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9/11/2014 8:36:00 AM | Browse: 289 | Download: 421
Publication Name World Journal of Methodology
Manuscript ID 2542
Country/Territory United States
Received
2013-02-27 09:20
Peer-Review Started
2013-02-27 16:00
To Make the First Decision
2013-03-04 10:55
Return for Revision
2013-03-04 15:08
Revised
2013-03-07 06:02
Second Decision
2013-04-18 16:42
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2013-04-19 06:31
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2013-05-10 11:37
Publish the Manuscript Online
2013-05-23 15:29
ISSN 2222-0682 (online)
Open Access
Copyright
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Cell Biology
Manuscript Type Autobiography
Article Title Methods for the extraction and RNA profiling of exosomes
Manuscript Source Invited Manuscript
All Author List Emily Zeringer, Mu Li, Tim Barta, Jeoffrey Schageman, Ketil Winther Pedersen, Axl Neurauter, Susan Magdaleno, Robert Setterquist and Alexander V Vlassov
Funding Agency and Grant Number
Corresponding author Alexander V Vlassov, PhD, Life Technologies, 2130 Woodward street, Austin, TX 78744, United States. sasha.vlassov@lifetech.com
Key Words Exosomes; Microvesicles; Cell culture media; Serum; RNA; Quantitative reverse transcription-polymerase chain reaction; Sequencing
Core Tip Exosomes are small vesicles (30-150 nm) perceived to be carriers of the unique effector or signaling macromolecules (miRNA, ncRNA, mRNA and protein) between very specific cells within our body. The spectrum of current scientific interests ranges from studying the functions and pathways of exosomes to utilizing them in diagnostics and therapeutics development. Here we describe a complete exosome workflow solution: fast and efficient isolation of exosomes; extraction of their cargo; characterization of exosomal RNA content using quantitative reverse transcription-polymerase chain reaction and other techniques.
Publish Date 2013-05-23 15:29
Citation Zeringer E, Li M, Barta T, Schageman J, Pedersen KW, Neu-rauter A, Magdaleno S, Setterquist R, Vlassov AV. Methods for the extraction and RNA profiling of exosomes. World J Methodol 2013; 3(1): 11-18
Url http://www.wjgnet.com/2222-0682/full/v3/i1/11.htm
DOI http://dx.doi.org/10.4329/wjm.v3.i1.11
Manuscript File 2542-Review.doc
Answering Reviewers 2542-Answering reviewers.docx
Copyright License Agreement 2542-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate 2542-Language cetificate.pdf
Peer-review Report 2542-Peer review.pdf
Scientific Editor Work List 2542-Scientific editor work list.doc