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Articles Published Processes
9/3/2026 2:49:01 AM | Browse: 2 | Download: 0
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Received |
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2026-03-23 07:33 |
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Peer-Review Started |
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2026-03-23 07:33 |
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First Decision by Editorial Office Director |
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2026-04-28 10:50 |
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Return for Revision |
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2026-04-28 10:50 |
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Revised |
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2026-05-13 12:31 |
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Publication Fee Transferred |
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Second Decision by Editor |
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2026-06-23 02:17 |
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Second Decision by Editor-in-Chief |
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Final Decision by Editorial Office Director |
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2026-06-23 07:03 |
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Articles in Press |
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2026-06-23 07:03 |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2026-09-01 08:14 |
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Publish the Manuscript Online |
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2026-09-03 02:49 |
| ISSN |
1949-8454 (online) |
| Open Access |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc. |
| Copyright |
©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Transplantation |
| Manuscript Type |
Basic Study |
| Article Title |
Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Rhys R Pook, Abbie Hearne, Rachel A Botting, Samuel J Tingle, Minna Honkanen-Scott, James A M Shaw, Simi Ali and William E Scott III |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Institute for Health and Care Research Blood and Transplant Research Unit in Organ Donation and Transplantation |
No. NIHR203332 |
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| Corresponding Author |
William E Scott III, Associate Professor, PhD, Department of Regenerative Medicine, Stem Cells, and Transplantation, Translational and Clinical Research Institute, Newcastle University, E1.08 Biomedicine West Wing, The International Centre for Life, Times Square, Scotswood Road, Newcastle upon Tyne NE2 4HH, England, United Kingdom. bill.scott3@newcastle.ac.uk |
| Key Words |
Pancreas; RNA; RNA integrity numbers value; RNA isolation; Human biopsy; Organ transplantation; Molecular biology |
| Core Tip |
Obtaining high-quality RNA from human pancreatic tissue remains a persistent challenge in transplant research due to its high endogenous RNase activity and unavoidable pre-analytical ischemia. Using 144 biopsies from four deceased donor pancreata, we systematically evaluated tissue preservation strategies. Our findings demonstrate that both tissue submersion and direct injection of RNAlater yield significantly superior RNA integrity compared with conventional methods. This study provides specific human evidence for a simple and scalable strategy to ensure reliable biobanking and downstream molecular analysis of human pancreatic tissue. |
| Publish Date |
2026-09-03 02:49 |
| Citation |
Pook RR, Hearne A, Botting RA, Tingle SJ, Honkanen-Scott M, Shaw JAM, Ali S, Scott III WE. Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods. World J Biol Chem 2026; 17(3): 121018
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| URL |
https://www.wjgnet.com/1949-8454/full/v17/i3/121018.htm |
| DOI |
https://doi.org/10.4331/wjbc.121018 |
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