Publication Name	World Journal of Medical Genetics
Manuscript ID	8499
Country	Japan

Received	2013-12-29 15:16
Peer-Review Started	2013-12-29 20:15
To Make the First Decision	2014-02-27 10:53
Return for Revision	2014-03-04 20:56
Revised	2014-03-12 10:24
Second Decision	2014-05-19 09:05
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-05-19 09:19
Articles in Press	2014-05-23 12:25
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-09-03 17:47
Publish the Manuscript Online	2014-09-19 20:46

ISSN	2220-3184 (online)
Open Access
Copyright
Article Reprints	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Genetics & Heredity
Manuscript Type	Review
Article Title	Genome engineering using the CRISPR/Cas system
Manuscript Source	Invited Manuscript
All Author List	Takuro Horii and Izuho Hatada
Funding Agency and Grant Number	Funding Agency Grant Number Ministry of Education, Culture, Sports, Science and Technology of Japan Ministry of Health, Labour and Welfare of Japan ational Institute of Biomedical Innovation Asahi Glass Foundation Ichiro Kanehara Foundation Program for Cultivating Global Leaders in Heavy Ion Therapeutics and Engineering
Corresponding Author	Izuho Hatada, PhD, Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma 371-8512, Japan. hatada@gunma-u.ac.jp
Key Words	Clustered regularly at interspaced short palindromic repeats; Clustered regularly at interspaced short palindromic repeats associated 9; Genome engineering; Double-strand breaks; Non-homologous end joining; Homology-directed repair
Core Tip	This review introduces the latest information about the genome manipulation technology of the clustered regularly at interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system to readers. We focus particularly on the application of CRISPR/Cas in mammalian cultured cells and mice. The problems of off-target effects and the prospects for therapeutic applications of CRISPR/Cas in the future are also discussed.
Publish Date	2014-09-19 20:46
Citation	Horii T, Hatada I. Genome engineering using the CRISPR/Cas system. World J Med Genet 2014; 4(3): 69-76
URL	http://www.wjgnet.com/2220-3184/full/v4/i3/69.htm
DOI	http://dx.doi.org/10.5496/wjmg.v4.i3.69

Full Article (PDF)	WJMG-4-69.pdf
Full Article (Word)	WJMG-4-69.doc
Manuscript File	8499-Review.docx
Answering Reviewers	8499-Answering reviewers.pdf
Copyright License Agreement	8499-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	8499-Language certificate.pdf
Peer-review Report	8499-Peer review(s).pdf
Scientific Editor Work List	8499-Scientific editor work list.pdf