Publication Name	World Journal of Biological Chemistry
Manuscript ID	12213
Country	Italy

Received	2014-06-28 14:17
Peer-Review Started	2014-06-28 19:13
To Make the First Decision	2014-07-18 12:34
Return for Revision	2014-07-18 16:55
Revised	2014-08-01 15:40
Second Decision	2014-09-10 13:33
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2014-09-10 13:57
Articles in Press	2014-09-10 13:57
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-11-20 09:13
Publish the Manuscript Online	2014-12-02 12:16

ISSN	1949-8454 (online)
Open Access
Copyright
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Category	Cell Biology
Manuscript Type	Autobiography
Article Title	Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells
Manuscript Source	Invited Manuscript
All Author List	Sara Castiglioni, Clelia Caspani, Alessandra Cazzaniga and Jeanette AM Maier
Funding Agency and Grant Number
Corresponding Author	Jeanette AM Maier, MD, Dipartimento di Scienze Biomediche e Cliniche Luigi Sacco, Università di Milano, Via GB Grassi, 74, Milano 20157, Italy. jeanette.maier@unimi.it
Key Words	Endothelial cells; Nanoparticles; Genotoxicity; Cytotoxicity; Angiogenesis
Core Tip	We studied the sensitivity to silver nanoparticles of microvascular endothelial cells, which are responsible for tissue homeostasis and fundamental in angiogenesis. Silver nanoparticles are cytotoxic and lead to membrane leakage. Cytotoxicity is not prevented by the antioxidants Trolox, N-acetyl-L-cysteine or apocynin. Silver nanoparticles also induce DNA damage as demonstrated by comet assay. When exposed to sublethal concentrations of silver nanoparticles for long times, the cells remain viable but are growth retarded. Interestingly, removal of silver nanoparticle rescue cell growth, suggesting that no permanent modifications occur. Silver nanoparticles are cytotoxic and genotoxic also for endothelial progenitors, which contribute to angiogenesis.
Publish Date	2014-12-02 12:16
Citation	Castiglioni S, Caspani C, Cazzaniga A, Maier JAM. Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells. World J Biol Chem 2014; 5(4): 457-464
URL	http://www.wjgnet.com/1949-8454/full/v5/i4/457.htm
DOI	http://dx.doi.org/10.4331/wjbc.v5.i4.457

Full Article (PDF)	WJBC-5-457.pdf
Full Article (Word)	WJBC-5-457.doc
Manuscript File	12213-Review.doc
Answering Reviewers	12213-Answering reviewers.pdf
Copyright License Agreement	12213-Copyright assignment.pdf
Peer-review Report	12213-Peer review(s).pdf
Scientific Misconduct Check	12213-CrossCheck.jpg
Scientific Editor Work List	12213-Scientific editor work list.pdf