| ISSN |
1948-5182 (online) |
| Open Access |
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. |
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Immunology |
| Manuscript Type |
Basic Study |
| Article Title |
Uncovering immune cell heterogeneity in hepatocellular carcinoma by combining single-cell RNA sequencing with T-cell receptor sequencing
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Xin-Yu Gu, Shuang-Lin Gu, Zi-Yi Chen, Jin-Long Tong, Xiao-Yue Li, Hui Dong, Cai-Yun Zhang, Wen-Xian Qian, Xiu-Chang Ma, Chang-Hua Yi and Yong-Xiang Yi |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| the Scientific Research Topic of Jiangsu Provincial Health Care Commission |
M2021017 |
| the High-level Talent Research Project of the Second Hospital of Nanjing |
0313504 |
| the Nanjing Second Hospital Academic Leader Program |
0313506 |
|
| Corresponding Author |
Yong-Xiang Yi, MD, PhD, Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, No. 321 Zhongshan Road, Gulou Distric, Nanjing 210008, Jiangsu Province, China. ian0126@126.com |
| Key Words |
Single-cell RNA sequencing; Paired T-cell receptor sequencing; Hepatocellular carcinoma; Immune cell subpopulations; Tumor-infiltrating immune cells |
| Core Tip |
In this study, the heterogeneity of immune cell subpopulations, their potential functions, and their possible interactions in hepatocellular carcinoma (HCC) tissues and cancer-adjacent normal tissues were analyzed using single-cell RNA sequencing and T-cell receptor sequencing. Genes that may serve as biomarkers were identified by characterizing the major T-cell populations and 2 newly identified T-cell populations. In combination with the T-cell receptor analysis, we also inferred the clonal expansion characteristics of each T-cell cluster in HCC patients. In conclusion, we identified lymphocyte subpopulations and potential biomarker genes critical for HCC development and revealed the clonal expansion of infiltrating T cells. |
| Publish Date |
2025-02-20 08:39 |
| Citation |
<p>Gu XY, Gu SL, Chen ZY, Tong JL, Li XY, Dong H, Zhang CY, Qian WX, Ma XC, Yi CH, Yi YX. Uncovering immune cell heterogeneity in hepatocellular carcinoma by combining single-cell RNA sequencing with T-cell receptor sequencing. <i>World J Hepatol</i> 2025; 17(2): 99046</p> |
| URL |
https://www.wjgnet.com/1948-5182/full/v17/i2/99046.htm |
| DOI |
https://dx.doi.org/10.4254/wjh.v17.i2.99046 |
