Publication Name	World Journal of Methodology
Manuscript ID	7840
Country	Germany

Received	2013-12-02 19:21
Peer-Review Started	2013-12-04 14:53
First Decision by Editorial Office Director
Return for Revision	2014-02-20 19:59
Revised	2014-09-11 00:00
Publication Fee Transferred
Second Decision by Editor	2014-10-16 15:24
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2014-10-16 16:11
Articles in Press	2014-10-16 16:11
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-12-11 15:54
Publish the Manuscript Online	2014-12-23 15:34

ISSN	2222-0682 (online)
Open Access
Copyright
Article Reprints	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Medical Laboratory Technology
Manuscript Type	Autobiography
Article Title	Methodical and pre-analytical characteristics of a multiplex cancer biomarker immunoassay
Manuscript Source	Invited Manuscript
All Author List	Natalie Hermann, Katja Dreßen, Frank A Schildberg, Christopher Jakobs and Stefan Holdenrieder
Funding Agency and Grant Number
Corresponding Author	Stefan Holdenrieder, MD, Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Sigmund-Freud Str. 15, 53127 Bonn, Germany. stefan.holdenrieder@uni-bonn.de
Key Words	Multiplex immunoassay; Tumor marker; Cytokines; Cell death markers; Methodical evaluation
Core Tip	In this study, the methodological quality of a new research-use-only multiplex magnetic bead assay, particularly designed for cancer diagnosis, was evaluated. This attractive panel includes 24 biomarkers: established as well as auspicious tumor markers and markers deriving from the fields of apoptosis, immunology and angiogenesis. Herewith, the complexity and multifactorial background of a cancer disease is depicted. Measurements were performed with physiological serum pools and intra- and inter-assay imprecision as well as dilution linearity were assessed. Furthermore, the influence of preanalytical factors was investigated.
Publish Date	2014-12-23 15:34
Citation	Hermann N, Dreßen K, Schildberg FA, Jakobs C, Holdenrieder S. Methodical and pre-analytical characteristics of a multiplex cancer biomarker immunoassay. World J Methodol 2014; 4(4): 219-231
URL	http://www.wjgnet.com/2222-0682/full/v4/i4/219.htm
DOI	http://dx.doi.org/10.5662/wjm.v4.i4.219

Full Article (PDF)	WJM-4-219.pdf
Full Article (Word)	WJM-4-219.doc
Manuscript File	7840-Review.docx
Answering Reviewers	7840-Answering reviewers.pdf
Copyright License Agreement	7840-Copyright assignment.pdf
Peer-review Report	7840-Peer review(s).pdf
Scientific Misconduct Check	7840-CrossCheck.jpg
Scientific Editor Work List	7840-Scientific editor work list.pdf