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Articles Published Processes
5/19/2017 7:33:03 AM | Browse: 1243 | Download: 2712
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Received |
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2017-02-07 10:57 |
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Peer-Review Started |
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2017-02-07 13:49 |
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To Make the First Decision |
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2017-03-06 18:16 |
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Return for Revision |
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2017-03-07 16:02 |
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Revised |
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2017-04-03 11:41 |
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Second Decision |
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2017-04-24 17:54 |
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Accepted by Journal Editor-in-Chief |
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2017-04-25 07:29 |
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Accepted by Executive Editor-in-Chief |
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2017-05-05 16:41 |
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Articles in Press |
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2017-05-05 16:41 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2017-05-15 06:06 |
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Publish the Manuscript Online |
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2017-05-19 07:33 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Next-generation sequencing traces human induced pluripotent stem cell lines clonally generated from heterogeneous cancer tissue
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Tetsuya Ishikawa |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| JSPS KAKENHI |
16K07135 |
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| Corresponding Author |
Tetsuya Ishikawa, PhD (DMSc), Laboratory Head, Central Animal Division, Fundamental Innovative Oncology Core Center, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan. humanipscells@gmail.com |
| Key Words |
Colon cancer; Next-generation sequencing; Single-nucleotide variant; Genotype; Heterogeneous cancer tissue; Cancer associated fibroblast; Pre-cancer cells; Induced pluripotent stem cell; Single cell; Clonal evolution |
| Core Tip |
Ten induced pluripotent stem cell (iPSC) lines were clonally generated from heterogeneous colon cancer tissues and analyzed with next-generation sequencing. Non-synonymous single-nucleotide variants (SNVs) of the iPSC lines were not identical to the genotypes of the cancer tissues. The SNVs were de novo or pre-existing mutations that originated from a minor population within the cancer tissue. Meanwhile, the genotypes of the iPSC lines were not mutated genotypes of the cancer tissues, suggesting that the starting cells for the iPSC lines were not mature cancer cells. Thus, the genotypes of iPSC lines can be used to trace the genomic origins of single cells within heterogeneous cancer tissue. |
| Publish Date |
2017-05-19 07:33 |
| Citation |
Ishikawa T. Next-generation sequencing traces human induced pluripotent stem cell lines clonally generated from heterogeneous cancer tissue. World J Stem Cells 2017; 9(5): 77-88 |
| URL |
URL: http://www.wjgnet.com/1948-0210/full/v9/i5/77.htm |
| DOI |
http://dx.doi.org/10.4252/wjsc.v9.i5.77 |
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