| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Nidaa A Ababneh, Enas Alwohoush, Razan AlDiqs, Mohammad A Ismail, Ban Al-Kurdi, Raghda Barham, Renata Al-Atoom, Fairouz Nairat, Sabal Al Hadidi, Suha Whaibi, Mohammad H Gharandouq, Suzan Zalloum, Sofian Al Shboul, Talal Al-Qaisi, Areej Abuhammad, Tareq Saleh and Abdalla Awidi |
| ORCID |
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| Funding Agency and Grant Number |
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| Corresponding Author |
Nidaa A Ababneh, Associate Professor, Cell Therapy Center, University of Jordan, Queen Rania Street, Amman 11942, Jordan. n.ababneh@ju.edu.jo |
| Key Words |
Induced pluripotent stem cells; Induced mesenchymal stem cells; Regenerative medicine; Differentiation protocols; Tissue engineering |
| Core Tip |
Induced pluripotent stem cell-derived mesenchymal stem cells (MSCs) meet standard MSC criteria, yet their differentiation, proliferation, and functional properties vary considerably depending on the generation protocol. We compared five differentiation strategies, identifying distinct strengths and limitations in osteogenesis, adipogenesis, mitochondrial function, oxidative stress, senescence, and migration. Our findings highlight the need to optimize and standardize differentiation methods to produce reproducible, high-quality induced pluripotent stem cell-derived MSCs for research and therapeutic applications. |
| Publish Date |
2025-12-25 03:52 |
| Citation |
Ababneh NA, Alwohoush E, AlDiqs R, Ismail MA, Al-Kurdi B, Barham R, Al-Atoom R, Nairat F, Al Hadidi S, Whaibi S, Gharandouq MH, Zalloum S, Al Shboul S, Al-Qaisi T, Abuhammad A, Saleh T, Awidi A. Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells. World J Stem Cells 2025; 17(12): 110564 |
| URL |
https://www.wjgnet.com/1948-0210/full/v17/i12/110564.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v17.i12.110564 |
