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3/6/2017 3:54:00 PM | Browse: 233 | Download: 246
Publication Name World Journal of Hepatology
Manuscript ID 31507
Country of Manuscript Source Thailand
2016-11-22 11:19
Peer-Review Started
2016-11-23 15:07
To Make the First Decision
2016-12-14 08:47
Return for Revision
2016-12-15 13:50
2016-12-19 08:32
Second Decision
2017-01-04 16:10
Accepted by Journal Editor-in-Chief
2017-01-05 19:29
Accepted by Company Editor-in-Chief
2017-01-14 14:18
Articles in Press
2017-01-14 14:19
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2017-03-02 15:21
Publish the Manuscript Online
2017-03-06 15:54
ISSN 1948-5182 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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Publisher Baishideng Publishing Group Inc, 7901 Stoneridge Drive, Suite 501, Pleasanton, CA 94588, USA
Website http://www.wjgnet.com
Specialty Immunology
Manuscript Type Basic Study
Article Title Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2
Manuscript Source Invited Manuscript
All Author List Preeyanat Vongchan and Robert J Linhardt
Funding Agency and Grant Number
Funding Agency Grant Number
National Research Council of Thailand (NRCT) 2559A10402115
Correspondence To Preeyanat Vongchan, PhD, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 239 Huay Kaew Road, Muang District, Chiang Mai 50200, Thailand. preeyanat.v@cmu.ac.th
Keywords Monoclonal anti-glypican-3; Hepatocellular carcinoma; HepG2; Heparan sulfate proteoglycan; Co-immunoprecipitation
Core Tip Heparan sulfate proteoglycan (HSPG) was isolated from human liver. Preliminary results showed that it was detected by rabbit anti-glypican. Monoclonal antibody, 1E4-1D9 was raised against human liver HSPG and its specific antigen was characterized. Amino acid sequence analysis revealed that the antigen recognized by mAb 1E4-1D9 specific molecule contained no trans-membrane region. It has 15 cysteines and 11 putative glycosylation sites and 6 predicted N-glycosylation sites. The sequence matched to all PDZ domain proteins with an 85.6% match to glypican-3. Studies of co-ex-pression and co-precipitation demonstrated that mAb 1E4-1D9 could compete with anti-glypican-3. It could also react with a various tumor cell lines including solid and hematopoietic cells. The findings suggested that the antigen recognized by 1E4-1D9 was glypican-3. Moreover, findings revealed that FYCO1 co-precipitated with glypican-3 using mAb 1E4-1D9, suggesting that FYCO1 is a partner molecule of glypican-3.
Publish Date 2017-03-06 15:54
Citation Vongchan P, Linhardt RJ. Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2. World J Hepatol 2017; 9(7): 368-384
Url http://www.wjgnet.com/1948-5182/full/v9/i7/368.htm
DOI http://dx.doi.org/10.4254/wjh.v9.i7.368
Full Article (PDF) WJH-9-368.pdf
Full Article (Word) WJH-9-368.doc
Revised Manuscript 31507-Review.docx
Peer-review Report 31507-Peer-review(s).pdf
Journal Editor-in-Chief Review Report 31507-Journal editor-in-chief review report.pdf
Answering Reviewers 31507-Answering reviewers.pdf
Scientific Misconduct Check 31507-Scientific misconduct check.pdf
Scientific Editor Work List 31507-Scientific editor work list.pdf
Audio Core Tip 31507-Audio core tip.m4a
Conflict-of-Interest Disclosure Form 31507-Conflict-of-interest statement.pdf
Copyright License Agreement 31507-Copyright assignment.pdf
Data Sharing Statement 31507-Data sharing statement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 31507-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document 31507-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document 31507-Institutional review board statement.pdf