Publication Name	World Journal of Gastroenterology
Manuscript ID	2331
Country	China

Received	2013-02-17 09:26
Peer-Review Started	2013-02-18 10:09
To Make the First Decision	2013-03-11 14:55
Return for Revision	2013-06-04 09:38
Revised
Second Decision	2013-09-17 09:45
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2013-09-17 11:27
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor	2013-11-19 09:13
Typeset the Manuscript	2013-11-21 16:17
Publish the Manuscript Online	2013-12-11 16:13

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Infectious Diseases
Manuscript Type	Autobiography
Article Title	Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line
Manuscript Source	Invited Manuscript
All Author List	Xiu-Guang Song, Peng-Fei Bian, Shu-Li Yu, Xiu-Hua Zhao, Wei Xu, Xue-Hui Bu, Xia Li and Li-Xian Ma
Funding Agency and Grant Number	Funding Agency Grant Number Jinan Science and Technology Bureau, Shandong Province, China 200705095-4
Corresponding Author	Li-Xian Ma, MD, Department of Infectious Diseases, Qilu Hospital, Shandong University, No. 107, Wenhua Xilu, Jinan 250012, Shandong Province, China. shandongqilu123@gmail.com
Key Words	SV40 T-antigen; Mouse hepatic cell; Hepatitis B virus 1.3-fold genome plasmids; Immortalized; Liposomes; Transfection
Core Tip	This study established a new immortalized mouse hepatic cell line through the transfection of the pRSV-T plasmid. SV40 T-antigen (SV40LT)-immortalized mouse hepatic cells had the same morphology and biological characteristics as primary mouse hepatic cells. SV40LT-immortalized mouse hepatic cells could be transfected with the pHBV1.3 plasmid, which caused the hepatitis B virus (HBV) genes to replicate in SV40LT-immortalized mouse hepatic cells. The expressions of hepatitis B surface antigen and hepatitis B c antigen, as well as the presence of HBV DNA replication intermediates, were observed in the pHBV1.3-transfected cells. This cell model will contribute to the research of HBV and the evaluation of anti-viral drugs in vivo.
Publish Date	2013-12-11 16:13
Citation	Song XG, Bian PF, Yu SL, Zhao XH, Xu W, Bu XH, Li X, Ma LX. Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line. World J Gastroenterol 2013; 19(44): 8020-8027
URL	http://www.wjgnet.com/1007-9327/full/v19/i44/8020.htm
DOI	http://dx.doi.org/10.3748/wjg.v19.i44.8020

Full Article (PDF)	WJG-19-8020.pdf
Manuscript File	2331-Review.doc
Answering Reviewers	2331-Answering reviewers.pdf
Copyright License Agreement	2331-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	2331-Language certificate.pdf
Peer-review Report	2331-Peer reviews.pdf
Scientific Editor Work List	2331-Scientific editor work list.doc