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Name of journal: World Journal of Pharmacology
ESPS Manuscript NO: 15659
Columns: REVIEW
Progress in pancreatic cancer therapeutics: The potential to exploit molecular targets
Thillai K et al. Progress in pancreatic cancer therapeutics
Kiruthikah Thillai, Debashis Sarker, Paul Ross
Kiruthikah Thillai, Debashis Sarker, Paul Ross, Department of Medical Oncology, Guys and St Thomas NHS Trust, SE19RT London, United Kingdom
Author contributions: All authors contributed to this manuscript.
Conflict-of-interest: The authors declare no conflict of interest relevant to this article.
Open-Access: This article is an open-accessarticlewhich was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributedinaccordancewith the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: HYPERLINK "http://creativecommons.org/licenses/by-nc/4.0/" http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Paul Ross, Department of Medical Oncology, Guys and St Thomas NHS Trust, Great Maze Pond, SE19RT London, United Kingdom. paul.ross@gstt.nhs.uk
Telephone: +44-207-1887188
Fax: +44-207-1887534
Received: December 2, 2014
Peer-review started: December 2, 2014
First decision: February 7, 2015
Revised: March 9, 2015
Accepted: April 10, 2015
Article in press:
Published online:
Abstract
Pancreatic ductal adenocarcinoma is an aggressive and devastating disease associated with poor survival outcomes. Even though significant advances have been made towards understanding the intricate pathology of this cancer, several important aspects remain unknown. Recently, key genetic mutations within the tumour have been identified, but the exact role they play in tumourigenesis has yet to be determined. For many years, the micro-tumour environment and stroma was thought to aid proliferation but there is now emerging research that suggests the contrary. Several novel targeted agents in pre-clinical and early clinical studies have been promising but it remains to be seen whether they will have a significant impact on patient outcomes. In this review we discuss the unique nature of pancreatic cancer biology, current treatment options and summarise the latest results from pre-clinical and clinical research. We also discuss the future strategies that are needed to improve outcomes for this disease.
Key words: Pancreatic cancer; Adenocarcinoma; Targeted therapy; Genomics; Stroma; KRAS; Chemotherapy
The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
Core tip: Pancreatic ductal adenocarcinoma is a cancer with several significant genetic aberrations that have recently been identified by international research efforts. Despite these findings, standard therapy for advanced disease consists primarily of chemotherapy. In the last few years two new chemotherapy regimens, FOLFIRINOX and Gemcitabine/Nab-paclitaxel, have demonstrated survival benefits in large phase III trials resulting in a change to current practise. However, the advent of targeted treatments has not yet had a significant impact in this disease compared with other malignancies. Current research strategies include developing therapies directed towards the RAS-RAK-MEK pathway, PI3K-AKT-mTOR pathway, notch pathway and immunotherapies to name but a few, with several clinical trials underway. It is likely that the heterogeneous nature of pancreatic cancer necessitates a more personalised approach to management with targeted treatment guided by predictive biomarkers.
Thillai K, Sarker D, Ross P. Progress in pancreatic cancer therapeutics: The potential to exploit molecular targets. World J Pharmacol 2015; In press
INTRODUCTION
Pancreatic ductal adenocarcinoma (PDAC) is the 5th leading cause of cancer related mortality worldwide. Despite significant research efforts, 5-year survival for all stages remains stagnant at 3%-5%[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_1" \o "who.int, #1" 1, HYPERLINK \l "_ENREF_2" \o "Ryan, 2014 #2" 2]. More than 80% of patients present with inoperable or advanced disease, so there remains an urgent need for more effective systemic treatments[ HYPERLINK \l "_ENREF_3" \o "Siegel, 2013 #3" ADDIN EN.CITE Siegel201333317Siegel, R.Naishadham, D.Jemal, A.Surveillance Information, Surveillance and Health Services Research, American Cancer Society, Atlanta, GA 30303-1002, USA. Rebecca.siegel@cancer.orgCancer statistics, 2013CA: a cancer journal for cliniciansCA Cancer J ClinCA: a cancer journal for cliniciansCA Cancer J ClinCA: a cancer journal for cliniciansCA Cancer J Clin11-306312013/01/22American Cancer SocietyFemaleHumansIncidenceMaleMorbidity/trendsNeoplasms/*epidemiologyRegistriesSurvival Rate/trendsUnited States/epidemiology2013Jan1542-4863 (Electronic)
0007-9235 (Linking)23335087http://www.ncbi.nlm.nih.gov/pubmed/2333508710.3322/caac.21166eng3]. Although newer combination chemotherapy regimens offer improved survival outcomes, therapeutic options are limited and do not fully exploit the unique biology of the disease. To date no predictive or prognostic biomarker has been validated for use. This review will discuss current management and emerging therapeutic concepts.
THE MOLECULAR BIOLOGY OF PDAC
Pancreatic cancer is a genetic disease, the biology of which is both intricate and highly heterogeneous. Several extensive genomic studies have confirmed that the development of PDAC results from several complex genetic aberrations with mutations in both oncogenes and tumour suppressor genes[ HYPERLINK \l "_ENREF_4" \o "Hidalgo, 2012 #4" ADDIN EN.CITE Hidalgo201244417Hidalgo, M.Centro Nacional de Investigaciones Oncologicas, Madrid, Spain. mhidalgo@cnio.esNew insights into pancreatic cancer biologyAnn OncolAnnals of oncology : official journal of the European Society for Medical Oncology / ESMOAnn OncolAnnals of oncology : official journal of the European Society for Medical Oncology / ESMOAnn OncolAnnals of oncology : official journal of the European Society for Medical Oncology / ESMOx135-823 Suppl 102012/09/26*Carcinoma, Pancreatic Ductal/genetics/pathologyHumansNeoplastic Stem CellsPancreas/metabolism/pathology*Pancreatic Neoplasms/genetics/pathologyTumor Microenvironment2012Sep1569-8041 (Electronic)
0923-7534 (Linking)22987949Congresses
Overall
Research Support, Non-U.S. Gov'thttp://www.ncbi.nlm.nih.gov/pubmed/2298794910.1093/annonc/mds3134]. The progression from the pre-malignant dysplastic cellular transformations to the final development of PDAC is associated with increasing mutational changes, confirming the importance of such genetic variations in cancer development. Nearly all pancreatic cancers harbour a KRAS mutation and the majority are also associated with inactivation of CDKN2a/INK4a, TP53 and DPC4/SMaD4[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_4" \o "Hidalgo, 2012 #4" 4- HYPERLINK \l "_ENREF_7" \o "Jones, 2008 #7" 7]. Several groups have aimed to accurately depict the genomics of PDAC with each reporting numerous activating mutations. Importantly, there was heterogeneity amongst the different pancreatic tumours with several key pathways responsible for cancer progression differing between patient samples[ HYPERLINK \l "_ENREF_7" \o "Jones, 2008 #7" ADDIN EN.CITE ADDIN EN.CITE.DATA 7]. Clonal mutations found in metastatic lesions were identified in primary cancers but due to the unstable genetic nature of PDAC, these initial mutations continued to evolve resulting in heterogeneity amongst the different metastatic deposits in the same patient. This complex genetic landscape results in an aggressive pathology, often refractory to treatment resulting in poor survival outcomes.
