Publication Name	World Journal of Gastrointestinal Surgery
Manuscript ID	103494
Country	China

Received	2024-11-21 06:43
Peer-Review Started	2024-11-21 06:43
To Make the First Decision
Return for Revision	2025-01-09 14:59
Revised	2025-01-21 15:27
Second Decision	2025-02-11 02:36
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-02-11 08:15
Articles in Press	2025-02-11 08:15
Publication Fee Transferred	2025-01-26 05:33
Edit the Manuscript by Language Editor	2025-02-13 07:32
Typeset the Manuscript	2025-02-20 01:27
Publish the Manuscript Online	2025-03-29 06:54

ISSN	1948-9366 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Cell & Tissue Engineering
Manuscript Type	Basic Study
Article Title	Single-nucleus RNA sequencing and spatial transcriptomics reveal the mechanism by which Xiaozhiling injection treats internal hemorrhoids
Manuscript Source	Unsolicited Manuscript
All Author List	Min-Hui Ke, Shu-Yan Huang, Wei-Gan Lin, Zhen-Guo Xu, Xia-Xia Zheng, Xian-Bao Liu, You-Min Cheng and Zuan-Fang Li
ORCID	Author(s) ORCID Number Min-Hui Ke http://orcid.org/0000-0003-0930-8901
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation Project No. 81774118 Foreign Cooperation Project of Science and Technology Department of Fujian Province No. 2023I0021 Medical Innovation Project of Fujian Province No. 2024CXB013
Corresponding Author	Min-Hui Ke, Department of Proctology, The Second People’s Hospital Affiliated with Fujian University of Traditional Chinese Medicine, No. 282 Wusi Road, Gulou District, Fuzhou 350003, Fujian Province, China. 48330132@qq.com
Key Words	Internal hemorrhoids; Xiaozhiling injection; Fibroblast; Marker gene; Single-cell sequencing; Spatial transcriptome sequencing
Core Tip	Single-cell sequencing revealed differences in the expression and transcription levels of genes collagen 3 alpha 1, decorin, and actin alpha 2 in fibroblasts between control groups and treated groups. The activation of fibroblasts and the expression of key genes in the sphingosine kinase 1-sphingosine-1-phosphate pathway were observed at the injection site, suggesting that the sphingosine kinase 1-sphingosine-1-phosphate pathway could be activated to promote fibrosis after the intervention of Xiaozhling.
Publish Date	2025-03-29 06:54
Citation	<p>Ke MH, Huang SY, Lin WG, Xu ZG, Zheng XX, Liu XB, Cheng YM, Li ZF. Single-nucleus RNA sequencing and spatial transcriptomics reveal the mechanism by which Xiaozhiling injection treats internal hemorrhoids. <i>World J Gastrointest Surg</i> 2025; 17(4): 103494</p>
URL	https://www.wjgnet.com/1948-9366/full/v17/i4/103494.htm
DOI	https://dx.doi.org/10.4240/wjgs.v17.i4.103494

Full Article (PDF)	WJGS-17-103494-with-cover.pdf
The ARRIVE Guidelines	103494-The-ARRIVE-Guidelines.pdf
Manuscript File	103494_Auto_Edited_012340.docx
Answering Reviewers	103494-answering-reviewers.pdf
Audio Core Tip	103494-audio.m4a
Biostatistics Review Certificate	103494-biostatistics-statement.pdf
Conflict-of-Interest Disclosure Form	103494-conflict-of-interest-statement.pdf
Copyright License Agreement	103494-copyright-assignment.pdf
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Institutional Animal Care and Use Committee Approval Form or Document	103494-institutional-animal-care-and-use-committee-statement.pdf
Non-Native Speakers of English Editing Certificate	103494-non-native-speakers.pdf
Peer-review Report	103494-peer-reviews.pdf
Scientific Misconduct Check	103494-scientific-misconduct-check.png
Scientific Editor Work List	103494-scientific-editor-work-list.pdf
CrossCheck Report	103494-crosscheck-report.pdf