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Articles Published Processes
1/12/2017 1:49:00 PM | Browse: 1753 | Download: 1676
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Received |
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2016-08-17 10:47 |
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Peer-Review Started |
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2016-08-17 15:26 |
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To Make the First Decision |
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2016-10-11 11:06 |
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Return for Revision |
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2016-10-12 10:10 |
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Revised |
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2016-10-25 00:23 |
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Second Decision |
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2016-11-11 16:45 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2016-11-16 13:33 |
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Articles in Press |
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2016-11-16 13:34 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2016-12-30 09:37 |
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Publish the Manuscript Online |
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2017-01-12 13:49 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease
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| Manuscript Source |
Invited Conference Manuscripts |
| All Author List |
Tomasz Kostrzewski, Terri Cornforth, Sophie A Snow, Larissa Ouro-Gnao, Cliff Rowe, Emma M Large and David J Hughes |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Innovate UK (Technology Strategy Board) Advancing the Development and Application of Non-Animal Technologies Project: 3D cell culture model for studying Non-Alcoholic Fatty Liver Disease (NAFLD) |
131720 |
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| Corresponding Author |
David Hughes, DPhil, CN Bio Innovations Limited, BioPark, Broadwater Rd, Welwyn Garden City AL7 3AX, United Kingdom. david.hughes@cn-bio.com |
| Key Words |
Non-alcoholic fatty liver disease; Liver disease; Three-dimensional cell culture; Organ-on-chip; Primary cell culture; Fatty liver; Hepatocytes |
| Core Tip |
We report the development of an in vitro, fully human, three-dimensional cell culture model of non-alcoholic fatty liver disease (NAFLD). The model recapitulates key features of clinical NAFLD, with primary human hepatocytes continuously loaded with fat, for up to 14 d of culture, without causing any hepa-totoxicity. Fat loading caused transcriptomic, proteomic and metabolic changes to the hepatocytes, including reduced activity of CYP3A4 and CYP2C9 enzymes. Fat loading in the model could be modulated using known anti-steatotic drugs (e.g., metformin), demonstrating the utility of the model for screening the efficacy of novel anti-steatotic compounds. |
| Publish Date |
2017-01-12 13:49 |
| Citation |
Kostrzewski T, Cornforth T, Snow SA, Ouro-Gnao L, Rowe C, Large EM, Hughes DJ. Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease. World J Gastroenterol 2017; 23(2): 204-215 |
| URL |
http://www.wjgnet.com/1007-9327/full/v23/i2/204.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v23.i2.204 |
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