A recent addition to the understanding of PDAC comes from recent studies that have identified a group of pancreatic cancer cells that display stem cell properties[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_4" \o "Hidalgo, 2012 #4" 4, HYPERLINK \l "_ENREF_8" \o "Hermann, 2007 #8" 8]. These cells appear to have the ability of self-renewal and asymmetric division. Preliminary data suggests that patients with tumours containing cancer stem cells (CSCs) are associated with poorer overall survival (OS). The identification of CSCs and the signalling pathways that they regulate, has led to newer therapeutic targets such as WnT, Hedgehog and Notch. Further research is needed to see whether these can be successfully exploited to produce meaningful clinical outcomes.
Despite promising pre-clinical studies, several chemotherapeutics and targeted agents have failed to reproduce positive results in patients (Table 1). One explanation for this relates to the complex micro-tumour environment that surrounds the cancer cells and the difficulties replicating this in-vitro. A significant bulk of the pancreatic tumour comprises not of malignant cells but of the encompassing dense fibrotic stromal matrix[ HYPERLINK \l "_ENREF_9" \o "Mahadevan, 2007 #9" ADDIN EN.CITE Mahadevan200799917Mahadevan, D.Von Hoff, D. D.Hematology/Oncology, The University of Arizona Cancer Center, 1515 North Campbell Avenue, Tucson, AZ 58724, USA. dmahadevan@azcc.arizona.eduTumor-stroma interactions in pancreatic ductal adenocarcinomaMol Cancer TherMolecular cancer therapeuticsMol Cancer TherMolecular cancer therapeuticsMol Cancer TherMolecular cancer therapeutics1186-97642007/04/05AnimalsCarcinoma, Pancreatic Ductal/genetics/*pathology/therapy*Cell CommunicationDisease Models, AnimalGene Expression ProfilingHumansPancreatic Neoplasms/genetics/*pathology/therapyStromal Cells/*pathology2007Apr1535-7163 (Print)
1535-7163 (Linking)17406031Research Support, N.I.H., Extramural
Reviewhttp://www.ncbi.nlm.nih.gov/pubmed/1740603110.1158/1535-7163.MCT-06-0686eng9]. This micro-tumour environment results from the extensive desmoplastic reaction seen in PDAC and consists of an abundant extracellular matrix, pancreatic stellate cells, fibroblasts, immune cells, inflammatory cells and vasculature all of which were previously thought to aid proliferation, invasion and metastatic spread whilst also preventing adequate drug delivery leading to chemotherapy-resistance[10-14]. The success of nab-paclitaxel (as discussed later) appear to manipulate the distinct characteristics of the stroma for therapeutic benefit[15].
However recent emerging research using genetically modified mouse models suggest that depletion of the stroma (by genetic or pharmacological targeting of the Hedgehog HYPERLINK \l "_ENREF_16" \o "Jesus-Acosta, 2014 #99" ADDIN EN.CITE Jesus-Acosta201499999917De Jesus-AcostaA phase II study of vimodegib, a hedgehog (Hh) pathway inhibitor, combined with gemciatbine and nab-paclitaxel (nab-P) in patients (pts) with untreated metastatic pancreatic ductal adenocarcinoma (PDA)J clinic oncol 32, 2014 (suppl 3; abstr 257)2014[16] pathway) results in an unexpected increase in tumour vascularity and proliferation, thereby resulting in more aggressive tumours with reduced survival[ HYPER L I N K \ l " _ E N R E F _ 1 7 " \ o " R h i m , 2 0 1 4 # 1 " A D D I N E N . C I T E A D D I N E N . C I T E . D A T A 1 7 ] . F u r t h e r m o r e , t r a n s g e n i c m i c e w i t h t h e a b i l i t y t o d e l e t e S M A + m y o f i b r o b l a s t s i n p a n c r e a t i c c a n c e r a l s o d e m o n s t r a t e d r e d u c e d s u r v i v a l [ H Y P E R L I N K \ l " _ E N R E F _ 1 8 " \ o " O z d emir, 2014 #17" ADDIN EN.CITE ADDIN EN.CITE.DATA 18]. Both studies suggest that rather than a promoter of cancer growth, the stroma (or at least part of it) may paradoxically act to suppress proliferation and angiogenesis thus targeting the stroma should be performed with caution. An intricate and crucial part of PDAC, further research into the stroma is needed in order to exploit its presence for therapeutic benefit.
CHEMOTHERAPY
Until recently the standard treatment for inoperable or metastatic disease was with the nucleoside analogue gemcitabine. This was based on the results of a phase III trial in 1997 of 126 patients with advanced PDAC. Patients were randomised to receive gemcitabine(gemcitabine 1000 mg/m2 weekly 7 followed by 1 wk of rest, then weekly 3 every 4 wk thereafter), or to fluorouracil (5-FU) (600 mg/m2 once weekly)[ HYPERLINK \l "_ENREF_19" \o "Burris, 1997 #18" ADDIN EN.CITE ADDIN EN.CITE.DATA 19]. Both arms continued treatment until progression or unacceptable toxicities and the primary end point was clinical benefit, measured using a combined score of pain, performance status (PS) and weight loss. Clinical benefit response was experienced by 23.8% of gemcitabine-treated patients compared with 4.8% of 5-FU-treated patients (P = 0.0022). There was also a modest survival benefit with gemcitabine with a 12-mo survival of 18% vs 2% (P = 0.0025) with a median OS of 5.6 m for patients treated with gemcitabine and 4.4 mo for those with 5FU. Treatment was well tolerated and gemcitabine became standard treatment for inoperable and advanced disease.
Numerous clinical trials with various chemotherapy agents combined with gemcitabine ensued (Table 2) and following several disappointing outcomes, the majority of preclinical work focused on developing new targeted treatments. However, the most significant advances in PDAC management were with two new chemotherapy combinations that have recently demonstrated benefits over gemcitabine in large phase III trials and thus changed current practise. The combined treatment of oxaliplatin, irinotecan, leucovorin and fluorouracil (FOLFIRINOX) was associated with a median overall survival (OS) of 11.1 mo compared with 6.8 mo in patients treated with gemcitabine alone (HR for death 0.57 CI: 0.45-0.73, P d" 0 . 0 0 1 ) [ A D D I N E N . C I T E A D D I N E N . C I T E . D A T A H Y P E R L I N K \ l " _ E N R E F _ 2 0 " \ o " C o n r o y , 2 0 1 1 # 1 9 " 2 0 , H Y P E R L I N K \ l " _ E N R E F _ 2 1 " \ o " P a p a d a t o s - P a s t o s , 2 0 1 4 # 2 0 " 2 1 ] . T h i s p h a s e I I I t r i a l o f 3 4 2 p a t i e n t s w i t h P S 0 o r 1 a l s o d e m o n s t r a t e d i n c r e a s e s i n m e d i a n p r o g r e s s i o n f r e e s u r v i v a l ( P F S ) ( 6 . 4 m v s 3 . 3 m , P d" 0 . 0 0 1 ) a n d o b j e c t i v e r e s p o n s e r a t e ( O R R ) ( 3 1 . 6 v s 9 . 4 , P d" 0 . 