BPG is committed to discovery and dissemination of knowledge
Articles Published Processes
7/4/2016 2:42:00 PM | Browse: 993 | Download: 1599
Publication Name World Journal of Gastroenterology
Manuscript ID 23868
Country/Territory China
Received
2015-12-31 14:14
Peer-Review Started
2015-12-31 14:49
To Make the First Decision
2016-03-21 09:32
Return for Revision
2016-03-22 10:46
Revised
2016-04-08 14:04
Second Decision
2016-04-13 07:54
Accepted by Journal Editor-in-Chief
2016-04-13 08:33
Accepted by Company Editor-in-Chief
2016-04-20 14:16
Articles in Press
2016-04-20 14:16
Publication Fee Transferred
Edit the Manuscript by Language Editor
2016-06-08 02:17
Typeset the Manuscript
2016-06-17 17:08
Publish the Manuscript Online
2016-07-04 14:42
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Microbiology
Manuscript Type Observational Study
Article Title Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori
Manuscript Source Unsolicited Manuscript
All Author List Xiao-Feng Luo, Jian-Hua Jiao, Wen-Yue Zhang, Han-Ming Pu, Bao-Jin Qu, Bing-Ya Yang, Min Hou and Min-Jun Ji
Funding Agency and Grant Number
Corresponding Author Dr. Min-Jun Ji, Department of Pathogen Biology, Nanjing Medical University, No. 101 Longmian Dadao, Nanjing 211166, Jiangsu Province, China. jiminjun@njmu.edu.cn
Key Words Helicobacter pylori; Nested-allele specific primer-polymerase chain reaction; Rapid urease test; Clarithromycin resistance; Drug sensitivity testing
Core Tip In recent years, antibiotic resistance in Helico-bacter pylori (H. pylori) has become a global problem, especially the resistances towards metronidazole, clarithromycin and amoxicillin. To combat this growing problem, it is important to determine antimicrobial resistance of a patient’s infection before treatment. Normally, the detection of clarithromycin resistance is based mainly on phenotypic methods performed after culturing. However, bacterial culture has many inherent disadvantages. In the present study, the nested-allele specific primer-polymerase chain reaction was established to detect the different mutations at positions 2142, 2143 and 2144 in the 23SrRNA gene of H. pylori, which can be completed within several hours. This method is expected to contribute towards improving the efficacy of H. pylori eradication therapy.
Publish Date 2016-07-04 14:42
Citation Luo XF, Jiao JH, Zhang WY, Pu HM, Qu BJ, Yang BY, Hou M, Ji MJ. Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori. World J Gastroenterol 2016; 22(25): 5822-5830
URL http://www.wjgnet.com/1007-9327/full/v22/i25/5822.htm
DOI http://dx.doi.org/10.3748/wjg.v22.i25.5822
Full Article (PDF) WJG-22-5822.pdf
Full Article (Word) WJG-22-5822.doc
Manuscript File 23868-Review.doc
Answering Reviewers 23868-Answering reviewers.pdf
Audio Core Tip 23868-Audio core tip.m4a
Clinical Trial Registration Statement DeclarationOf_HelsinkiConformation_Statement_20151231115120.zip
Conflict-of-Interest Disclosure Form 23868-Conflict-of-interest statement.pdf
Copyright License Agreement 23868-Copyright assignment.pdf
Signed Informed Consent Form(s) or Document(s) Informed_Consent_Statement_20151231114846.zip
Institutional Review Board Approval Form or Document 23868-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate Editing_Certificate_20151231115428.zip
Supplementary Material 23868-Supplementary material.doc
Peer-review Report 23868-Peer-review(s).pdf
Journal Editor-in-Chief Review Report 23868-Journal editor-in-chief review report.pdf
Scientific Misconduct Check 23868-Scientific misconduct check.pdf
Scientific Editor Work List 23868-Scientific editor work list.pdf