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Articles Published Processes
1/12/2017 1:49:00 PM | Browse: 1066 | Download: 1972
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Received |
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2016-08-03 10:46 |
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Peer-Review Started |
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2016-08-03 14:53 |
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First Decision by Editorial Office Director |
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Return for Revision |
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2016-09-23 15:48 |
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Revised |
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2016-10-05 16:36 |
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Publication Fee Transferred |
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Second Decision by Editor |
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2016-10-24 16:45 |
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Second Decision by Editor-in-Chief |
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Final Decision by Editorial Office Director |
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2016-11-02 08:13 |
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Articles in Press |
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2016-11-02 08:13 |
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Edit the Manuscript by Language Editor |
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2016-12-01 05:07 |
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Typeset the Manuscript |
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2016-12-29 17:02 |
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Publish the Manuscript Online |
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2017-01-12 13:49 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Methodology |
| Manuscript Type |
Basic Study |
| Article Title |
Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Hong-Bo Shi, Jin-Li Lou, Hong-Lin Shi, Feng Ren, Yu Chen and Zhong-Ping Duan |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
81300349 |
| National Natural Science Foundation of China |
81270532 |
| Beijing Natural Science Foundation |
7144216 |
| Beijing Nova Program |
Z131107000413016 |
| Project of Science and Technology Activities of preferred overseas personnel of Beijing (2014) |
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| the Project of Cultivation of High Level Medical Technical Personnel in Health System of Beijing |
2014-3-090 |
| the Project of Cultivation of High Level Medical Technical Personnel in Health System of Beijing |
2013-3-071 |
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| Corresponding Author |
Zhong-Ping Duan, MD, PhD, Professor, Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, 8 Xitoutiao, Youwai Street, Fengtai District, Beijing 100069, China. duan2517@163.com |
| Key Words |
Bacterial artificial chromosome; Homologous recombination; Glycoprotein M6a; Reelin |
| Core Tip |
Until now, there have been few specific mouse lines that allowed recombination for tracing hepatic mesothelial cells or hepatic stellate cells. Here, we describe a rapid and reliable strategy for construct preparation using a bacterial artificial chromosome. This study prepared a Gpm6a/ReelinGFPCreERT2 construct for the first time, which is the first step for the preparation of a Gpm6aGFPCreERT2 or ReelinGFPCreERT2 mouse line. |
| Publish Date |
2017-01-12 13:49 |
| Citation |
Shi HB, Lou JL, Shi HL, Ren F, Chen Y, Duan ZP. Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells. World J Gastroenterol 2017; 23(2): 224-231
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| URL |
http://www.wjgnet.com/1007-9327/full/v23/i2/224.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v23.i2.224 |
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