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Articles Published Processes
12/2/2015 11:48:00 AM | Browse: 886 | Download: 1845
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Received |
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2015-02-22 03:10 |
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Peer-Review Started |
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2015-02-22 17:37 |
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To Make the First Decision |
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2015-04-27 15:31 |
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Return for Revision |
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2015-04-30 15:16 |
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Revised |
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2015-05-21 05:42 |
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Second Decision |
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2015-06-12 16:32 |
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Accepted by Journal Editor-in-Chief |
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2015-06-13 15:13 |
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Accepted by Executive Editor-in-Chief |
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2015-06-19 17:54 |
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Articles in Press |
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2015-06-19 17:54 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2015-08-15 21:54 |
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Publish the Manuscript Online |
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2015-08-27 10:51 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Simplified three-dimensional culture system for long-term expansion of embryonic stem cells
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Christina McKee, Mick Perez-Cruet, Ferman Chavez and G Rasul Chaudhry |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Oakland University and Oakland University-William Beaumont Institute for Stem Cell and Regenerative Medicine |
OU-WB ISCRM) |
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| Corresponding Author |
Dr. G Rasul Chaudhry, Department of Biological Sciences, Oakland University, 2200 North Squirrel Road, Rochester, MI 48309,
United States. chaudhry@oakland.edu
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| Key Words |
Three-dimensional culture; Pluripotency; Embryonic stem cells; Self-assembling scaffold; Hydrogel |
| Core Tip |
The pluripotent nature of embryonic stem cells (ESCs) makes them an ideal source for cell-based therapeutics and regenerative medicine. Efficient and reproducible expansion of ESCs ex vivo is critical for high quality cells for translational applications. However, propagation of ESCs is technically challenging, and often leads to differentiation due to inefficient two-dimensional culture techniques in vitro. To mimic the three-dimen-sional micro-environment in vivo, self-assembling scaffolds made from thiol-functionalized dextran and polyethylene glycol tetra-acrylate were designed to encapsulate and propagate mouse ESCs. This culture system is simple, robust, efficient and reproducible, permitting long-term maintenance of ESCs without routine passaging and manipulation.
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| Publish Date |
2015-08-27 10:51 |
| Citation |
McKee C, Perez-Cruet M, Chavez F, Chaudhry GR. Simplified three-dimensional culture system for long-term expansion of embryonic stem cells. World J Stem Cells 2015; 7(7): 1064-1077 |
| URL |
http://www.wjgnet.com/1948-0210/full/v7/i7/1064.htm |
| DOI |
http://dx.doi.org/10.4252/wjsc.v7.i7.1064 |
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