Publication Name	World Journal of Gastrointestinal Pathophysiology
Manuscript ID	17306
Country	China

Received	2015-03-04 08:42
Peer-Review Started	2015-03-26 09:16
To Make the First Decision	2015-04-13 15:44
Return for Revision	2015-04-20 16:48
Revised	2015-04-29 23:15
Second Decision	2015-08-21 13:10
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2015-09-07 10:38
Articles in Press	2015-09-07 10:38
Publication Fee Transferred
Edit the Manuscript by Language Editor	2015-12-29 14:36
Typeset the Manuscript	2016-01-25 14:51
Publish the Manuscript Online	2016-01-31 16:11

ISSN	2150-5330 (online)
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Category	Neurosciences
Manuscript Type	Basic Study
Article Title	Neurophysiological mechanisms of bradykinin-evoked mucosal chloride secretion in guinea pig small intestine
Manuscript Source	Unsolicited Manuscript
All Author List	Mei-Hua Qu, Wan-Sheng Ji, Ting-Kun Zhao, Chun-Yan Fang, Shu-Mei Mao and Zhi-Qin Gao
Funding Agency and Grant Number	Funding Agency Grant Number Shandong Provincial Natural Science Foundation, China ZR2009CL047 Shandong Provincial Natural Science Foundation, China ZR2011HM043 Scientific Research Foundation for Returned Scholars Shandong Province Higher Educational Science and Technology Program J14LK15 Excellent Teachers International Training Cooperation Program of Shandong Province for Yang XY and Qu MH
Corresponding Author	Mei-Hua Qu, PhD, MD, Key Lab of Applied Pharmacology in Universities of Shandong, Department of Pharmacology, Weifang Medical University, 7166 Baotong West Street, Weifang 261053, Shandong Province, China. qumeihua@hotmail.com
Key Words	Brandykinin; Ussing chamber; Brandykinin receptor; Cyclooxygenase; Prostaglandin E; Chloride secretion
Core Tip	Bradykinin (BK) can stimulate intestinal chloride secretion and the firing of intestinal secretomotor neurons in the small intestine, but the mechanism is not well understood. In this study, muscle-stripped guinea pig ileal preparations were mounted in Ussing flux chambers for the recording of short-circuit current (Isc). BK agonist and BK antagonist were added to check the Isc change. Inhibitors of the signal transductors were pre-incubated with the tissue for 10 min before evoking with BK, and the Isc change was recorded. The change of prostaglandin E2 (PGE2) secretion was detected by ELISA after treatment with BK for 3 h. Results suggest that BK stimulates neurogenic chloride secretion in the guinea pig ileum by activating B2 receptors on secretomotor neurons, activating cyclooxygenase-1, and stimulating PGE2 production. The post-receptor transduction cascade includes activation of PLC, PKC, CaMK, IP3, and MAPK.
Publish Date	2016-01-31 16:11
Citation	Qu MH, Ji WS, Zhao TK, Fang CY, Mao SM, Gao ZQ. Neurophysiological mechanisms of bradykinin-evoked mucosal chloride secretion in guinea pig small intestine. World J Gastrointest Pathophysiol 2016; 7(1): 150-159
URL	http://www.wjgnet.com/2150-5330/full/v7/i1/150.htm
DOI	http://dx.doi.org/10.4291/wjgp.v7.i1.150

Full Article (PDF)	WJGP-7-150.pdf
Full Article (Word)	WJGP-7-150.doc
Manuscript File	17306-Review.doc
Answering Reviewers	17306-Answering reviewers.pdf
Biostatistics Review Certificate	17306-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	17306-Conflict-of-interest statement.pdf
Copyright License Agreement	17306-Copyright assigment.pdf
Institutional Review Board Approval Form or Document	17306-Institutional animal care and use committee statement.pdf
Non-Native Speakers of English Editing Certificate	17306-Language certificate.pdf
Peer-review Report	17306-Peer-review(s).pdf
Scientific Misconduct Check	17306-Scientific misconduct check.pdf
Scientific Editor Work List	17306-Scientific editor work list.pdf