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Articles Published Processes
3/6/2017 3:54:00 PM | Browse: 1085 | Download: 1589
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Received |
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2016-11-22 11:19 |
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Peer-Review Started |
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2016-11-23 15:07 |
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To Make the First Decision |
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2016-12-14 08:47 |
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Return for Revision |
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2016-12-15 13:50 |
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Revised |
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2016-12-19 08:32 |
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Second Decision |
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2017-01-04 16:10 |
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Accepted by Journal Editor-in-Chief |
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2017-01-05 19:29 |
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Accepted by Executive Editor-in-Chief |
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2017-01-14 14:18 |
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Articles in Press |
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2017-01-14 14:19 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2017-03-02 15:21 |
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Publish the Manuscript Online |
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2017-03-06 15:54 |
| ISSN |
1948-5182 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Immunology |
| Manuscript Type |
Basic Study |
| Article Title |
Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Preeyanat Vongchan and Robert J Linhardt |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Research Council of Thailand (NRCT) |
2559A10402115 |
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| Corresponding Author |
Preeyanat Vongchan, PhD, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 239 Huay Kaew Road, Muang District, Chiang Mai 50200, Thailand. preeyanat.v@cmu.ac.th |
| Key Words |
Monoclonal anti-glypican-3; Hepatocellular carcinoma; HepG2; Heparan sulfate proteoglycan; Co-immunoprecipitation |
| Core Tip |
Heparan sulfate proteoglycan (HSPG) was isolated from human liver. Preliminary results showed that it was detected by rabbit anti-glypican. Monoclonal antibody, 1E4-1D9 was raised against human liver HSPG and its specific antigen was characterized. Amino acid sequence analysis revealed that the antigen recognized by mAb 1E4-1D9 specific molecule contained no trans-membrane region. It has 15 cysteines and 11 putative glycosylation sites and 6 predicted N-glycosylation sites. The sequence matched to all PDZ domain proteins with an 85.6% match to glypican-3. Studies of co-ex-pression and co-precipitation demonstrated that mAb 1E4-1D9 could compete with anti-glypican-3. It could also react with a various tumor cell lines including solid and hematopoietic cells. The findings suggested that the antigen recognized by 1E4-1D9 was glypican-3. Moreover, findings revealed that FYCO1 co-precipitated with glypican-3 using mAb 1E4-1D9, suggesting that FYCO1 is a partner molecule of glypican-3. |
| Publish Date |
2017-03-06 15:54 |
| Citation |
Vongchan P, Linhardt RJ. Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2. World J Hepatol 2017; 9(7): 368-384 |
| URL |
http://www.wjgnet.com/1948-5182/full/v9/i7/368.htm |
| DOI |
http://dx.doi.org/10.4254/wjh.v9.i7.368 |
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