Publication Name	World Journal of Gastroenterology
Manuscript ID	12685
Country	Germany

Received	2014-07-22 08:44
Peer-Review Started	2014-07-22 10:08
To Make the First Decision	2014-08-27 19:17
Return for Revision	2014-09-03 16:04
Revised
Second Decision	2014-11-11 10:08
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-11-11 10:27
Articles in Press	2014-11-11 11:00
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-03-18 17:19
Publish the Manuscript Online	2015-03-30 14:23

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Methodology
Manuscript Type	Basic Study
Article Title	Degradation of intestinal mRNA: A matter of treatment
Manuscript Source	Unsolicited Manuscript
All Author List	Sabine Heumüller-Klug, Carsten Sticht, Karin Kaiser, Elvira Wink, Cornelia Hagl, Lucas Wessel and Karl-Herbert Schäfer
Funding Agency and Grant Number
Corresponding Author	Karl-Herbert Schäfer, MD, Professor, Head of Enteric Nervous System Group, University of Applied Sciences Kaiserslautern, 66482 Zweibrücken, Germany. karl-herbert-schaefer@hs-kl.de
Key Words	Intestinal RNA; Degradation; Storing conditions; Flushing; Cooling
Core Tip	The quality of RNA is crucial for an appropriate RNA analysis. Especially when working with human material, precious samples will often be used for different purposes and can therefore not be frozen immediately. Gut tissue is especially fragile and RNA degrades rapidly if not treated adequately. Under these aspects RNA degradation of different gut sections and gut wall layers regarding their treatment was investigated in this study. Storage, rinsing and preparation conditions are essential for RNA stability. Sufficient and permanent cooling as well as the removal of bacterial contamination leads to a reduced degradation in muscle tissue of the gut.
Publish Date	2015-03-30 14:23
Citation	Heumüller-Klug S, Sticht C, Kaiser K, Wink E, Hagl C, Wessel L, Schäfer KH. Degradation of intestinal mRNA: A matter of treatment. World J Gastroenterol 2015; 21(12): 3499-3508
URL	http://www.wjgnet.com/1007-9327/full/v21/i12/3499.htm
DOI	http://dx.doi.org/10.3748/wjg.v21.i12.3499

Full Article (PDF)	WJG-21-3499.pdf
Full Article (Word)	WJG-21-3499.doc
Manuscript File	12685-Review.doc
Answering Reviewers	12685-Answering reviewers.pdf
Copyright License Agreement	12685-Copyright assignment.pdf
Peer-review Report	12685-Peer review(s).pdf
Scientific Misconduct Check	12685-CrossCheck.jpg
Scientific Editor Work List	12685-Scientific editor work list.pdf