Publication Name	World Journal of Gastroenterology
Manuscript ID	3352
Country	China

Received	2013-04-24 10:02
Peer-Review Started	2013-04-24 14:22
First Decision by Editorial Office Director
Return for Revision	2013-07-01 13:31
Revised
Publication Fee Transferred
Second Decision by Editor	2013-09-29 12:32
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2013-09-29 22:15
Articles in Press
Edit the Manuscript by Language Editor	2013-12-20 13:40
Typeset the Manuscript	2014-01-10 14:54
Publish the Manuscript Online	2014-01-26 20:08

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Radiology, Nuclear Medicine & Medical Imaging
Manuscript Type	Autobiography
Article Title	Novel esophageal squamous cell carcinoma bone metastatic clone isolated by scintigraphy, X ray and micro PET/CT
Manuscript Source	Invited Manuscript
All Author List	Bi-Zeng Zhao, Jie Cao, Jin-Chen Shao, Yan-Bing Sun, Li-Min Fan, Chun-Yu Wu, Sheng Liang, Bao-Feng Guo, Guang Yang, Wen-Hui Xie, Qing-Cheng Yang and Shun-Fang Yang
Funding Agency and Grant Number	Funding Agency Grant Number Shanghai Science and Technology fundamental research 08140902202 Shanghai Science and Technology fundamental research 09140901500 (to Yang SF) National Natural Science Foundation of China 30973017 (to Yang QC)
Corresponding Author	Yang Shun-Fang, Associate Professor, Department of Nuclear Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China. yzyg@sh163.net
Key Words	Esophageal squamous cell carcinoma; Cell line; Bone metastasis; Molecular imaging; Real-time polymerase chain reaction
Core Tip	We established a novel esophageal squamous cell carcinoma cell line from a surgically resected human specimen and its clone with mixed bone metastasis potency by molecular imaging including conventional radiography, bone scintigraphy, and micro-positron emission tomography/computed tomography after intracardiac inoculation of the cells into nude mice. The process was repeated in vivo and in vitro for four cycles to obtain a bone metastasis clone CEK-Sq-1BM. Some gene expression difference was compared with the primary cells and their clone by real-time reverse transcriptase polymerase chain reaction. This work may be helpful for research in bone metastases.
Publish Date	2014-01-26 20:08
Citation	Zhao BZ, Cao J, Shao JC, Sun YB, Fan LM, Wu CY, Liang S, Guo BF, Yang G, Xie WH, Yang QC, Yang SF. Novel esophageal squamous cell carcinoma bone metastatic clone isolated by scintigraphy, X ray and micro PET/CT. World J Gastroenterol 2014; 20(4): 1030-1037
URL	http://www.wjgnet.com/1007-9327/full/v20/i4/1030.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i4.1030

Full Article (PDF)	WJG-20-1030.pdf
Full Article (Word)	WJG-20-1030.doc
Manuscript File	3352-Review.doc
Answering Reviewers	3352-Answering reviewers.pdf
Copyright License Agreement	3352-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	3352-Language certificate.pdf
Peer-review Report	3352-Peer review(s).pdf
Scientific Editor Work List	3352-Scientific editor work list.doc