0 0 1 ) ) . A p p r o v e d f o r u s e i n f i r s t l i n e m e t a s t a t i c d i s e a s e , i n p r a c t i s e t h i s r e g i m e n i s g e n e r a l l y r e s e r v e d f o r p a t i e n t s w i t h a n e x c e l l e n t performance status as unsurprisingly, toxicity was also significantly increased with this 3-drug combination. More recently a phase III trial compared combined nab-paclitaxel and gemcitabine with gemcitabine alone in patients with metastatic disease[ HYPERLINK \l "_ENREF_22" \o "Von Hoff, 2013 #21" ADDIN EN.CITE ADDIN EN.CITE.DATA 22]. Median OS was 8.5 mo with the combination chemotherapy and 6.7 mo with gemcitabine (95%CI: 0.62 to 0.83, P < 0.001). ORR was significantly increased at 23% vs 7% (P < 0.001), leading to interest in the potential use as a means of down staging locally advanced disease. A further pre-specified sub-group analysis concluded that baseline Karnofsky score (KS), presence of liver metastases, age and number of metastatic sites were independent prognostic factors for OS and PFS[ HYPERLINK \l "_ENREF_23" \o "Tabernero, 2015 #2" ADDIN EN.CITE ADDIN EN.CITE.DATA 23]. Common adverse events of grade 3 or higher included neutropenia (38% in nab-paclitaxel and gemcitabine arm and 27% in the gemcitabine arm), fatigue (17% and 7%) and neuropathy (17% and 1%). Nab-paclitaxel is a colloidal suspension of 130nm particles homogenised in human serum albumin that is bound to paclitaxel. Pancreatic cancers are known to overexpress SPARC (Secreted protein acidic and rich in cysteine) and nab-paclitaxel improves efficacy via SPARC-albumin binding[ HYPERLINK \l "_ENREF_24" \o "Arnold, 2010 #23" ADDIN EN.CITE ADDIN EN.CITE.DATA 24]. Pre-clinical models have confirmed that SPARC overexpression in the stroma promotes cell invasion and metastatic spread. Higher levels of SPARC appeared to correlate with improved survival in the original phase I/II trial of gemcitabine and nab-paclitaxel (mOS was 17.8 m in the high SPARC group compared with 8.1 m in the low SPARC group, P = 0.431). Further research is needed to confirm whether SPARC has the potential to be used as a predictive marker. The recently reported results from a prospective randomised adjuvant study have also suggested the prognostic significance of over-expressed SPARC in patients undergoing resection with curative intent. Disease free survival (DFS) was 7.4 mo in patients with higher levels of SPARC compared to 12.1 m in those with lower levels (P = 0.041) and OS was 14.1 and 25.6 m respectively (P = 0.011)[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_25" \o "al, 2013 #24" 25, HYPERLINK \l "_ENREF_26" \o "Von Hoff, 2011 #25" 26]. Without a direct head- to head trial of both combination chemotherapy regimens, it is difficult to ascertain whether FOLFIRINOX or gemcitabine and nab-paclitaxel is superior and both are now standard practise. However single agent gemcitabine remains treatment of choice for those patients that are not suitable for combination therapy.
RAS-RAF-MEK PATHWAY
Approximately 30% of all patients with solid malignancies have tumours that exhibit oncogenic Ras mutations[ HYPERLINK \l "_ENREF_27" \o "Kanda, 2012 #26" ADDIN EN.CITE ADDIN EN.CITE.DATA 27]. In PDAC this figure is much higher as an excess of 95% have a small GTPase KRAS mutation resulting in a dominant activated form. These mutations cause the protein to be constitutively activated, which leads to aberrant down-stream signalling and increased proliferation[ HYPERLINK \l "_ENREF_28" \o "Hruban, 1993 #27" ADDIN EN.CITE ADDIN EN.CITE.DATA 28]. Following the discovery of the Ras family, a concerted effort was made to develop agents that could block mutated Ras function with little success.
As KRAS requires binding to the plasma membrane via farnesylation or geranylgeranlyation in order to become activated, several farnesyltransferase inhibitors (FFIs) have been developed but have proved ineffective in clinical trials. Two phase II trials using FFIs were negativeand a randomised doubled blind phase III trial of 688 patients comparing gemcitabine with or without the FFI tipifarnib, demonstrated no significant survival benefit in the combination arm compared with standard treatment[29-31]. A further stu d y d e m o n s t r a t e d t h a t b i n d i n g o f m a m m a l i a n P D E t o K R A S u s i n g s m a l l m o l e c u l e i n h i b i t o r s c a n s u p p r e s s o n c o g e n i c R A S s i g n a l l i n g b y v i r t u e o f s e l e c t i v e b i n d i n g t o t h e p r e n y l - b i n d i n g p o c k e t o f P D E a n d i n P D A C c e l l l i n e s r e s u l t e d i n r e d u c e d c e l l p r o l i f e r a t i o n [ HYPERLINK \l "_ENREF_32" \o "Zimmermann, 2013 #31" ADDIN EN.CITE ADDIN EN.CITE.DATA 32]. Other approaches include the development of small molecules that target son of sevenless (SOS) mediated nucleotide exchange and subsequently target KRAS[ HYPERLINK \l "_ENREF_33" \o "Maurer, 2012 #32" ADDIN EN.CITE ADDIN EN.CITE.DATA 33] and recently KRASG12C inhibitors have demonstrated therapeutic potential by allosterically allowing KRAS to favour GDP over GTP[ HYPERLINK \l "_ENREF_34" \o "Ostrem, 2013 #33" ADDIN EN.CITE ADDIN EN.CITE.DATA 34]. Another recent approach to targeting KRAS is by the combined MEK/BCL-XL inhibition, a method developed after identification in a pooled shRNA screen[ HYPERLINK \l "_ENREF_35" \o "Corcoran, 2013 #34" ADDIN EN.CITE ADDIN EN.CITE.DATA 35]. This combination resulted in significant apoptosis in several KRAS mutated cell lines.
A recent pre-clinical study demonstrated a novel way of targeting KRAS in transgenic mouse models using an siRNA delivery system (Local drug EluteR or LODER)[ HYPERLINK \l "_ENREF_36" \o "Zorde Khvalevsky, 2013 #35" ADDIN EN.CITE ADDIN EN.CITE.DATA 36]. This model capitalises on the effects of siRNA and knockdown of KRAS, but via an innovative platform of a controlled and prolonged delivery for therapeutic benefit. The LODER against KRAS (siG12D LODER) decreased KRAS levels in pancreatic cancer cell lines resulting in reduced proliferation and epidermal-mesenchymal transition. Within in-vivo models, the growth of human pancreatic cancer cells was impaired and mouse survival was increased compared to controls. A phase 1 study in patients with locally advanced disease treated with siG12D LODER is on-going[ HYPERLINK \l "_ENREF_37" \o "NCT01188785, #36" ADDIN EN.CITE NCT0118878536363617NCT01188785Phase 1 - Escalating dose study of siG12D LODER (Local Drug EluteR) in patients with advanced adenocarcinoma of the pancreas and a single dose study of siG12D LODER (Local drug EluteR) in patients with operable adenocarcinoma of the pancreasclinicaltrials.govclinicaltrials.gov37], and a further phase II study of siG12D LODER in combination with chemotherapy plans to open early next year[ HYPERLINK \l "_ENREF_38" \o "NCT01676259, #37" ADDIN EN.CITE NCT0167625937373717NCT01676259A phase II study of siG12D LODER in combination with chemotherapy in paitents with unresectable locally advanced pancreatic cancerclinicaltrials.govclinicaltrials.gov38]. Whilst these results are promising in the pursuit of an anti-KRAS therapy, it remains to be seen whether this can be translated in to an efficacious treatment in clinical trials.
