Publication Name	World Journal of Gastroenterology
Manuscript ID	32073
Country	Canada

Received	2016-12-24 16:42
Peer-Review Started	2016-12-28 16:05
To Make the First Decision	2017-01-19 13:54
Return for Revision	2017-01-20 16:36
Revised	2017-02-14 03:40
Second Decision	2017-03-24 12:21
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2017-03-31 07:51
Articles in Press	2017-03-31 07:51
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2017-04-12 20:11
Publish the Manuscript Online	2017-04-28 10:26

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
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Website	http://www.wjgnet.com

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Droplet digital PCR for quantification of ITGA6 in a stool mRNA assay for the detection of colorectal cancers
Manuscript Source	Invited Manuscript
All Author List	Elizabeth Herring, Shigeru Kanaoka, Éric Tremblay and Jean-François Beaulieu
Funding Agency and Grant Number	Funding Agency Grant Number Canadian Institutes of Health Research PPP133373
Corresponding Author	Jean-François Beaulieu, PhD, Professor, Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec J1H5N4, Canada. jean-francois.beaulieu@usherbrooke.ca
Key Words	Colorectal cancer; Advanced adenoma; Non-invasive screening; Biomarker; mRNA
Core Tip	We investigated the use of droplet digital polymerase chain reaction (ddPCR) for detecting host mRNA markers in stools as a non-invasive test for colorectal cancer screening using ITGA6 and ITGA6A as established medium and low abundance stool biomarkers. Our side by side comparison of ddPCR and qPCR on stool samples obtained from patients with advanced adenomas and colorectal cancers and controls using duplex TaqMan reactions revealed that both methods performed equally well.
Publish Date	2017-04-28 10:26
Citation	Herring E, Kanaoka S, Tremblay E, Beaulieu JF. Droplet digital PCR for quantification of ITGA6 in a stool mRNA assay for the detection of colorectal cancers. World J Gastroenterol 2017; 23(16): 2891-2898
URL	http://www.wjgnet.com/1007-9327/full/v23/i16/2891.htm
DOI	http://dx.doi.org/10.3748/wjg.v23.i16.2891

Full Article (PDF)	WJG-23-2891.pdf
Full Article (Word)	WJG-23-2891.doc
Manuscript File	32073-Review.doc
Answering Reviewers	32073-Answering reviewers.pdf
Audio Core Tip	32073-Audio core tip.mp3
Biostatistics Review Certificate	32073-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	32073-Conflict-of-interest statement.pdf
Copyright License Agreement	32073-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	32073-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	32073-Institutional review board statement.pdf
Peer-review Report	32073-Peer-review(s).pdf
Scientific Misconduct Check	32073-Scientific misconduct check.pdf
Scientific Editor Work List	32073-Scientific editor work list.pdf