Publication Name	World Journal of Methodology
Manuscript ID	87001
Country	China

Received	2023-07-18 07:43
Peer-Review Started	2023-07-18 07:46
To Make the First Decision
Return for Revision	2023-09-13 07:33
Revised	2023-09-25 05:47
Second Decision	2023-10-13 14:27
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2023-10-16 08:16
Articles in Press	2023-10-16 08:16
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2023-12-13 07:51
Publish the Manuscript Online	2023-12-20 14:52

ISSN	2222-0682 (online)
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Category	Biochemical Research Methods
Manuscript Type	Basic Study
Article Title	Sequential extraction of RNA, DNA and protein from cultured cells of the same group
Manuscript Source	Invited Manuscript
All Author List	Ying-Yu Cui
Funding Agency and Grant Number	Funding Agency Grant Number Postdoctoral Science Foundation of China 2005038300 National Natural Science Foundation of China 30671028
Corresponding Author	Ying-Yu Cui, PhD, Associate Professor, Teacher, Department of Cell Biology, Institute of Medical Genetics, Tongji University School of Medicine, No. 500, Zhennan Road Putuo District, Shanghai 200331, China. yycui@tongji.edu.cn
Key Words	Sequential extraction; Ribonucleic acid; Deoxyribonucleic acid; Protein; Cultured cells
Core Tip	Sequential extraction of nucleic acids and proteins from cultured cells of the same group. Life is a way of material (mainly protein and nucleic acid) movement, and health lies in movement. Cell is the most fundamental structural and functional unit of life. Therefore, the effective isolation of nucleic acids and proteins from cells is the foundation and prerequisite for revealing the mysteries of life. However, during laboratory routine for isolation of nucleic acids and proteins, cell samples are often from different culture dishes, usually leading to inevitable experimental errors and sometimes poor repeatability. The present research tries to explore the possibility to simultaneously isolate nucleic acids and proteins from the same sample, while reducing experimental errors and ensuring consistency during experimentation. The present study established a selective protocol for sequential isolation of RNA, DNA and proteins from the same cells with the characteristics of easy operation, rapid extraction and high efficiency. RNAzol reagent was used for the sequential isolation of RNA, DNA, and proteins from the same cultured HepG2 cells, resulting in a novel protocol containing four steps. A protocol for sequential isolation of RNA, DNA and proteins was established and the procedure takes as few as 3–4 d from the start to quality verification and is highly efficient. The quality of RNA, DNA and proteins isolated through sequential isolation protocol can be used for reverse transcription - polymerase chain reaction (PCR), PCR and western blot, respectively. The present procedure is not only easy, rapid and high efficient, but also economical and practical, especially for researchers in developing and underdeveloped countries.
Publish Date	2023-12-20 14:52
Citation	Cui YY. Sequential extraction of RNA, DNA and protein from cultured cells of the same group. World J Methodol 2023; 13(5): 484-491
URL	https://www.wjgnet.com/2222-0682/full/v13/i5/484.htm
DOI	https://dx.doi.org/10.5662/wjm.v13.i5.484

Full Article (PDF)	WJM-13-484-with-cover.pdf
Full Article (Word)	WJM-13-484.docx
The ARRIVE Guidelines	87001-The ARRIVE Guidelines-revision.pdf
Manuscript File	87001_Auto_Edited-JLW.docx
Answering Reviewers	87001-Answering reviewers.pdf
Audio Core Tip	87001-Audio core tip.mp3
Biostatistics Review Certificate	87001-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	87001-Conflict-of-interest statement.pdf
Copyright License Agreement	87001-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	87001-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	87001-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	87001-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	87001-Language certificate.pdf
Peer-review Report	87001-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	87001-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	87001-Bing-Li L-2.png
Scientific Editor Work List	87001-Scientific editor work list.pdf
CrossCheck Report	87001-CrossCheck report.pdf
CrossCheck Report	87001-CrossCheck.png