Publication Name	World Journal of Methodology
Manuscript ID	2542
Country	United States

Received	2013-02-27 09:20
Peer-Review Started	2013-02-27 16:00
To Make the First Decision	2013-03-04 10:55
Return for Revision	2013-03-04 15:08
Revised	2013-03-07 06:02
Second Decision	2013-04-18 16:42
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2013-04-19 06:31
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-05-10 11:37
Publish the Manuscript Online	2013-05-23 15:29

ISSN	2222-0682 (online)
Open Access
Copyright
Article Reprints	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Cell Biology
Manuscript Type	Autobiography
Article Title	Methods for the extraction and RNA profiling of exosomes
Manuscript Source	Invited Manuscript
All Author List	Emily Zeringer, Mu Li, Tim Barta, Jeoffrey Schageman, Ketil Winther Pedersen, Axl Neurauter, Susan Magdaleno, Robert Setterquist and Alexander V Vlassov
Funding Agency and Grant Number
Corresponding Author	Alexander V Vlassov, PhD, Life Technologies, 2130 Woodward street, Austin, TX 78744, United States. sasha.vlassov@lifetech.com
Key Words	Exosomes; Microvesicles; Cell culture media; Serum; RNA; Quantitative reverse transcription-polymerase chain reaction; Sequencing
Core Tip	Exosomes are small vesicles (30-150 nm) perceived to be carriers of the unique effector or signaling macromolecules (miRNA, ncRNA, mRNA and protein) between very specific cells within our body. The spectrum of current scientific interests ranges from studying the functions and pathways of exosomes to utilizing them in diagnostics and therapeutics development. Here we describe a complete exosome workflow solution: fast and efficient isolation of exosomes; extraction of their cargo; characterization of exosomal RNA content using quantitative reverse transcription-polymerase chain reaction and other techniques.
Publish Date	2013-05-23 15:29
Citation	Zeringer E, Li M, Barta T, Schageman J, Pedersen KW, Neu-rauter A, Magdaleno S, Setterquist R, Vlassov AV. Methods for the extraction and RNA profiling of exosomes. World J Methodol 2013; 3(1): 11-18
URL	http://www.wjgnet.com/2222-0682/full/v3/i1/11.htm
DOI	http://dx.doi.org/10.4329/wjm.v3.i1.11

Manuscript File	2542-Review.doc
Answering Reviewers	2542-Answering reviewers.docx
Copyright License Agreement	2542-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	2542-Language cetificate.pdf
Peer-review Report	2542-Peer review.pdf
Scientific Editor Work List	2542-Scientific editor work list.doc