Publication Name	World Journal of Stem Cells
Manuscript ID	18188
Country	China

Received	2015-04-10 15:53
Peer-Review Started	2015-04-12 00:06
To Make the First Decision	2015-05-14 16:45
Return for Revision	2015-05-19 09:21
Revised	2015-05-25 15:52
Second Decision	2015-07-29 18:29
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2015-08-07 16:57
Articles in Press	2015-08-07 16:58
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-09-15 19:50
Publish the Manuscript Online	2015-09-25 17:30

ISSN	1948-0210 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Hematology
Manuscript Type	Basic Study
Article Title	Easily-handled method to isolate mesenchymal stem cells from coagulated human bone marrow samples
Manuscript Source	Invited Manuscript
All Author List	Heng-Xiang Wang, Zhi-Yong Li, Zhi-Kun Guo and Zi-Kuan Guo
Funding Agency and Grant Number	Funding Agency Grant Number Natural Science Foundation of China 30971068 Natural Science Foundation of China 81170522
Corresponding Author	Zi-Kuan Guo, MD, PhD, Department of Experimental Hematology, Beijing Radiation Medicine, 27# Taiping Road, Beijing 100850, China. guozk@bmi.ac.cn
Key Words	Coagulated marrow sample; Mesenchymal stem cells; Isolation; Urokinase
Core Tip	It is usually difficult to isolate mesenchymal stem cells (MSCs) from bone marrow samples that have been coagulated due to inappropriate marrow aspirations or in the setting of pathologically hypercoagulative state. In this study, the authors found that urokinase pretreatment could dissolve stem cells from the clots. The MSCs were fibroblat-like in morphology and homogenously positive for CD44, CD73 and CD90, and negative for CD31 and CD45. Also, they could be induced to differentiate into osteoblasts and adipocytes in vitro. Urokinase pretreatment is an optimal strategy to isolate MSCs from poorly aspirated and clotted human bone marrow samples.
Publish Date	2015-09-25 17:30
Citation	Wang HX, Li ZY, Guo ZK, Guo ZK. Easily-handled method to isolate mesenchymal stem cells from coagulated human bone marrow samples. World J Stem Cells 2015; 7(8): 1137-1144
URL	http://www.wjgnet.com/1948-0210/full/v7/i8/1137.htm
DOI	http://dx.doi.org/10.4252/wjsc.v7.i8.1137

Full Article (PDF)	WJSC-7-1137.pdf
Full Article (Word)	WJSC-7-1137.doc
Manuscript File	18188-Review.doc
Answering Reviewers	18188-Answering reviewers.pdf
Audio Core Tip	18188-Audio core tip.mp3
Biostatistics Review Certificate	18188-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	18188-Conflict-of-interest statement.pdf
Copyright License Agreement	18188-Copyright assignment.pdf
Institutional Review Board Approval Form or Document	18188-Institutional review board statement.pdf
Peer-review Report	18188-Peer-review(s).pdf
Scientific Misconduct Check	18188-Scientific misconduct check.pdf
Scientific Editor Work List	18188-Scientific editor work list.pdf