Publication Name	World Journal of Clinical Oncology
Manuscript ID	27779
Country	Denmark

Received	2016-06-16 09:27
Peer-Review Started	2016-06-18 08:25
To Make the First Decision	2016-08-04 18:25
Return for Revision	2016-08-16 11:32
Revised	2016-09-12 22:27
Second Decision	2016-11-22 17:44
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2016-12-28 15:28
Articles in Press	2016-12-28 15:28
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2017-01-17 16:50
Publish the Manuscript Online	2017-02-08 15:32

ISSN	2218-4333 (online)
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Category	Oncology
Manuscript Type	Basic Study
Article Title	NDRG2 gene copy number is not altered in colorectal carcinoma
Manuscript Source	Invited Manuscript
All Author List	Anders Lorentzen and Cathy Mitchelmore
Funding Agency and Grant Number	Funding Agency Grant Number Danish Cancer Society DP05117
Corresponding Author	Cathy Mitchelmore, PhD, Associate Professor, Department of Science and Environment, Roskilde University, Universitetsvej 1, Postbox 260, 4000 Roskilde, Denmark. mitch@ruc.dk
Key Words	N-myc downstream-regulated gene 2; Colorectal carcinoma; MYC; Tumor suppressor; Allelic loss; Gene amplification; Copy number
Core Tip	NDRG2 is a putative tumor suppressor gene whose expression is reduced in many cancer forms, including colorectal carcinoma (CRC). We set out there-fore to investigate if down-regulation of NDRG2 expression was due to loss of one or both alleles and/or to other mechanisms. In our paper, we show that allelic loss of NDRG2 is a rare event in CRC. To our knowledge, this is the first study that has specifically investigated gene copy number of NDRG2 in CRC. Furthermore, our results suggest that MYC is amplified in more than 40% of CRC cases. MYC is known to repress transcription of NDRG2. Our results lead us to suggest that it is the transcriptional control of NDRG2 expression, including repression by MYC and epigenetic regulation, that results in decreased NDRG2 mRNA levels in CRC, rather than allelic loss of NDRG2.
Publish Date	2017-02-08 15:32
Citation	Lorentzen A, Mitchelmore C. NDRG2 gene copy number is not altered in colorectal carcinoma. World J Clin Oncol 2017; 8(1): 67-74
URL	http://www.wjgnet.com/2218-4333/full/v8/i1/67.htm
DOI	http://dx.doi.org/10.5306/wjco.v8.i1.67

Full Article (PDF)	WJCO-8-67.pdf
Full Article (Word)	WJCO-8-67.doc
Manuscript File	27779-Review.docx
Answering Reviewers	27779-Answering reviewers.pdf
Audio Core Tip	27779-Audio core tip.m4a
Conflict-of-Interest Disclosure Form	27779-Conflict-of-interest statement.pdf
Copyright License Agreement	27779-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	27779-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	27779-Institutional review board statement.pdf
Supplementary Material	27779-Supplementary material.pdf
Peer-review Report	27779-Peer-review(s).pdf
Scientific Misconduct Check	27779-Scientific misconduct check.pdf
Scientific Editor Work List	27779-Scientific editor work list.pdf