Publication Name	World Journal of Stem Cells
Manuscript ID	2935
Country	Singapore

Received	2013-03-26 14:26
Peer-Review Started	2013-03-28 09:28
To Make the First Decision	2013-04-16 09:14
Return for Revision	2013-04-18 14:21
Revised	2013-05-07 16:03
Second Decision	2013-06-08 14:12
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2013-06-10 04:10
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-06-28 12:01
Publish the Manuscript Online	2013-07-25 08:11

ISSN	1948-0210 (online)
Open Access
Copyright
Article Reprints	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Developmental Biology
Manuscript Type	Autobiography
Article Title	Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells
Manuscript Source	Invited Manuscript
All Author List	Sherwin Ting, Marti Lecina, Yau-Chi Chan, Hung Fat Tse, Shaul Reuveny and Steve KW Oh
Funding Agency and Grant Number	Funding Agency Grant Number Science Technology and Research Hong Hong Kong Research Grant Council Collaborative Research Fund HKU8/CRF/09 Theme-based Research Scheme T12-705/11
Corresponding Author	Dr. Steve KW Oh, Principal Scientist, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way No. 06-01, Singapore 138668, Singapore. steve_oh@bti.a-star.edu.sg
Key Words	Soy hydrolysate; Bovine serum albumin; Differentiation; Cardiomyocyte; Human embryonic stem cells; Human induced pluripotent stem cells
Core Tip	Nutrient supplements were screened for improving cell survival during the cardiomyocyte differentiation process of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a serum-free medium based on the inhibition of p38 MAPK (SB media). Soy hydrolysate and bovine serum albumin supplementation was found to improve cell viability and therefore increased the yield of HES-3 cardiomyocytes by 2.6-fold over non-supplemented SB medium. The enhancing effect of this medium was demonstrated in an additional hESC line (H1) and Siu1-hiPSC cell line (15 and 9 fold respectively). The cardiomyocytes formed expressed canonical cardiac markers (sarcomeric ?-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like.
Publish Date	2013-07-25 08:11
Citation	Ting S, Lecina M, Chan YC, Tse HF, Reuveny S, Oh SKW. Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells. World J Stem Cells 2013; 5(3): 86-97
URL	http://www.wjgnet.com/1948-0210/full/v5/i3/86.htm
DOI	http://dx.doi.org/10.4252/wjsc.v5.i3.86

Full Article (PDF)	WJSC-5-86.pdf
Manuscript File	2977-Review.docx
Answering Reviewers	2977-Answering reviewers.docx
Copyright License Agreement	2977-Copyright assignment.PDF
Peer-review Report	2935-Peer review(s).pdf
Scientific Editor Work List	2935-Scientific editor work list.doc