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Publication Name World Journal of Gastroenterology
Manuscript ID 29492
Country/Territory United Kingdom
2016-08-17 10:47
Peer-Review Started
2016-08-17 15:26
To Make the First Decision
2016-10-11 11:06
Return for Revision
2016-10-12 10:10
2016-10-25 00:23
Second Decision
2016-11-11 16:45
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2016-11-16 13:33
Articles in Press
2016-11-16 13:34
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2016-12-30 09:37
Publish the Manuscript Online
2017-01-12 13:49
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Gastroenterology and Hepatology
Manuscript Type Basic Study
Article Title Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease
Manuscript Source Invited Conference Manuscripts
All Author List Tomasz Kostrzewski, Terri Cornforth, Sophie A Snow, Larissa Ouro-Gnao, Cliff Rowe, Emma M Large and David J Hughes
Funding Agency and Grant Number
Funding Agency Grant Number
Innovate UK (Technology Strategy Board) Advancing the Development and Application of Non-Animal Technologies Project: 3D cell culture model for studying Non-Alcoholic Fatty Liver Disease (NAFLD) 131720
Corresponding author David Hughes, DPhil, CN Bio Innovations Limited, BioPark, Broadwater Rd, Welwyn Garden City AL7 3AX, United Kingdom. david.hughes@cn-bio.com
Key Words Non-alcoholic fatty liver disease; Liver disease; Three-dimensional cell culture; Organ-on-chip; Primary cell culture; Fatty liver; Hepatocytes
Core Tip We report the development of an in vitro, fully human, three-dimensional cell culture model of non-alcoholic fatty liver disease (NAFLD). The model recapitulates key features of clinical NAFLD, with primary human hepatocytes continuously loaded with fat, for up to 14 d of culture, without causing any hepa-totoxicity. Fat loading caused transcriptomic, proteomic and metabolic changes to the hepatocytes, including reduced activity of CYP3A4 and CYP2C9 enzymes. Fat loading in the model could be modulated using known anti-steatotic drugs (e.g., metformin), demonstrating the utility of the model for screening the efficacy of novel anti-steatotic compounds.
Publish Date 2017-01-12 13:49
Citation Kostrzewski T, Cornforth T, Snow SA, Ouro-Gnao L, Rowe C, Large EM, Hughes DJ. Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease. World J Gastroenterol 2017; 23(2): 204-215
Url http://www.wjgnet.com/1007-9327/full/v23/i2/204.htm
DOI http://dx.doi.org/10.3748/wjg.v23.i2.204
Full Article (PDF) WJG-23-204.pdf
Full Article (Word) WJG-23-204.doc
Manuscript File 29492-Review.docx
Answering Reviewers 29492-Answering reviewers.pdf
Audio Core Tip 29492-Audio core tip.mp3
Conflict-of-Interest Disclosure Form Conflict-of-Interest_Statement_20160816235039.pdf
Copyright License Agreement 29492-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 29492-Grant application form(s).pdf
Scientific Research Process 29492-Scientific research process.pdf
Supplementary Material 29492-Supplementary material.docx
Peer-review Report 29492-Peer-review(s).pdf
Scientific Misconduct Check 29492-Scientific misconduct check.pdf
Scientific Editor Work List 29492-Scientific editor work list.pdf