Publication Name	World Journal of Stem Cells
Manuscript ID	90346
Country	Pakistan

Received	2023-11-30 13:00
Peer-Review Started	2023-11-30 13:00
To Make the First Decision
Return for Revision	2024-01-15 08:00
Revised	2024-02-01 04:48
Second Decision	2024-02-29 02:46
Accepted by Journal Editor-in-Chief	2024-03-06 03:44
Accepted by Executive Editor-in-Chief	2024-03-18 06:14
Articles in Press	2024-03-18 06:14
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2024-04-22 00:51
Publish the Manuscript Online	2024-04-25 05:24

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine
Manuscript Source	Invited Manuscript
All Author List	Shafiqa Naeem Rajput, Bushra Kiran Naeem, Anwar Ali, Asmat Salim and Irfan Khan
Funding Agency and Grant Number	Funding Agency Grant Number Higher Education Commission, Islamabad, Pakistan grant 20-17590/NRPU/R&D/HEC/2021 2021
Corresponding Author	Irfan Khan, PhD, Assistant Professor, Center for Regenerative Medicine and Stem Cells Research, The Aga Khan University, Stadium Road, P.O. Box 3500, Karachi 74800, Sindh, Pakistan. irfankhan.bangash@aku.edu
Key Words	Human umbilical cord; Mesenchymal stem cells; Expansion; Cell proliferation; In vitro expansion; Senescence
Core Tip	Therapeutic transplantation of mesenchymal stem cells (MSCs) requires one to four million cells/kg, body weight. Ex vivo expansion of stable MSCs at higher passage numbers limits their clinical applications due to senescence, variation in genetic content and short life span. There is a need to adopt this approach for the preparation of cells for clinical applications. This study results in the development of a cutting-edge protocol for scaling the stem cell population ex vivo in less time. It may rapidly increase the cell number in vitro to fulfill in vivo therapeutic cell doses. This method might decrease immune rejection. Since these MSCs were isolated from the same recultured human umbilical cord, they have persistent MSC stemness and may decrease tissue vs graft rejection due to the less rigorous HLA screening performed in allogenic transplantation, which could make it more cost-effective to uphold good manufacturing practices. Certainly, more studies that are exploratory should be carried out to further elucidate the mechanism via preclinical applications.
Publish Date	2024-04-25 05:24
Citation	Rajput SN, Naeem BK, Ali A, Salim A, Khan I. Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine. World J Stem Cells 2024; 16(4): 410-433
URL	https://www.wjgnet.com/1948-0210/full/v16/i4/410.htm
DOI	https://dx.doi.org/10.4252/wjsc.v16.i4.410

Full Article (PDF)	WJSC-16-410-with-cover.pdf
Manuscript File	90346_Auto_Edited-YJP.docx
Answering Reviewers	90346-Answering reviewers.pdf
Audio Core Tip	90346-Audio core tip.aac
Biostatistics Review Certificate	90346-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	90346-Conflict-of-interest statement.pdf
Copyright License Agreement	90346-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	90346-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	90346-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	90346-Language certificate.pdf
Peer-review Report	90346-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	90346-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	90346-Bing-Wang JJ-2.png
Scientific Editor Work List	90346-Scientific editor work list.pdf
CrossCheck Report	90346-CrossCheck report.pdf