Publication Name	World Journal of Gastroenterology
Manuscript ID	2198
Country	Brazil

Received	2013-02-05 11:15
Peer-Review Started	2013-02-07 09:23
First Decision by Editorial Office Director
Return for Revision	2013-07-16 10:55
Revised	2013-08-06 05:17
Publication Fee Transferred
Second Decision by Editor	2013-08-20 12:06
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2013-08-20 13:30
Articles in Press
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-10-22 15:28
Publish the Manuscript Online	2013-11-16 18:29

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Genetics & Heredity
Manuscript Type	Autobiography
Article Title	Reference genes for quantitative RT-PCR data in gastric tissues and cell lines
Manuscript Source	Invited Manuscript
All Author List	Fernanda Wisnieski, Danielle Queiroz Calcagno, Mariana Ferreira Leal, Leonardo Caires dos Santos, Carolina de Oliveira Gigek, Elizabeth Suchi Chen, Thaís Brilhante Pontes, Paulo Pimentel Assumpção, Mônica Barauna de Assumpção, Sâmia Demachki, Rommel Rodríguez Burbano and Marília de Arruda Cardoso Smith
Funding Agency and Grant Number	Funding Agency Grant Number Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) to Wisnieski F and Gigek CO Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to Burbano RR and Smith MAC Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) to Wisnieski F, Calcagno DQ, Leal M, Pontes TB and Smith MAC as grants and fellowship awards
Corresponding Author	Fernanda Wisnieski, PhD, Genetics Division, Department of Morphology and Genetic, Federal University of São Paulo, São Paulo 04023900, Brazil. fernandawis@yahoo.com.br
Key Words	Gastric cancer; Reference gene; Normalization; Gene expression; Quantitative real-time polymerase chain reaction
Core Tip	Gene expression studies have revealed much about the molecular basis of gastric cancer. However, the normalization of expression data using reference genes without validation may undermine the results. In the present study, we evaluated the suitability of possible reference genes in gastric tissues and cell lines. To our knowledge, our study is the first to determine and validate reference genes for gastric samples in a Western population. In addition, the inclusion of normal gastric tissues from patients without cancer in determining the best reference genes is original in the literature.
Publish Date	2013-11-16 18:29
Citation	Wisnieski F, Calcagno DQ, Leal MF, Santos LC, Gigek CO, Chen ES, Pontes TB, Assumpção PP, Assumpção MB, Demachki S, Burbano RR, Smith MAC. Reference genes for quantitative RT-PCR data in gastric tissues and cell lines. World J Gastroenterol 2013; 19(41): 7121-7128
URL	http://www.wjgnet.com/1007-9327/full/v19/i41/7121.htm
DOI	http://dx.doi.org/10.3748/wjg.v19.i41.7121

Full Article (PDF)	WJG-19-7121.pdf
Manuscript File	2198-Review.doc
Answering Reviewers	2198-Answering reviewers.pdf
Biostatistics Review Certificate	Biostatistician Review Report.docx
Copyright License Agreement	2198-Copyright assignment.doc
Institutional Review Board Approval Form or Document	Ethics Committee.pdf
Non-Native Speakers of English Editing Certificate	2198-Language certificate.docx
Peer-review Report	2198-Peer reviewer(s).pdf