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Articles Published Processes
7/4/2016 2:42:00 PM | Browse: 1108 | Download: 2083
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Received |
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2015-12-31 14:14 |
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Peer-Review Started |
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2015-12-31 14:49 |
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To Make the First Decision |
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2016-03-21 09:32 |
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Return for Revision |
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2016-03-22 10:46 |
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Revised |
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2016-04-08 14:04 |
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Second Decision |
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2016-04-13 07:54 |
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Accepted by Journal Editor-in-Chief |
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2016-04-13 08:33 |
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Accepted by Executive Editor-in-Chief |
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2016-04-20 14:16 |
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Articles in Press |
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2016-04-20 14:16 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2016-06-08 02:17 |
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Typeset the Manuscript |
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2016-06-17 17:08 |
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Publish the Manuscript Online |
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2016-07-04 14:42 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Microbiology |
| Manuscript Type |
Observational Study |
| Article Title |
Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Xiao-Feng Luo, Jian-Hua Jiao, Wen-Yue Zhang, Han-Ming Pu, Bao-Jin Qu, Bing-Ya Yang, Min Hou and Min-Jun Ji |
| Funding Agency and Grant Number |
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| Corresponding Author |
Dr. Min-Jun Ji, Department of Pathogen Biology, Nanjing Medical University, No. 101 Longmian Dadao, Nanjing 211166, Jiangsu Province, China. jiminjun@njmu.edu.cn |
| Key Words |
Helicobacter pylori; Nested-allele specific primer-polymerase chain reaction; Rapid urease test; Clarithromycin resistance; Drug sensitivity testing |
| Core Tip |
In recent years, antibiotic resistance in Helico-bacter pylori (H. pylori) has become a global problem, especially the resistances towards metronidazole, clarithromycin and amoxicillin. To combat this growing problem, it is important to determine antimicrobial resistance of a patient’s infection before treatment. Normally, the detection of clarithromycin resistance is based mainly on phenotypic methods performed after culturing. However, bacterial culture has many inherent disadvantages. In the present study, the nested-allele specific primer-polymerase chain reaction was established to detect the different mutations at positions 2142, 2143 and 2144 in the 23SrRNA gene of H. pylori, which can be completed within several hours. This method is expected to contribute towards improving the efficacy of H. pylori eradication therapy. |
| Publish Date |
2016-07-04 14:42 |
| Citation |
Luo XF, Jiao JH, Zhang WY, Pu HM, Qu BJ, Yang BY, Hou M, Ji MJ. Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori. World J Gastroenterol 2016; 22(25): 5822-5830 |
| URL |
http://www.wjgnet.com/1007-9327/full/v22/i25/5822.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v22.i25.5822 |
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