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Articles Published Processes
9/26/2016 8:04:00 AM | Browse: 616 | Download: 1233
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Received |
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2016-07-22 09:25 |
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Peer-Review Started |
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2016-07-22 18:43 |
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To Make the First Decision |
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2016-08-29 09:18 |
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Return for Revision |
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2016-08-29 10:03 |
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Revised |
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2016-09-01 07:16 |
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Second Decision |
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2016-09-07 18:22 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Company Editor-in-Chief |
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2016-09-08 13:22 |
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Articles in Press |
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2016-09-08 13:22 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2016-09-17 06:59 |
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Typeset the Manuscript |
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2016-09-18 18:09 |
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Publish the Manuscript Online |
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2016-09-26 08:04 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Hepatocyte isolation from resected benign tissues: Results of a 5-year experience
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Fan-Ying Meng, Li Liu, Jun Liu, Chun-You Li, Jian-Ping Wang, Feng-Hui Yang, Zhi-Shui Chen and Ping Zhou |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Major Scientific and Technological Project of Shandong Province, China |
201221019 |
| Major Scientific and Technological Project of Shandong Province, China |
2014GSF118178 |
| Cisco Clinical Oncology Research Fund and Bayer Schering Cancer Research Fund |
Y-B2012-011 |
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| Corresponding Author |
Feng-Hui Yang, MD, PhD, Division of Hepato-Biliary-Pancreatic Surgery, Institute of Organ Transplantation, Shandong Provincial Hospital affiliated to Shandong University, No. 324 Jingwuweiqi Road, Jinan 250021, Shandong Province, China. yangfenghui1966@163.com |
| Key Words |
Human hepatocyte; Primary hepatocyte; Cell isolation; Benign liver disease; Hepatocyte isolation |
| Core Tip |
We retrospectively analyzed a 5-year experience of human hepatocyte isolation from surgically-resected normal tissues and established an efficient technique for a special kind of liver samples for large-scale human hepatocyte isolation. Our study represents the largest number of primary human hepatocytes isolated from resected specimens from patients with benign liver disease. We evaluated the effect of donor liver characteristics on cell isolation, and we found that samples of liver hemangioma can provide better results than intrahepatic duct calculi, in terms of cell yield. Furthermore, the size of the tissues can affect the outcome of hepatocyte isolation. |
| Publish Date |
2016-09-26 08:04 |
| Citation |
Meng FY, Liu L, Liu J, Li CY, Wang JP, Yang FH, Chen ZS, Zhou P. Hepatocyte isolation from resected benign tissues: Results of a 5-year experience. World J Gastroenterol 2016; 22(36): 8178-8186 |
| URL |
http://www.wjgnet.com/1007-9327/full/v22/i36/8178.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v22.i36.8178 |
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