With the limited success of inhibiting Ras, efforts have moved towards targeting downstream signalling activity. There are two main pathways that have been extensively interrogated, MAPK and PI3K signalling (Figure 1). BRaf inhibitors, such as vemurafenib, work downstream from Ras and have had considerable success in Raf mutant tumours such as melanoma[ HYPERLINK \l "_ENREF_39" \o "Chapman, 2011 #38" ADDIN EN.CITE ADDIN EN.CITE.DATA 39]. However, evidence now supports that there is a paradoxical up regulation of MAPK signalling when Raf is inhibited in KRAS mutated tumours[ HYPERLINK \l "_ENREF_40" \o "Hatzivassiliou, 2010 #39" ADDIN EN.CITE ADDIN EN.CITE.DATA 40]. In pancreatic cancer where Raf is wild type and Ras is nearly always mutated, Raf inhibitors create feedback activation of the MAPK signalling pathway therefore it is likely that targeting downstream by MEK inhibition will offer more promising results. Phase I/II clinical trials of various MEK inhibitors in combination with gemcitabine are currently underway following positive pre clinical work[ HYPERLINK \l "_ENREF_41" \o "NCT01231581, #40" ADDIN EN.CITE NCT0123158140404017NCT01231581Study of GSK1120212 plus gemcitabine vs placebo plus gemcitabine in metastatic pancreatic cancerclinicaltrials.govclinicaltrials.gov41, HYPERLINK \l "_ENREF_42" \o "NCT01016483, #41" ADDIN EN.CITE NCT0101648341414117NCT01016483Trial of gemcitabine with or without MSC1936369B in pancreatic cancerclinicaltrials.govclinicaltrials.gov42], Results from a phase IIa trial of 60 patients treated with gemcitabine in combination with the allosteric oral Mek1/2 inhibitor refametinib were presented at ASCO 2104. The best result was partial response in 35% of patients with median duration of response at 3.8 mo (117 days 95%CI: 83-265). Time to progression was 7.4 mo[ ADDIN EN.CITE NCT0125164042424217NCT01251640Combination with gemcitabine in advanced pancreatic cancer (BAGPAC)clinicaltrials.govclinicaltrials.govJ201443434317Van Laethem JPhase II study of the MEK inhibitor refametinib (BAY 86-9766) in combination with gemcitabine in patietns with unresectable, locally advacned, or metastatic pancreatic cancer2014 ASCO annual meeting2014 ASCO annual meeting4025J clin Oncol 32/5s, 2014 (suppl; abst 4025)2014 HYPERLINK \l "_ENREF_43" \o "NCT01251640, #42" 43, HYPERLINK \l "_ENREF_44" \o "J, 2014 #43" 44]. KRAS mutations were identified in 39 patients (65%) and the results suggested a trend towards improved survival outcomes in patients with KRAS wild type tumours. The OS for the KRAS mutant subgroup was 6.6 mo compared with 18.2 mo (HR 0.27).
PI3K-MTOR PATHWAY
Phosphoinositide 3 kinase (PI3K) is an enzyme that lies downstream from RAS and is responsible for the activation of AKT, which in turn leads to activation of mammalian target of rapamycin (mTOR) (Figure 1). In normal tissue the PI3K-AKT pathway inhibits apoptosis and cell proliferation, thus deregulation of this pathway leads to unregulated cell[ HYPERLINK \l "_ENREF_45" \o "Falasca, 2011 #44" ADDIN EN.CITE ADDIN EN.CITE.DATA 45]. Several PI3K inhibitors have been developed and are currently being investigated in a number of malignancies. Preliminary studies using transgenic mice have demonstrated reduced pancreatic tumour growth when PI3K is inhibited and therefore PI3K remains a valid therapeutic target that warrants further attention[ HYPERLINK \l "_ENREF_46" \o "Eser, 2013 #45" ADDIN EN.CITE ADDIN EN.CITE.DATA 46]. The dual PI3K/PDK inhibitor rigosertib, has demonstrated safety and some efficacy when combined with gemcitabine in pre-treated patients with advanced disease[ HYPERLINK \l "_ENREF_47" \o "Ma, 2012 #46" ADDIN EN.CITE ADDIN EN.CITE.DATA 47] and a phase III trial in combination with chemotherapy is underway[ HYPERLINK \l "_ENREF_48" \o "NCT01360853, #47" ADDIN EN.CITE NCT0136085347474717NCT01360853Gemcitabine and ON 01910.Na in previously untreated metasatic pancreatic cancer (ONTRAC)clinicaltrials.govclinicaltrials.gov48].
mTOR has been identified as a critical effector in cell signalling and the drug everolimus, an oral inhibitor of mTOR, has had success against solid tumours such as metastatic renal cell cancers and breast cancers, but also in pancreatic neuroendocrine tumours[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_49" \o "Motzer, 2008 #48" 49- HYPERLINK \l "_ENREF_51" \o "Yao, 2011 #50" 51]. Preclinical studies showed that inhibition of the mTOR pathway suppressed proliferation in pancreatic cancer cell lines[ HYPERLINK \l "_ENREF_52" \o "Matsubara, 2013 #51" ADDIN EN.CITE Matsubara201351515117Matsubara, S.Ding, Q.Miyazaki, Y.Kuwahata, T.Tsukasa, K.Takao, S.Cancer and Regenerative Medicine, Frontier Biomedical Science and Swine Research Center, Sakuragaoka, Kagoshima, 890-8520, Japan.mTOR plays critical roles in pancreatic cancer stem cells through specific and stemness-related functionsSci RepScientific reportsSci RepScientific reportsSci RepScientific reports323032013/11/1620132045-2322 (Electronic)
2045-2322 (Linking)24231729Research Support, Non-U.S. Gov'thttp://www.ncbi.nlm.nih.gov/pubmed/24231729382857210.1038/srep03230eng52]. However in a phase II study of 33 patients with gemcitabine refractory metastatic PDAC, there were no complete or partial responses and median PFS was 1.8 mo and OS was 4.5 mo[ HYPERLINK \l "_ENREF_53" \o "Wolpin, 2009 #52" ADDIN EN.CITE ADDIN EN.CITE.DATA 53]. A phase 1 trial combining gemcitabine with temsirolimus, another mTOR inhibitor, resulted in significant toxicity without any partial or complete responses. The commonly used anti-diabetic drug metformin is also known to inhibit mTOR and epidemiological studies have linked metformin use with reduced risk of developing malignancies[ HYPERLINK \l "_ENREF_54" \o "Dowling, 2007 #53" ADDIN EN.CITE ADDIN EN.CITE.DATA 54, HYPERLINK \l "_ENREF_55" \o "Evans, 2005 #54" ADDIN EN.CITE Evans200554545417Evans, J. M.Donnelly, L. A.Emslie-Smith, A. M.Alessi, D. R.Morris, A. D.Division of Community Health Sciences, Section of Public Health, University of Dundee, Dundee DD2 4BF. j.m.m.stansfield@dundee.ac.ukMetformin and reduced risk of cancer in diabetic patientsBMJBmjBMJBmjBMJBmj1304-533075032005/04/26AgedCase-Control StudiesDiabetes Mellitus, Type 2/*drug therapyFemaleHumansHypoglycemic Agents/*therapeutic useMaleMetformin/*therapeutic useNeoplasms/*prevention & controlPilot ProjectsRisk Factors2005Jun 41756-1833 (Electronic)
0959-535X (Linking)15849206Research Support, Non-U.S. Gov'thttp://www.ncbi.nlm.nih.gov/pubmed/1584920655820510.1136/bmj.38415.708634.F755]. Metformin is now the focus of a clinical trial and is being used in combination with chemotherapy[ HYPERLINK \l "_ENREF_56" \o "NCT01210911, #55" ADDIN EN.CITE NCT0121091155555517NCT01210911Metformin combined with chemotherapy for pancreatic cancer(GEM)clinicaltrials.govclinicaltrials.gov56]. Novel agents that comprise of both mTOR complex 1 and 2 inhibitors (mTORC1/2) have shown promising efficacy in cancer cells in vitro. One such agent, INK-128, led to pancreatic cancer cell apoptosis and necrosis in vitro. Furthermore INK-128 resulted in increased sensitivity of pancreatic cancer cells to gemcitabine suggesting potential benefit when used in combination with chemotherapy[ HYPERLINK \l "_ENREF_57" \o "Lou, 2014 #56" ADDIN EN.CITE ADDIN EN.CITE.DATA 57]. Other positive resu l t s h a v e b e e n r e p o r t e d w i t h d u a l P I 3 K / m T O R k i n a s e i n h i b i t o r s i n v i t r o [ H Y P E R L I N K \ l " _ E N R E F _ 5 8 " \ o " T a n g , 2 0 1 4 # 5 7 " A D D I N E N . C I T E A D D I N E N . C I T E . D A T A 5 8 ] . R e c e n t l y i n h i b i t o r s a g a i n s t t h e p 1 1 0 i s o f o r m o f P I 3 K d e m o n s t r a t e d i n a c t i v a t i o n o f r e g u l a tory T cells leading to CD8+ cytotoxic cells and subsequent tumour regression in murine models[ HYPERLINK \l "_ENREF_59" \o "Ali, 2014 #58" ADDIN EN.CITE ADDIN EN.CITE.DATA 59]. Despite limited results in clinical trials thus far, recent pre-clinical efforts are more promising and the PI3K/AKT/mTOR signalling cascade remains an important pathway for future research.
