Publication Name	World Journal of Methodology
Manuscript ID	17502
Country	Japan

Received	2015-03-10 18:02
Peer-Review Started	2015-03-18 19:42
To Make the First Decision	2015-05-13 19:52
Return for Revision	2015-05-18 19:18
Revised	2015-05-22 21:32
Second Decision	2015-08-20 16:19
Accepted by Journal Editor-in-Chief	2015-08-20 17:48
Accepted by Company Editor-in-Chief	2015-08-31 15:44
Articles in Press	2015-08-31 15:44
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-09-01 15:22
Publish the Manuscript Online	2015-09-16 14:40

ISSN	2222-0682 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Gastroenterology & Hepatology
Manuscript Type	Editorial
Article Title	Hepatocyte selection medium eliminating induced pluripotent stem cells among primary human hepatocytes
Manuscript Source	Invited Manuscript
All Author List	Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto and Naoki Ishige
Funding Agency and Grant Number
Corresponding Author	Minoru Tomizawa, MD, PhD, Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan. nihminor-cib@umin.ac.jp
Key Words	Ornithine transcarbamylase; Galactokinase; Arginine; Galactose; Urea cycle
Core Tip	Human induced pluripotent stem (hiPS) cells have the potential to differentiate into mature hepatocytes. If undifferentiated hiPS cells persist among transplanted hepatocytes, hiPS cells may potentially develop into cancer. Glucose and arginine are essential for the survival of most cells; however, mature hepatocytes survive in the media because they can produce glucose and arginine using galactokinase and ornithine transcarbamylase, respectively. Therefore, we created a hepatocyte selection medium (HSM) that lacks glucose and arginine but is supplemented with galactose and ornithine. After establishment of co-culture in HSM, human primary hepatocytes survive while hiPS cells die within three days.
Publish Date	2015-09-16 14:40
Citation	Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N. Hepatocyte selection medium eliminating induced pluripotent stem cells among primary human hepatocytes. World J Methodol 2015; 5(3): 108-114
URL	http://www.wjgnet.com/2222-0682/full/v5/i3/108.htm
DOI	http://dx.doi.org/10.5662/wjm.v5.i3.108

Full Article (PDF)	WJM-5-108.pdf
Full Article (Word)	WJM-5-108.doc
Manuscript File	17502-Review.doc
Answering Reviewers	17502-Answering reviewers.pdf
Audio Core Tip	17502-Audio core tip.MP3
Conflict-of-Interest Disclosure Form	17502-Conflict-of-interest statement.pdf
Copyright License Agreement	17502-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	Editing_Certificate_20150310135118.pdf
Peer-review Report	17502-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	17502-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	17502-Scientific misconduct check.pdf
Scientific Editor Work List	17502-Scientific editor work list.pdf