| Category |
Cell & Tissue Engineering |
| Manuscript Type |
Basic Study |
| Article Title |
Density gradient centrifugation vs adherence for rat intestinal mesenchymal stem cell isolation
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Su-Yu Lai, Yun Jiang, Run Yuan, Zhuo Wang, Yi-Chang Wang, Zi-Ting Zhao, Chao-Ying Cai, Meng-Yi Ruan, Yi-Xian Zeng, Bing Ren and Xiao-Feng Wang |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Central High-Level Traditional Chinese Medicine Hospital Clinical Research and Achievement Transformation Capacity Improvement Project |
HLCMHPP2023126 |
| Major Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences |
CI2021A02109 |
|
| Corresponding Author |
Xiao-Feng Wang, Full Professor, MD, Department of Proctology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, No. 5 North Line Pavilion, Xicheng District, Beijing 100053, China. wangxiaofeng74@hotmail.com |
| Key Words |
Intestinal mesenchymal stem cells; Percoll gradient isolation; Cell isolation; Rat; Purity; Flow cytometry; Osteogenic differentiation; Adipogenic differentiation; Chondrogenic differentiation |
| Core Tip |
Intestinal mesenchymal stem cells (MSCs) hold therapeutic potential for intestinal diseases, but standardized isolation protocols for rat intestinal MSCs are lacking. This study systematically compares conventional adherence culture and Percoll density gradient centrifugation for isolating rat intestinal MSCs. Density gradient centrifugation yields cells with higher purity, enhanced proliferation, and superior osteogenic/adipogenic differentiation potential. This simple, cost-effective method addresses the limitations of conventional adherence culture and provides a standardized cell source for intestinal regenerative medicine research. |
| Publish Date |
2026-09-24 06:23 |
| Citation |
Lai SY, Jiang Y, Yuan R, Wang Z, Wang YC, Zhao ZT, Cai CY, Ruan MY, Zeng YX, Ren B, Wang XF. Density gradient centrifugation vs adherence for rat intestinal mesenchymal stem cell isolation. World J Stem Cells 2026; 18(9): 124197
|
| URL |
https://www.wjgnet.com/1948-0210/full/v18/i9/124197.htm |
| DOI |
https://doi.org/10.4252/wjsc.124197 |