EPIDERMAL GROWTH FACTOR RECEPTOR
The epidermal growth factor receptor (EGFR) has also emerged as an attractive therapeutic target for many malignancies. EGFR is a member of the erbB/human EGFR family of tyrosine kinases and when bound to a ligand, a conformational change is induced leading to dimerisation with other receptors[ HYPERLINK \l "_ENREF_60" \o "Zhang, 2007 #59" ADDIN EN.CITE ADDIN EN.CITE.DATA 60]. This results in the activation of several cascades including the Ras/MAP kinase pathway and the PI3K/Akt/mTOR pathway. Several small molecules have been developed that block EGFR with varying degrees of success. The only targeted drug to be approved for the management of advanced PDAC so far is the tyrosine kinase inhibitor (TKI) erlotinib when administered in combination with gemcitabine. A phase III trial (PA.3) randomly assigned 569 patients with advanced disease to receive standard gemcitabine plus erlotinib (100 or 150 mg/d orally) or placebo[ HYPERLINK \l "_ENREF_61" \o "Moore, 2007 #60" ADDIN EN.CITE ADDIN EN.CITE.DATA 61]. The trial was double blinded with a primary end point of overall survival. The r e s u l t s s h o w e d a m o d e s t b u t s i g n i f i c a n t s u r v i v a l b e n e f i t i n t h e c o m b i n a t i o n a r m ( 6 . 2 4 m v s 5 . 9 1 m H R , 0 . 8 2 9 5 % C I : 0 . 6 9 - 0 . 9 9 , P d" 0 . 0 3 8 ) , w h i c h l e d t o F D A a p p r o v a l . A l t h o u g h t h e b e n e f i t s a p p e a r e d t o b e s m a l l , a n u n p l a n n e d r e t r o s p e c t i v e s u b g r o u p a n a l y s i s l ed the authors to hypothesise that patients who developed a skin rash on treatment experienced a higher disease control rate. Patients that were younger than 65 (P = 0.1) and those with a good PS (P = 0.03) were more likely to develop a rash. The median OS in patients with a grade 0, 1 or 2 rash were 5.3 m, 5.8 m and 10.5 m respectively with 1 year survival rates of 16%, 9% and 43% (P < 0.001). This was further assessed in a study correlating rash and survival outcomes, by analysing combined data from the PA.3 trial and a phase III trial using erlotinib in advanced non-small cell lung cancer (BR.21)[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_62" \o "Wacker, 2007 #61" 62, HYPERLINK \l "_ENREF_63" \o "Shepherd, 2005 #62" 63]. They found that the presence of grade 2 or higher rash correlated with improvements in PFS and disease control. These findings were echoed in a retrospective study of 174 patients that found that high-severity rash was associated with longer OS[ HYPERLINK \l "_ENREF_64" \o "Stepanski, 2013 #63" ADDIN EN.CITE ADDIN EN.CITE.DATA 64].
However, molecular studies have not been able to identify EGFR and KRAS mutations as predictive biomarkers of survival benefit and no association between KRAS mutation or EGFR gene copy number with rash has been identified. Erlotinib in combination with capecitabine has also been shown to have some activity in gemcitabine refractory patients as evidenced by a phase II trial combining capecitabine and erlotinib in patients with advanced PDAC. The primary end point was response and this was found to be 10% of all 30 patients with a median OS of 6.5 mo[ HYPERLINK \l "_ENREF_65" \o "Kulke, 2007 #64" ADDIN EN.CITE ADDIN EN.CITE.DATA 65]. A further phase III trial comparing combined capecitabine and erlotinib followed by gemcitabine on progression compared with gemcitabine and erlotinib followed by capecitabine is on-going[ HYPERLINK \l "_ENREF_66" \o "NCT00440167, #65" ADDIN EN.CITE NCT0044016765656517NCT00440167Capecitabine/erlotinib followed of gemcitabine versus gemcitabine/erlotinib followed of capecitabineclinicaltrials.govclinicaltrials.gov66].
An alternative anti EGFR TKI, gefitinib, demonstrated anti-proliferative effects in the pre clinical setting and this has translated to positive survival benefit in patients with non small cell EGFR mutated lung cancer[ HYPERLINK \l "_ENREF_67" \o "Zhou, 2009 #66" ADDIN EN.CITE ADDIN EN.CITE.DATA 67]. Gefitinib combined with gemcitabine has been assessed in a phase II trial of 53 patients with locally advanced or metastatic PDAC. Patients were treated with gefitinib (250 mg) once daily and gemcitabine at the standard dose and schedule. 6 mo PFS was 30% with a median PFS of 4.1 mo. The 1-year survival rate was measured at 7.3 mo. Whilst these results were comparable to the PA 3 trial, there has yet to be a randomised trial of gefitinib to demonstrate significant benefit over single agent gemcitabine[ HYPERLINK \l "_ENREF_68" \o "Fountzilas, 2008 #67" ADDIN EN.CITE ADDIN EN.CITE.DATA 68].
