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3/17/2023 7:26:34 AM | Browse: 27 | Download: 46
Publication Name World Journal of Gastroenterology
Manuscript ID 80836
Country/Territory South Korea
2022-10-14 06:34
Peer-Review Started
2022-10-14 06:35
To Make the First Decision
Return for Revision
2023-01-03 20:42
2023-01-13 08:35
Second Decision
2023-02-20 03:45
Accepted by Journal Editor-in-Chief
2023-02-21 02:53
Accepted by Company Editor-in-Chief
2023-02-27 22:14
Articles in Press
2023-02-27 22:14
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2023-03-06 02:59
Publish the Manuscript Online
2023-03-17 06:20
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright © The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Gastroenterology and Hepatology
Manuscript Type Basic Study
Article Title Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I
Manuscript Source Unsolicited Manuscript
All Author List Kijeong Kim, Yu-Min Choi, Dong Hyun Kim, Junghwa Jang, Won Hyeok Choe and Bum-Joon Kim
Funding Agency and Grant Number
Funding Agency Grant Number
National Research Foundation of Korea 2022R1A2B5B01001421
Korea Health Technology R&D Project through the Korea Health Industry Development Institute, the Ministry of Health & Welfare, Republic of Korea HI22C0476
Corresponding Author Bum-Joon Kim, PhD, Professor, Department of Microbiology and Immunology, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, South Korea. kbumjoon@snu.ac.kr
Key Words Hepatitis B virus; Genotype C2; Polymerase; rt269; Locked nucleic acid-real time-polymerase chain reaction; Chronic hepatitis B
Core Tip Hepatitis B virus (HBV) genotype C2 infections have distinct clinical or virological traits, including a higher risk of hepatocellular carcinoma, lower response rate to interferon or prolonged hepatitis B e antigen-positive phase. We recently reported that the presence of two HBV Pol RT polymorphisms, rt269L and rt269I, contributed to unique traits of HBV genotype C2. Here, instead of time- or labor-consuming direct sequencing, we developed a new locked nucleic acid (LNA)-real time-polymerase chain reaction (RT-PCR) method for the separation between rt269L (L1 and L2) and I type from Korean chronic hepatitis B patients of genotype C2. The newly developed LNA-RT-PCR could be effectively used for the understanding of epidemiology and disease progression in genotype C2 endemic areas.
Publish Date 2023-03-17 06:20
Citation Kim K, Choi YM, Kim DH, Jang J, Choe WH, Kim BJ. Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I. World J Gastroenterol 2023; 29(11): 1721-1734
URL https://www.wjgnet.com/1007-9327/full/v29/i11/1721.htm
DOI https://dx.doi.org/10.3748/wjg.v29.i11.1721
Full Article (PDF) WJG-29-1721.pdf
Full Article (Word) WJG-29-1721.docx
Manuscript File 80836_Auto_Edited-LM.docx
Answering Reviewers 80836-Answering reviewers.pdf
Audio Core Tip 80836-Audio core tip.mp3
Biostatistics Review Certificate 80836-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 80836-Conflict-of-interest statement.pdf
Copyright License Agreement 80836-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 80836-Grant application form(s).pdf
Institutional Review Board Approval Form or Document 80836-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 80836-Language certificate.pdf
Supplementary Material 80836-Supplementary material.pdf
Peer-review Report 80836-Peer-review(s).pdf
Journal Editor-in-Chief Review Report 80836-Journal editor-in-chief review report.pdf
Scientific Misconduct Check 80836-Bing-Wang JJ-2.png
Scientific Editor Work List 80836-Scientific editor work list.pdf