Publication Name	World Journal of Hepatology
Manuscript ID	81123
Country	Malaysia

Received	2022-10-27 06:03
Peer-Review Started	2022-10-27 06:07
To Make the First Decision
Return for Revision	2022-12-31 06:40
Revised	2023-01-17 07:01
Second Decision	2023-03-03 03:22
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2023-03-03 09:01
Articles in Press	2023-03-03 09:01
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2023-03-07 00:51
Publish the Manuscript Online	2023-03-22 11:20

ISSN	1948-5182 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Copyright	© The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Nanoscience & Nanotechnology
Manuscript Type	Basic Study
Article Title	mRNA transcriptome profiling of human hepatocellular carcinoma cells HepG2 treated with Catharanthus roseus-silver nanoparticles
Manuscript Source	Invited Manuscript
All Author List	Nur Asna Azhar, Siti Aishah Abu Bakar, Marimuthu Citartan and Nor Hazwani Ahmad
Funding Agency and Grant Number	Funding Agency Grant Number Ministry of Higher Education Malaysia FRGS/1/2015/SG03/USM/03/1
Corresponding Author	Nor Hazwani Ahmad, PhD, Senior Lecturer, Department of Biomedical Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Jalan Tun Hamdan Sheikh Tahir, Kepala Batas 13200, Pulau Pinang, Malaysia. norhazwani@usm.my
Key Words	Catharanthus roseus; HepG2; Silver nanoparticles; Transcriptome; oxidative stress; Apoptosis; Cell cycle
Core Tip	Despite the increased attention on cancer nanomedicine which is advantageous to overcome the limitations of conventional cancer treatment, the information on the selectivity and detailed mechanisms at the cellular and molecular level remain unclear. To evaluate its selectivity effects, the proliferative activity of both liver cancer cells HepG2 and normal liver cells THLE-3 in response to Catharanthus roseus-silver nanoparticles (C. roseus-AgNPs) was assessed. To determine the possible signalling pathways induced by the C. roseus-AgNPs, the mRNA transcriptome profiling of hepatocellular carcinoma cell line HepG2 was performed, highlighting the expression of genes associated with oxidative stress, apoptosis, and cell cycle arrest. The elucidation of its selectivity effects and detailed wide genome screening would enlighten the cellular and molecular signalling pathways and provide a strong basis towards the development of C. roseus-AgNPs as an anticancer drug for liver cancer.
Publish Date	2023-03-22 11:20
Citation	Azhar NA, Abu Bakar SA, Citartan M, Ahmad NH. mRNA transcriptome profiling of human hepatocellular carcinoma cells HepG2 treated with Catharanthus roseus-silver nanoparticles. World J Hepatol 2023; 15(3): 393-409
URL	https://www.wjgnet.com/1948-5182/full/v15/i3/393.htm
DOI	https://dx.doi.org/10.4254/wjh.v15.i3.393

Full Article (PDF)	WJH-15-393-with-cover.pdf
Full Article (Word)	WJH-15-393.docx
Manuscript File	81123_Auto_Edited-JLW.docx
Answering Reviewers	81123-Answering reviewers.pdf
Audio Core Tip	81123-Audio core tip.mp3
Biostatistics Review Certificate	81123-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	81123-Conflict-of-interest statement.pdf
Copyright License Agreement	81123-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	81123-Grant application form(s).pdf
Non-Native Speakers of English Editing Certificate	81123-Language certificate.pdf
Supplementary Material	81123-Supplementary material.pdf
Peer-review Report	81123-Peer-review(s).pdf
Scientific Misconduct Check	81123-Bing-Chang KL-2.png
Scientific Editor Work List	81123-Scientific editor work list.pdf