Publication Name	World Journal of Gastroenterology
Manuscript ID	80836
Country	South Korea

Received	2022-10-14 06:34
Peer-Review Started	2022-10-14 06:35
To Make the First Decision
Return for Revision	2023-01-03 20:42
Revised	2023-01-13 08:35
Second Decision	2023-02-20 03:45
Accepted by Journal Editor-in-Chief	2023-02-21 02:53
Accepted by Company Editor-in-Chief	2023-02-27 22:14
Articles in Press	2023-02-27 22:14
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2023-03-06 02:59
Publish the Manuscript Online	2023-03-17 06:20

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I
Manuscript Source	Unsolicited Manuscript
All Author List	Kijeong Kim, Yu-Min Choi, Dong Hyun Kim, Junghwa Jang, Won Hyeok Choe and Bum-Joon Kim
Funding Agency and Grant Number	Funding Agency Grant Number National Research Foundation of Korea 2022R1A2B5B01001421 Korea Health Technology R&D Project through the Korea Health Industry Development Institute, the Ministry of Health & Welfare, Republic of Korea HI22C0476
Corresponding Author	Bum-Joon Kim, PhD, Professor, Department of Microbiology and Immunology, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, South Korea. kbumjoon@snu.ac.kr
Key Words	Hepatitis B virus; Genotype C2; Polymerase; rt269; Locked nucleic acid-real time-polymerase chain reaction; Chronic hepatitis B
Core Tip	Hepatitis B virus (HBV) genotype C2 infections have distinct clinical or virological traits, including a higher risk of hepatocellular carcinoma, lower response rate to interferon or prolonged hepatitis B e antigen-positive phase. We recently reported that the presence of two HBV Pol RT polymorphisms, rt269L and rt269I, contributed to unique traits of HBV genotype C2. Here, instead of time- or labor-consuming direct sequencing, we developed a new locked nucleic acid (LNA)-real time-polymerase chain reaction (RT-PCR) method for the separation between rt269L (L1 and L2) and I type from Korean chronic hepatitis B patients of genotype C2. The newly developed LNA-RT-PCR could be effectively used for the understanding of epidemiology and disease progression in genotype C2 endemic areas.
Publish Date	2023-03-17 06:20
Citation	Kim K, Choi YM, Kim DH, Jang J, Choe WH, Kim BJ. Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I. World J Gastroenterol 2023; 29(11): 1721-1734
URL	https://www.wjgnet.com/1007-9327/full/v29/i11/1721.htm
DOI	https://dx.doi.org/10.3748/wjg.v29.i11.1721

Full Article (PDF)	WJG-29-1721.pdf
Full Article (Word)	WJG-29-1721.docx
Manuscript File	80836_Auto_Edited-LM.docx
Answering Reviewers	80836-Answering reviewers.pdf
Audio Core Tip	80836-Audio core tip.mp3
Biostatistics Review Certificate	80836-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	80836-Conflict-of-interest statement.pdf
Copyright License Agreement	80836-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	80836-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	80836-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	80836-Language certificate.pdf
Supplementary Material	80836-Supplementary material.pdf
Peer-review Report	80836-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	80836-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	80836-Bing-Wang JJ-2.png
Scientific Editor Work List	80836-Scientific editor work list.pdf