Publication Name	World Journal of Stem Cells
Manuscript ID	90788
Country	China

Received	2023-12-19 07:51
Peer-Review Started	2023-12-19 07:51
To Make the First Decision
Return for Revision	2024-01-12 07:12
Revised	2024-01-21 23:11
Second Decision	2024-02-18 01:42
Accepted by Journal Editor-in-Chief	2024-02-19 22:48
Accepted by Executive Editor-in-Chief	2024-02-28 07:12
Articles in Press	2024-02-28 07:12
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2024-03-18 01:16
Publish the Manuscript Online	2024-03-25 02:43

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright	© The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Dentistry, Oral Surgery & Medicine
Manuscript Type	Basic Study
Article Title	Self-assembly of differentiated dental pulp stem cells facilitates spheroid human dental organoid formation and prevascularization
Manuscript Source	Unsolicited Manuscript
All Author List	Fei Liu, Jing Xiao, Lei-Hui Chen, Yu-Yue Pan, Jun-Zhang Tian, Zhi-Ren Zhang and Xiao-Chun Bai
Funding Agency and Grant Number	Funding Agency Grant Number Science and Technology Programme of Guangzhou City 202201020341
Corresponding Author	Xiao-Chun Bai, PhD, Professor, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, No. 1023-1063 Shatai South Road, Baiyun District, Guangzhou 510515, Guangdong Province, China. baixc15@smu.edu.cn
Key Words	Human dental pulp stem cells; Prevascularized organoids; Integrated analyses; Angiogenesis; Forkhead box protein O1
Core Tip	We demonstrated the three-dimensional self-assembly of adult stem cell-human dermal papilla cells and endothelial cells into a novel type of spheroid-shaped dental pulp organoid in vitro under hypoxia and conditioned medium. These organoids have been constructed to be morphologically and functionally closer to dental pulp tissue. Through the integration and analysis of single-cell RNA sequencing and RNA sequencing data, forkhead box protein O1 and fibroblast growth factor 2 were identified as crucial markers involved in the regulation of organoid angiogenesis. In this innovative study, we effectively established an in vitro model of prevascularized dental pulp organoids and used it to elucidate new mechanisms of angiogenesis during regeneration, facilitating the development of clinical treatment strategies.
Publish Date	2024-03-25 02:43
Citation	Liu F, Xiao J, Chen LH, Pan YY, Tian JZ, Zhang ZR, Bai XC. Self-assembly of differentiated dental pulp stem cells facilitates spheroid human dental organoid formation and prevascularization. World J Stem Cells 2024; 16(3): 287-304
URL	https://www.wjgnet.com/1948-0210/full/v16/i3/287.htm
DOI	https://dx.doi.org/10.4252/wjsc.v16.i3.287

Full Article (PDF)	WJSC-16-287-with-cover.pdf
Manuscript File	90788_Auto_Edited-YJP.docx
Answering Reviewers	90788-Answering reviewers.pdf
Audio Core Tip	90788-Audio core tip.wav
Biostatistics Review Certificate	90788-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	90788-Conflict-of-interest statement.pdf
Copyright License Agreement	90788-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	90788-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	90788-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	90788-Language certificate.pdf
Peer-review Report	90788-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	90788-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	90788-Bing-Wang JJ-2.png
Scientific Editor Work List	90788-Scientific editor work list.pdf
CrossCheck Report	90788-CrossCheck report.pdf