The anti-EGFR antibody cetuximab has shown significant clinical activity in both colorectal cancers and head and neck tumours in patients with wild type KRAS[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_69" \o "Bonner, 2010 #68" 69, HYPERLINK \l "_ENREF_70" \o "Van Cutsem, 2011 #69" 70]. Despite the majority of patients with pancreatic cancer having KRAS mutations, preclinical activity suggested that it might be a useful therapy in advanced PDAC due to EGFR overexpression[ HYPERLINK \l "_ENREF_71" \o "Bruns, 2000 #70" ADDIN EN.CITE ADDIN EN.CITE.DATA 71-73]. A phase II trial evaluated gemcitabine and cetuximab in 41 treatment-nave patients stratified according to EGFR expression using immunohistochemistry (4 patients were 1+, 20 patients were 2+ and 17 patient were 3+)[ HYPERLINK \l "_ENREF_74" \o "Xiong, 2004 #73" ADDIN EN.CITE ADDIN EN.CITE.DATA 74]. Cetuximab was administered at a loading dose of 400 mg/m2 followed by 250 mg/m2 weekly and gemcitabine was administered 1000 mg/m2 weekly for 7 wk and then 100 mg/m2 every week for three weeks followed by a weeks rest. Five patients achieved a partial response (12.5%) and 26 patients (63.4%) had disease stability. Median TTP was 3.8 mo and the median OS was 7.1 mo. Survival at 1 year was 31.7%. Toxicities were as previously reported with cetuximab chemotherapy combinations, most notably rash (87.7%), nausea (61.0%), weight loss (58.5%) and diarrhoea (53.7%). Despite the promising results from this phase II trial, this was not reproduced in 2 phase III trials. The S0205 trial conducted by the southwest oncology group (SWOG) reported that in 766 patients treated with either gemcitabine or gemcitabine plus cetuximab, there was no survival benefit seen in the combination arm[ HYPERLINK \l "_ENREF_75" \o "Philip, 2010 #74" ADDIN EN.CITE ADDIN EN.CITE.DATA 75]. A further trial combining gemcitabine and cisplatin with or without the addition of cetuximab, recruited 40 patients. Seven patients had a documented response in the antibody arm compared to 5 in the control arm but again no survival benefit was seen with cetuximab[ HYPERLINK \l "_ENREF_76" \o "Cascinu, 2008 #75" ADDIN EN.CITE ADDIN EN.CITE.DATA 76]. A further negative phase II trial with gemcitabine and oxaliplatin with the addition of cetuximab recruited 64 patients. Patients received a combination of gemcitabine at 100 mg/m2 on day 1 with oxaliplatin at 100 mg/m2 on day 2, every 2 wk. Cetuximab was administered at a loading dose of 400 mg/m2 followed by weekly dose of 250 mg/m2. Although well tolerated, the findings (response rate 33%, median time to PFS 3.9 mo and OS 7.1 mo) were not superior to previously seen results using the chemotherapy combination alone[ HYPERLINK \l "_ENREF_77" \o "Kullmann, 2009 #76" ADDIN EN.CITE ADDIN EN.CITE.DATA 77]. The results of a phase II trial presented at ASCO 2013, portrayed a significant survival benefit at 1 year with gemcitabine combined with the anti-EGFR antibody nimotuzumab compared to gemcitabine alone (34.4% vs 19.5%, P = 0.034, HR = 0.69) and the combination was well tolerated[ HYPERLINK \l "_ENREF_78" \o "Strumberg, 2013 #77" ADDIN EN.CITE Strumberg201377777717Dirk StrumbergPhase II, randomized, double-blind placebo-controlled trial of nimotuzumab plus gemcitabine compared with gemcitabine alone in patients (pts) with advanced pancreatic cancer (PC)J Clin Oncol 31, 2013 (suppl;abstr 4009)J Clin Oncol 31, 2013 (suppl;abstr 4009)201378]. A phase II study of nimotuzumab in pre-treated patients with advanced PDAC was also encouraging and a randomised placebo controlled phase IIb/IIIa study comparing the combination of gemcitabine and nimotuzumab compared to gemcitabine and placebo has recently closed to recruitment and the results are awaited[ HYPERLINK \l "_ENREF_78" \o "Strumberg, 2013 #77" ADDIN EN.CITE Strumberg201377777717Dirk StrumbergPhase II, randomized, double-blind placebo-controlled trial of nimotuzumab plus gemcitabine compared with gemcitabine alone in patients (pts) with advanced pancreatic cancer (PC)J Clin Oncol 31, 2013 (suppl;abstr 4009)J Clin Oncol 31, 2013 (suppl;abstr 4009)201378].
Although EGFR remains a critical receptor in pancreatic cell proliferation and metastatic spread, with the exception of the modest benefits seen in the PA3 trial, there have not been any positive results with EGFR targeted therapy in large randomised trials. Whilst monoclonal antibodies that target EGFR have demonstrated efficacy in other solid tumours, its distribution within pancreatic cancer cells is not well known and may be an explanation for poor outcomes. It is also possible that the optimum doses and methods of drug delivery have not yet been elucidated. With regards to erlotinib and gefitinib, the excellent results that have been demonstrated in several large clinical trials in lung cancer have not been reproduced in PDAC and are likely due to the lack of activating mutations seen in these tumours. There is not enough evidence to suggest that even in those with an activating mutation, this can predict response to anti-EGFR therapy. Thus disappointingly expression or mutation of EGFR has not emerged so far as a predictive or prognostic biomarker[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_79" \o "Immervoll, 2006 #78" 79- HYPERLINK \l "_ENREF_81" \o "Faller, 2009 #80" 81]. Unlike lung and colon tumours KRAS mutation is not mutually exclusive with EGFR activation. Initiation of KRAS mutated PDAC appears to be dependent on EGF activation and a recent study reported that EGF inhibition has limited therapeutic benefit in tumours with p53 inactivation[ HYPERLINK \l "_ENREF_82" \o "Navas, 2012 #81" ADDIN EN.CITE ADDIN EN.CITE.DATA 82]. The study hypothesised that p53 loss might reactivate the PI3K/AKT and the STAT pathway independent from EGF activation suggesting EGFR inhibitors may only be of clinical benefit in patients with p53 wild type tumours.
ANGIOGENIC THERAPY
Angiogenesis describes the process by which a tumour initiates the formation of new vessels through remodelling of existing vasculature[ HYPERLINK \l "_ENREF_83" \o "Hanahan, 1996 #82" ADDIN EN.CITE Hanahan199682828217Hanahan, D.Folkman, J.Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0534, USA.Patterns and emerging mechanisms of the angiogenic switch during tumorigenesisCellCellCellCellCellCell353-648631996/08/09AnimalsApoptosisGrowth Inhibitors/physiologyHumansNeoplasms/*blood supplyNeovascularization, Pathologic/*physiopathology1996Aug 90092-8674 (Print)
0092-8674 (Linking)8756718Research Support, U.S. Gov't, P.H.S.
Reviewhttp://www.ncbi.nlm.nih.gov/pubmed/8756718eng83]. Once the angiogenic switch is initiated, the complex process of new vessel formation begins and subsequently plays a key role in tumour growth[ HYPERLINK \l "_ENREF_84" \o "Bergers, 2003 #83" ADDIN EN.CITE Bergers200383838317Bergers, G.Benjamin, L. E.University of California San Francisco, Department of Neurological Surgery, Brain Tumor Research Center and Comprehensive Cancer Center, HSE 722, 513 Parnassus Avenue, San Francisco, California 94143-0520, USA. bergers@cgl.ucsf.eduTumorigenesis and the angiogenic switchNat Rev CancerNature reviews. CancerNat Rev CancerNature reviews. CancerNat Rev CancerNature reviews. Cancer401-10362003/06/05AnimalsDisease ProgressionHumansNeoplasms/*blood supplyNeovascularization, Pathologic/*metabolism/prevention & control2003Jun1474-175X (Print)
1474-175X (Linking)12778130Reviewhttp://www.ncbi.nlm.nih.gov/pubmed/1277813010.1038/nrc109384]. VEGF is vital to angiogenesis and is therefore a potential target in many tumour types with variable outcomes in clinical trials[ HYPERLINK \l "_ENREF_85" \o "Jain, 2006 #84" ADDIN EN.CITE ADDIN EN.CITE.DATA 85]. Anti-VEGF antibodies have been used without much success in pancreatic cancer. Bevacizumab, which offers improved outcomes in colorectal and ovarian cancer, is a monoclonal antibody that decreases the formation of new blood vessels in vivo and improves drug delivery to the cancer cell. A Phase II trial of bevacizumab and gemcitabine in patients with advanced PDAC, demonstrated that in 52 patients, 19% had a partial response and 48% had stable disease[ HYPERLINK \l "_ENREF_86" \o "Kindler, 2005 #85" ADDIN EN.CITE ADDIN EN.CITE.DATA 86]. The median OS was 8.8 mo with a 6-mo survival of 77%. This led to 2 phase III trials, both of which were disappointingly negative. The CALGB 80303 study treated patients with gemcitabine with or without bevacizumab[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_87" \o "Kindler, 2010 #86" 87, HYPERLINK \l "_ENREF_88" \o "Van Cutsem, 2009 #87" 88]. 602 patients were enrolled and both overall response rate and 1 year survival outcomes failed to reach statistical benefit in the combination arm. The AViTA trial, comparing the combination of gemcitabine and erlotinib with the addition of bevacizumab was also negative[ HYPERLINK \l "_ENREF_88" \o "Van Cutsem, 2009 #87" ADDIN EN.CITE ADDIN EN.CITE.DATA 88]. Despite the changes in the vasculature seen in patients treated with these drugs, no benefit has been shown when targeting VEGF and the exact mechanism of failure remains unknown but is likely to be in part due to the hypovascularity of the surrounding stroma[ HYPERLINK \l "_ENREF_11" \o "Olive, 2009 #11" ADDIN EN.CITE ADDIN EN.CITE.DATA 11].
