| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Research & Experimental Medicine |
| Manuscript Type |
Basic Study |
| Article Title |
Primary analysis and screening of microRNAs in gastric cancer side population cells
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Hai-Hong Zhang, Guo-Li Gu, Xiang-Yang Zhang, Feng-Zhi Li, Li Ding, Qin Fan, Ran Wu, Wei Shi, Xin-Yan Wang, Lin Chen, Xue-Ming Wei and Xiao-Ying Yuan |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
81000706/H1108 |
| National Key Technology Research and Development Program |
2012BA141B01 |
| Air Force General Hospital of Chinese PLA Innovation Fund |
KZ2013035 |
|
| Corresponding Author |
Xue-Ming Wei, Professor, Department of General Surgery, Air Force General Hospital of Chinese PLA, No. 30 Fucheng Road, Haidian District, Beijing 100142, China. 787939904@qq.com |
| Key Words |
MircoRNA; Stem cells; Stomach neoplasm; Benzimidazoles (Hoe 33342); Side population cells; ATP-binding cassette transporters |
| Core Tip |
MicroRNAs (miRNAs) act as one mechanism by which coding genes are regulated, and they are involved in many biological and pathological processes including carcinogenesis. However, identifying specific miRNAs is difficult, because many miRNAs possess more than one target and many target genes are regulated by more than one miRNA. We used fluorescence-activated cell sorting and Hoechst 33342 labeling to obtain side population (SP) cells from the human gastric carcinoma cell line MKN-45. The miRNA expression profiles of SP and major population cells were examined by miRNA gene chip analysis, and key miRNAs were identified according to aberrant expression and their possible targets as predicted by bioinformatics. |
| Publish Date |
2015-03-30 14:23 |
| Citation |
Zhang HH, Gu GL, Zhang XY, Li FZ, Ding L, Fan Q, Wu R, Shi W, Wang XY, Chen L, Wei XM, Yuan XY. Primary analysis and screening of microRNAs in gastric cancer side population cells. World J Gastroenterol 2015; 21(12): 3519-3526 |
| URL |
http://www.wjgnet.com/1007-9327/full/v21/i12/3519.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v21.i12.3519 |
