2022-08-03 07:54
Citation	Malik J, Klammer M, Rolny V, Chan HLY, Piratvisuth T, Tanwandee T, Thongsawat S, Sukeepaisarnjaroen W, Esteban JI, Bes M, Köhler B, Swiatek-de Lange M. Comprehensive evaluation of microRNA as a biomarker for the diagnosis of hepatocellular carcinoma. World J Gastroenterol 2022; 28(29): 3917-3933
URL	https://www.wjgnet.com/1007-9327/full/v28/i29/3917.htm
DOI	https://dx.doi.org/10.3748/wjg.v28.i29.3917

Full Article (PDF)	WJG-28-3917.pdf
Full Article (Word)	WJG-28-3917.docx
STROBE Statement	75445-STROBE-Statement-revision.doc
Manuscript File	75445_Auto_Edited-LM.docx
Answering Reviewers	75445-Answering reviewers.pdf
Audio Core Tip	75445-Audio core tip.wav
Biostatistics Review Certificate	75445-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	75445-Conflict-of-interest statement.pdf
Copyright License Agreement	75445-Copyright license agreement.pdf
Signed Informed Consent Form(s) or Document(s)	75445-Informed consent statement.pdf
Institutional Review Board Approval Form or Document	75445-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	75445-Language certificate.pdf
Supplementary Material	75445-Supplementary material.pdf
Peer-review Report	75445-Peer-review(s).pdf
Scientific Misconduct Check	75445-Bing-Chang KL-2.png
Scientific Editor Work List	75445-Scientific editor work list.pdf
CrossCheck Report	75445-CrossCheck report.pdf