Publication Name	World Journal of Virology
Manuscript ID	20174
Country	Canada

Received	2015-05-29 17:14
Peer-Review Started	2015-06-02 08:59
First Decision by Editorial Office Director
Return for Revision	2015-06-25 17:25
Revised	2015-08-01 06:36
Publication Fee Transferred
Second Decision by Editor	2015-09-21 17:45
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2015-09-30 18:43
Articles in Press	2015-09-30 18:43
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-10-21 18:51
Publish the Manuscript Online	2015-11-12 17:09

ISSN	2220-3249 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Virology
Manuscript Type	Basic Study
Article Title	Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis
Manuscript Source	Invited Manuscript
All Author List	Yupin Tong, Bonita E Lee and Xiaoli L Pang
Funding Agency and Grant Number
Corresponding Author	Xiaoli L Pang, PhD, Provincial Laboratory for Public Health, University of Alberta Hospital, WMC 2B4.58, 8440 112 Street, Edmonton, Alberta T6G 2J2, Canada. xiao-li.pang@albertahealthservices.ca
Key Words	Rotavirus A; Melting temperature; Real-time polymerase chain reaction; SYBR green; Genotyping
Core Tip	Genotyping rotavirus is essential for monitoring strain shifts in rotavirus surveillance and vaccine evaluation. Current conventional semi-nested real-time reverse transcription-polymerase chain reaction (RT-PCR), the most commonly used rotavirus genotyping assay is a labor-intensive, complex multi-step procedure and has long turn around-time. The newly developed SYBR Green real time RT-PCR assay is simple, fast and has comparable sensitivity and specificity as conven-tional semi-nested RT-PCR. This new assay was used to genotype clinical samples which tested positive for rotavirus from January 2012 to June 2013 and new emerging G12 strains were identified in Alberta, Canada.
Publish Date	2015-11-12 17:09
Citation	Tong Y, Lee BE, Pang XL. Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis. World J Virol 2015; 4(4): 365-371
URL	http://www.wjgnet.com/2220-3249/full/v4/i4/365.htm
DOI	http://dx.doi.org/10.5501/wjv.v4.i4.365

Full Article (PDF)	WJV-4-365.pdf
Full Article (Word)	WJV-4-365.doc