BPG is committed to discovery and dissemination of knowledge
Articles Published Processes
1/12/2017 1:49:00 PM | Browse: 2033 | Download: 2354
 |
Received |
|
2016-08-17 10:47 |
|
Peer-Review Started |
|
2016-08-17 15:26 |
 |
First Decision by Editorial Office Director |
|
|
|
Return for Revision |
|
2016-10-12 10:10 |
|
Revised |
|
2016-10-25 00:23 |
|
Publication Fee Transferred |
|
|
|
Second Decision by Editor |
|
2016-11-11 16:45 |
|
Second Decision by Editor-in-Chief |
|
|
|
Final Decision by Editorial Office Director |
|
2016-11-16 13:33 |
|
Articles in Press |
|
2016-11-16 13:34 |
|
Edit the Manuscript by Language Editor |
|
|
|
Typeset the Manuscript |
|
2016-12-30 09:37 |
|
Publish the Manuscript Online |
|
2017-01-12 13:49 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
|
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease
|
| Manuscript Source |
Invited Conference Manuscripts |
| All Author List |
Tomasz Kostrzewski, Terri Cornforth, Sophie A Snow, Larissa Ouro-Gnao, Cliff Rowe, Emma M Large and David J Hughes |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Innovate UK (Technology Strategy Board) Advancing the Development and Application of Non-Animal Technologies Project: 3D cell culture model for studying Non-Alcoholic Fatty Liver Disease (NAFLD) |
131720 |
|
| Corresponding Author |
David Hughes, DPhil, CN Bio Innovations Limited, BioPark, Broadwater Rd, Welwyn Garden City AL7 3AX, United Kingdom. david.hughes@cn-bio.com |
| Key Words |
Non-alcoholic fatty liver disease; Liver disease; Three-dimensional cell culture; Organ-on-chip; Primary cell culture; Fatty liver; Hepatocytes |
| Core Tip |
We report the development of an in vitro, fully human, three-dimensional cell culture model of non-alcoholic fatty liver disease (NAFLD). The model recapitulates key features of clinical NAFLD, with primary human hepatocytes continuously loaded with fat, for up to 14 d of culture, without causing any hepa-totoxicity. Fat loading caused transcriptomic, proteomic and metabolic changes to the hepatocytes, including reduced activity of CYP3A4 and CYP2C9 enzymes. Fat loading in the model could be modulated using known anti-steatotic drugs (e.g., metformin), demonstrating the utility of the model for screening the efficacy of novel anti-steatotic compounds. |
| Publish Date |
2017-01-12 13:49 |
| Citation |
Kostrzewski T, Cornforth T, Snow SA, Ouro-Gnao L, Rowe C, Large EM, Hughes DJ. Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease. World J Gastroenterol 2017; 23(2): 204-215 |
| URL |
http://www.wjgnet.com/1007-9327/full/v23/i2/204.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v23.i2.204 |
All content on this site: Copyright © 1993-2026 Baishideng Publishing Group Inc, its licensors, and contributors. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For all open access content, the relevant licensing terms apply.