IMMUNOTHERAPY
Interest in immunotherapy has had a recent resurgence following the results of several positive clinical trials in solid malignancies including melanoma and prostate cancer[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_89" \o "Hodi, 2010 #88" 89, HYPERLINK \l "_ENREF_90" \o "May, 2011 #89" 90]. Success has been more modest in pancreatic cancer although several newer agents remain under investigation. Based on the understanding that the innate immune system can distinguish between cancer cells and normal self, exploitation of the immune system has been a topic of research for several decades. Not only do immune-deficient mice develop malignancies, evidence has also shown that patients with cancer develop B and T cells that can recognise antigens released by pancreatic tumour cells. The immune response created by the patient is invariably unsuccessful at eliminating malignancy but this reaction can be enhanced for therapeutic gain. Theoretically, immunotherapy should be active in pancreatic cancer, as the dense stroma is enriched with immune cells such as T cell and macrophages.
A recent positive trial presented at GI ASCO in 2014 demonstrated significant survival benefit when combining two specific anti-cancer vaccines compared with monotherapy. GVAX is a vaccine made from 2 pancreatic cancer cell lines that have been irradiated to secrete GM-CSF causing stimulation of the immune system[ HYPERLINK \l "_ENREF_91" \o "al, 2014 #90" ADDIN EN.CITE al201490909017Dung T et alA phase 2, randomized trial of GVAX pancreas and CRS-207 immunotherapy versus GVAZ alone in patients with metastatic pancreatic adenocarcinoma; Updated resultsJ Clin Oncol 32, 2014 (suppl 3;abstr 177)J Clin Oncol 32, 2014 (suppl 3;abstr 177)201491]. Administered intra-dermally after low dose cyclophosphamide, it inhibits regulatory T cells. CRS-207 is made of live-attenuated Listeria monocytogenes engineered to stimulate an immune response against a protein called mesothelin that is expressed at high levels in pancreatic cancer cell lines. This phase II trial compared the combination of CRS-207 and GVAX with GVAX alone with positive outcomes. Ninety patients with pre-treated PDAC were randomly assigned at a ratio of 2:1 to be treated with 2 dose of CY/GVAX followed by 4 doses of CRS-207 or 6 doses of CY/GVAX every three weeks. The primary end point was OS with safety, clinical response and immune response secondary. At the interim analysis median OS was 6.1 m with the combination treatment compared with 3.9m for GVAX therapy. (HR = 0.59, two sided log rank P = 0.03). 1-year survival was doubled with combination treatment (24% vs 12%). Following the encouraging results from the interim analysis, crossover was allowed. Toxicities included fevers, rigors and lymphopaenia, but were minimal and were not cumulative. Several other studies are due to open comparing combination CY/GVAX and CRS-207 with chemotherapy in the second line setting or in combination with immune checkpoint inhibitors of programmed death 1 (PD1) and its ligand PD-L1. PD1, which is a T-cell co-inhibitory receptor, and PD-L1 have shown considerable responses in certain solid tumours including melanoma and lung cancers. An international phase 1 study using the intravenous anti-PD-L1 antibody treated 75 patients, 14 of whom had pancreatic cancer. Objective responses were seen in patients with non-small lung cancer, melanoma, renal and ovarian cancer but not in those with PDAC[ HYPERLINK \l "_ENREF_92" \o "Brahmer, 2012 #91" ADDIN EN.CITE ADDIN EN.CITE.DATA 92]. However there remains potential benefit with PD-1 in combination with other compounds. Recently the effects of PD-1 immunosuppression were enhanced when used in combination with chimeric antigen reception (CAR) T-cell therapy in Her2 transgenic mice. Further research with this combination is on going. A phase 1 study combining the agonist CD40 monoclonal antibody (CP-870,893) in combination with gemcitabine demonstrated tolerability and resulted in 4 out of 22 patients with advanced PDAC achieving a partial response suggesting that further clinical trials are warranted.
The results from a phase III trial assessing the GV1001 vaccine, a promiscuous class II epitope vaccine, recently reported no benefit when used in combination with gemcitabine and capecitabine compared to chemotherapy alone and was therefore terminated early[ HYPERLINK \l "_ENREF_93" \o "Middleton, 2014 #92" ADDIN EN.CITE ADDIN EN.CITE.DATA 93]. Several other immunological treatments remain under review. The anti-CTLA4 antibody that has been approved for use in melanoma, also demonstrated no initial responders to therapy. However in this phase II trial of 27 patients, 1 patient had a significant delayed response[ HYPERLINK \l "_ENREF_94" \o "Royal, 2010 #93" ADDIN EN.CITE ADDIN EN.CITE.DATA 94]. Single agent ipilimumab has not been taken forward to a phase III trial but its safety when used in combination with gemcitabine is currently being assessed in an early phase trial[ HYPERLINK \l "_ENREF_95" \o "NCT01473940, #94" ADDIN EN.CITE NCT0147394094949417NCT01473940Ipililmumab and gemcitabine hydrochloride in treating patients with stage III-IV or recurrent pancreatic cancer that cannot be removed by surgeryclinicaltrials.govclinicaltrials.gov95]. As it has been suggested that immunotherapy is most successful in the absence of large disease burden, several clinical trials are assessing immunotherapy in the post-operative setting or as maintenance therapy following response to chemotherapy.
