Publication Name	World Journal of Gastroenterology
Manuscript ID	33280
Country	France

Received	2017-02-08 11:03
Peer-Review Started	2017-02-08 15:21
To Make the First Decision	2017-04-05 17:18
Return for Revision	2017-04-06 09:40
Revised	2017-05-06 03:36
Second Decision	2017-07-06 07:14
Accepted by Journal Editor-in-Chief	2017-07-06 07:19
Accepted by Executive Editor-in-Chief	2017-07-12 05:59
Articles in Press	2017-07-12 05:59
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2017-07-22 12:34
Publish the Manuscript Online	2017-07-28 08:16

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article, which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Involvement of CRF2 signaling in enterocyte differentiation
Manuscript Source	Invited Manuscript
All Author List	Benjamin Ducarouge, Marjolaine Pelissier-Rota, Rebecca Powell, Alain Buisson, Bruno Bonaz and Muriel Jacquier-Sarlin
Funding Agency and Grant Number	Funding Agency Grant Number Association pour la Recherche sur le Cancer, Ligue Nationale contre le Cancer GEFLUC Association pour la Recherche sur le Cancer, Ligue Nationale contre le Cancer ESPOIR
Corresponding Author	Muriel Jacquier-Sarlin, PhD, Assistant Professor, Equipes des “Neuropathologies et dysfonctions synaptiques” et du “Stress et des interactions neurodigestives”, Université Grenoble Alpes and Grenoble Institut des Neurosciences-Inserm U1216, Site Santé BP 170 La Tronche, Grenoble F-38000, France. muriel.jacquier-sarlin@univ-grenoble-alpes.fr
Key Words	Urocortin3; Colon carcinoma cell lines; Intercellular adhesion complexes; Cell permeability; Epithelial cell differentiation; Corticotrophin releasing factor 2
Core Tip	Stress is recognized to participate in the development and/or aggravation of gastrointestinal (GI) disorders mainly by altering intestinal epithelial functions. This occurs partly through the deregulation of neuromediator secretion, but their activity on enterocyte differentiation remains not totally under­stood. We found that expression of corticotropin releasing factor receptor 2 (CRF2), a stress ligand receptor is inversely correlated to the differentiation status of colon cell lines. We thus investigated the effect of CRF2 signaling on intestinal epithelial differentiation using Ucn3 agonist treatments applied to different cancer cell lines that mimic this process. We identified that CRF2 activation affect both the early steps of differentiation and an established differentiated state, by down-regulating transcription factors such as krüppel-like factor 4. This effect is correlated with alterations of epithelial permeability and cellular adhesion junctions. Our results argue that CFR2-induced alterations of enterocyte differentiation may contribute to stress-mediated barrier dysfunction and the development of GI disorders.
Publish Date	2017-07-28 08:16
Citation	Ducarouge B, Pelissier-Rota M, Powell R, Buisson A, Bonaz B, Jacquier-Sarlin M. Involvement of CRF2 signaling in enterocyte differentiation. World J Gastroenterol 2017; 23(28): 5127-5145
URL	http://www.wjgnet.com/1007-9327/full/v23/i28/5127.htm
DOI	http://dx.doi.org/10.3748/wjg.v23.i28.5127

Full Article (PDF)	WJG-23-5127.pdf
Full Article (Word)	WJG-23-5127.doc
Manuscript File	33280-Review.docx
Answering Reviewers	33280-Answering reviewers.pdf
Audio Core Tip	33280-Audio core tip.wav
Biostatistics Review Certificate	33280-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	33280-Conflict-of-interest statement.pdf
Copyright License Agreement	33280_Copyright assignment Ducarouge et al WJG.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	33280-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	33280-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	33280-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	33280-Language certificate.pdf
Peer-review Report	33280-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	33280-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	33280-Scientific misconduct check.pdf
Scientific Editor Work List	33280-Scientific editor work list.pdf