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6/27/2023 6:08:55 AM | Browse: 116 | Download: 359
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Received |
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2023-03-21 10:16 |
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Peer-Review Started |
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2023-03-21 10:16 |
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To Make the First Decision |
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Return for Revision |
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2023-04-09 23:26 |
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Revised |
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2023-04-20 03:19 |
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Second Decision |
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2023-04-27 02:50 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Company Editor-in-Chief |
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2023-04-27 06:48 |
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Articles in Press |
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2023-04-27 06:48 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2023-06-16 02:26 |
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Publish the Manuscript Online |
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2023-06-26 14:09 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Culture and identification of neonatal rat brain-derived neural stem cells
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Qing-Zhong Zhou, Xiao-Lan Feng, Xu-Feng Jia, Nurul Huda Binti Mohd Nor, Mohd Hezery Bin Harun, Da-Xiong Feng and Wan Aliaa Wan Sulaiman |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Project of Sichuan Department of Science and Technology |
2016PJ552 |
| Project of Luzhou Department of Science and Technology |
2016-R-70(18/24) |
| Project of Southwest Medical University of Science and Technology |
15073 |
| Project of Southwest Medical University of Science and Technology |
2015-YJ021 |
| Orthopaedic diseases (Shang Antong) special research Project of Sichuan Medical Association |
20220206070192 |
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| Corresponding Author |
Wan Aliaa Wan Sulaiman, BMed, MBChB, MRCP, Senior Lecturer, Department of Neurology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Level 3, Administration Building, Serdang 43400, Selangor, Malaysia. wanaliaa@upm.edu.my |
| Key Words |
Neonatal rats; Brain-derived neural stem cells; Culture; Identification |
| Core Tip |
How to harvest sufficient neural stem cells (NSCs) is a basic requirement for the study and clinical application of NSCs. This study describes a simplified and efficient method for neonatal rat brain-derived NSC culture and identification comprehensively considering the influencing factors including timing of passaging, passage number, passaging approaches and methods for cell identification. It demonstrates that combination of TrypLTM Express and mechanical tapping and pipetting techniques makes a more efficient way of passaging. The optimal timing for NSC passage is on the fourth to fifth day of primary or passage NSC culture. |
| Publish Date |
2023-06-26 14:09 |
| Citation |
Zhou QZ, Feng XL, Jia XF, Mohd Nor NHB, Harun MHB, Feng DX, Wan Sulaiman WA. Culture and identification of neonatal rat brain-derived neural stem cells. World J Stem Cells 2023; 15(6): 607-616 |
| URL |
https://www.wjgnet.com/1948-0210/full/v15/i6/607.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v15.i6.607 |
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