HEDGEHOG INHIBITORS
The hedgehog (Hh) pathway has been identified as another important signalling cascade in multiple cancers suggesting its potential as a therapeutic target. Two transmembrane proteins have been identified that activate the Hh signalling pathway, the tumour suppressor patched protein (PTCI) and smoothened (SMO) an oncogenic protein[ ADDIN EN.CITE ADDIN EN.CITE.DATA HYPERLINK \l "_ENREF_96" \o "McMahon, 2003 #95" 96- HYPERLINK \l "_ENREF_98" \o "Amakye, 2013 #97" 98]. Pre-clinical studies have established that human pancreatic stellate cells (as seen in the stroma) express high levels of smoothened protein and low levels of Hh ligands unlike the pancreatic cancer cells, which demonstrate the converse expression pattern[ HYPERLINK \l "_ENREF_11" \o "Olive, 2009 #11" ADDIN EN.CITE ADDIN EN.CITE.DATA 11]. The majority of Hh inhibitors that have been developed target SMO. In transgenic Kras mutated mice the administration of a Hh inhibitor IPI-926 depleted the surrounding stroma enhancing the drug delivery of gemcitabine[ HYPERLINK \l "_ENREF_11" \o "Olive, 2009 #11" ADDIN EN.CITE ADDIN EN.CITE.DATA 11]. A phase Ib trial of IPI-926 in combination with gemcitabine demonstrated acceptable tolerability in 16 patients with untreated metastatic PDAC. Common AEs included fatigue, thrombocytopenia, anaemia, nausea, diarrhoea, vomiting and dose reductions of IPI-926 were required in 3 patients. DR of gemcitabine occurred in 11 patients. Five sixteenths (31%) had a radiological response while median PFS was more than 7 mo with 74% patients alive after 6 mo of entry in to the study. Whilst these results were promising, a phase II trial was terminated early at the interim analysis as patients in the combination arm experienced worse outcomes than those on single agent gemcitabine[ HYPERLINK \l "_ENREF_99" \o "NCT01130142, #98" ADDIN EN.CITE NCT0113014298989817NCT01130142A study evaluating IPI-926 in combination with gemcitabine in patients with metastatic pancreatic cancerclinicaltrials.govclinicaltrials.gov99]. These disappointing results may be partly explained by the results from recent pre-clinical studies suggesting the importance of the stroma (as discussed earlier) where depletion led to increased tumour growth[16,17].
However further research with Hh inhibitors are on-going. A single-arm study with the Hh inhibitor vismodegib combined with the chemotherapy regimen gemcitabine and nab-paclitaxel, presented an interim analysis at GI ASCO 2014[ HYPERLINK \l "_ENREF_16" \o "Jesus-Acosta, 2014 #99" ADDIN EN.CITE Jesus-Acosta201499999917De Jesus-AcostaA phase II study of vimodegib, a hedgehog (Hh) pathway inhibitor, combined with gemciatbine and nab-paclitaxel (nab-P) in patients (pts) with untreated metastatic pancreatic ductal adenocarcinoma (PDA)J clinic oncol 32, 2014 (suppl 3; abstr 257)J clinic oncol 32, 2014 (suppl 3; abstr 257)201416]. Eighty percent of the 59 patients treated had stable disease or better. Median PFS was 5.5 mo and OS was 10 mo. Patient recruitment is on-going and based on the preliminary results, the final survival data is eagerly awaited.
CONCLUSION
The current prognosis for advanced PDAC remains poor, highlighting the urgent need for more effective systemic therapies. In order to develop targeted treatments and improve outcomes, research efforts needs to focus on three key areas; a greater understanding of the unique biology of PDAC and the key signalling pathways, comprehension of the unique desmoplastic reaction and micro-tumour environment, and the development of predictive and prognostic biomarkers. It may be that the future of pancreatic cancer treatment will see combining standard chemotherapy with targeted treatments to achieve better outcomes. It is likely that PDAC treatment will be dictated by the biology of the individual tumour rather than the one shoe fits all approach that is used today. The last few years have seen significant results towards this in the pre-clinical setting but it remains to be seen whether they can be translated into meaningful clinical outcomes.
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P-Reviewer: Cao DF, Fillat C, Folsch UR, Gu DS S-Editor: Ji FF L-Editor: E-Editor:
Table 1 Phase III trials with gemcitabine combination chemotherapy regimens
Date published
TargetRef.Sample size (n)TreatmentOS (mo)P value2001MMPBramhell et al[ HYPERLINK \l "_ENREF_100" \o "Bramhall, 2001 #100" ADDIN EN.CITE ADDIN EN.CITE.DATA 100]414Marimastat and gem
vs Gem5.4
5.40.992004FTVan Custem et al[ HYPERLINK \l "_ENREF_31" \o "Van Cutsem, 2004 #30" ADDIN EN.CITE ADDIN EN.CITE.DATA 31]688Tipifarnib and Gem
vs Gem
+ Placebo5.9
6.30.752009EGFRMoore et al[ HYPERLINK \l "_ENREF_61" \o "Moore, 2007 #60" ADDIN EN.CITE ADDIN EN.CITE.DATA 61]569Erlotinib and Gem
vs Gem6.2
5.90.032008EGFR/VEGFVan Cutsem et al[ HYPERLINK \l "_ENREF_88" \o "Van Cutsem, 2009 #87" ADDIN EN.CITE ADDIN EN.CITE.DATA 88]
301Gem, Erlotinib and Bevacizumab
Vs Gem, Erlotinib and Placebo7.1
6.0
0.20872010VEGFKindler et al[ HYPERLINK \l "_ENREF_87" \o "Kindler, 2010 #86" ADDIN EN.CITE ADDIN EN.CITE.DATA 87]535Gem and Bevacizumab
vs Gem and Placebo5.8
5.90.952010EGFRPhilip et al[ HYPERLINK \l "_ENREF_75" \o "Philip, 2010 #74" ADDIN EN.CITE ADDIN EN.CITE.DATA 75]745Gem vs Gem and Placebo5.9
6.40.232011VEGFKindler et al[ HYPERLINK \l "_ENREF_101" \o "Kindler, 2011 #101" ADDIN EN.CITE ADDIN EN.CITE.DATA 101]630Axitinib and Gem
vs Gem8.5
8.30.542012VEGF, BRAF, PDGFR-BGinclaves et al[ HYPERLINK \l "_ENREF_102" \o "Goncalves, 2012 #102" ADDIN EN.CITE ADDIN EN.CITE.DATA 102]104Sorafenib and Gem
vs Gem8.0
9.20.23
Gem: Gemcitabine; OS: Overall survival; MMP: Matrix metalloproteinase; FT: Farnesyl transferase; EGFR: Epidermal growth factor receptor; VEGF: Vascular endothelial growth factor; PDGFR-B: Platelet derived growth factor receptor-B.
Table 2 Phase III trials with targeted treatment in metastatic pancreatic cancer
Date publishedRegimenRef.Sample size (n)Median OS (mo)P value2001Gem vs
Gem + 5FUBerlin et al[ HYPERLINK \l "_ENREF_103" \o "Berlin, 2002 #103" ADDIN EN.CITE ADDIN EN.CITE.DATA 103]3225.4
6.70.092004Gem vs
Gem +
IrinotecanRocha et al[ HYPERLINK \l "_ENREF_104" \o "Rocha Lima, 2001 #104" ADDIN EN.CITE Rocha Lima200110410410417Rocha Lima, C. M.Sherman, C. A.Brescia, F. J.Brunson, C. Y.Green, M. R.Medical University of South Carolina, Hematology-Oncology Division, CSB 903 Charleston, SC 29525, USA. limacr@musc.eduIrinotecan/gemcitabine combination chemotherapy in pancreatic cancerOncology (Williston Park)OncologyOncology (Williston Park)OncologyOncology ( . C I Q Y Z °tZO>, #hL hO 6OJ PJ QJ nHtH hL hO OJ PJ QJ nHtH hL hO OJ QJ 2hL h t 5>*OJ PJ QJ ^J mH nHsH tH /hL hV 5OJ PJ QJ ^J mH nHsH tH#hL h@ 5OJ QJ ^J mH sH #hL hc 5OJ QJ ^J mH sH #hL hV 5OJ QJ ^J mH sH hL h t OJ PJ QJ nHtH #h8 h t 5OJ PJ QJ nHtHh8 h t 56OJ QJ h8 h t 5OJ QJ / I Y Z L
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