Publication Name	World Journal of Gastrointestinal Oncology
Manuscript ID	110997
Country	China

Received	2025-07-11 09:46
Peer-Review Started	2025-07-11 09:46
To Make the First Decision
Return for Revision	2025-08-01 08:53
Revised	2025-08-09 07:27
Second Decision	2025-09-10 02:46
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-09-10 07:31
Articles in Press	2025-09-10 07:31
Publication Fee Transferred	2025-08-13 04:27
Edit the Manuscript by Language Editor
Typeset the Manuscript	2025-10-08 09:33
Publish the Manuscript Online	2025-10-14 07:42

ISSN	1948-5204 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
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Category	Gastroenterology & Hepatology
Manuscript Type	Retrospective Study
Article Title	Effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues
Manuscript Source	Unsolicited Manuscript
All Author List	Ting-Ting Wu, Xiang Zhou, Qi Huang and Qi Yang
ORCID	Author(s) ORCID Number Ting-Ting Wu http://orcid.org/0009-0000-2684-834X Xiang Zhou http://orcid.org/0000-0002-4646-2046 Qi Huang http://orcid.org/0009-0003-6631-4926 Qi Yang http://orcid.org/0009-0006-7047-6073
Funding Agency and Grant Number	Funding Agency Grant Number Liyang People's Hospital Technology Project 2021YJKT0013
Corresponding Author	Ting-Ting Wu, Deputy Director, Clinical Laboratory, Liyang People's Hospital, No. 70 Jianshe West Road, Licheng Town, Liyang City, Changzhou 213300, Jiangsu Province, China. wtt15335009416@163.com
Key Words	16S rRNA gene; Multi-region sequencing; Gastric cancer; Species resolution; Microbiome; Alpha diversity
Core Tip	This study demonstrated that multi-region 16S rRNA gene sequencing provides significantly improved species resolution and detection rates compared with traditional single-region method. In both positive controls and gastric cancer tissue samples, it revealed richer and more diverse microbial communities. These findings suggest that compared to single-region sequencing, multi-region sequencing is a more accurate and effective approach for profiling the microbiome in gastric cancer research.
Publish Date	2025-10-14 07:42
Citation	<p>Wu TT, Zhou X, Huang Q, Yang Q. Effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues. <i>World J Gastrointest Oncol</i> 2025; 17(10): 110997</p>
URL	https://www.wjgnet.com/1948-5204/full/v17/i10/110997.htm
DOI	https://dx.doi.org/10.4251/wjgo.v17.i10.110997

Full Article (PDF)	WJGO-17-110997-with-cover.pdf
Manuscript File	110997_Auto_Edited_011751.docx
Answering Reviewers	110997-answering-reviewers.pdf
Audio Core Tip	110997-audio.mp3
Biostatistics Review Certificate	110997-biostatistics-statement.pdf
Conflict-of-Interest Disclosure Form	110997-conflict-of-interest-statement.pdf
Copyright License Agreement	110997-copyright-assignment.pdf
Signed Informed Consent Form(s) or Document(s)	110997-informed-consent-statement.pdf
Institutional Review Board Approval Form or Document	110997-institutional-review-board-statement.pdf
Non-Native Speakers of English Editing Certificate	110997-non-native-speakers.pdf
Peer-review Report	110997-peer-reviews.pdf
Scientific Misconduct Check	110997-scientific-misconduct-check.png
Scientific Editor Work List	110997-scientific-editor-work-list.pdf
CrossCheck Report	110997-crosscheck-report.pdf