| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc. |
| Copyright |
©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
|
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Surgery |
| Manuscript Type |
Basic Study |
| Article Title |
Establishment of a Sprague-Dawley rat model of hepatolithiasis based on common bile duct catheterization
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Jia-Lin Shi, Tauqeer Muhammad, Ting-Ting He, Zhu-Ying Lin, Tian-Gui Zhang, Shi-Yue Liu, Xiao-Lei Yang, Yao Yang, Lei Hu, Pan Ran, Muhammad Numan and Jiang Li |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| “Xingdian Talents” Support Project of Yunnan Province |
|
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| Corresponding Author |
Jiang Li, Chief Physician, Full Professor, Head, PhD, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Wu Hua District, Kunming 650032, Yunnan Province, China. lijiang1020@kmmu.edu.cn |
| Key Words |
Hepatolithiasis; Sprague-Dawley rat; Lipopolysaccharide; Common bile duct catheterization; Fourier-transform infrared spectroscopy; Lithiasis-associated cholangitis and fibrosis |
| Core Tip |
This study, based on the placement of a bile duct catheter, successfully established a bile duct stone model in Sprague-Dawley rats by administering lipopolysaccharide (1 mg/kg) intra-biliary injections on days 4, 9, 14, and 19 after surgery. The model was validated through multiple methods, including microscopic observation (bile smear), the formation of bilirubin-calcium crystals (Fourier transform infrared spectroscopy), and histopathology (Scheuer score). These findings confirmed that the model consistently produces bilirubin-calcium stones and reproduces typical pathological changes such as chronic biliary inflammation and biliary fibrosis. Additionally, this model allows for repeated intra-biliary dosing and continuous bile sampling, making it a reliable experimental platform for studying the pathogenesis of intrahepatic bile duct stones and evaluating novel intervention strategies. |
| Publish Date |
2026-11-03 04:47 |
| Citation |
Shi JL, Muhammad T, He TT, Lin ZY, Zhang TG, Liu SY, Yang XL, Yang Y, Hu L, Ran P, Numan M, Li J. Establishment of a Sprague-Dawley rat model of hepatolithiasis based on common bile duct catheterization. World J Gastroenterol 2026; 32(48): 122778
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| URL |
https://www.wjgnet.com/1007-9327/full/v32/i48/122778.htm |
| DOI |
https://doi.org/10.3748/wjg.122778 |
