| Category |
Radiology, Nuclear Medicine & Medical Imaging |
| Manuscript Type |
Autobiography |
| Article Title |
Novel esophageal squamous cell carcinoma bone metastatic clone isolated by scintigraphy, X ray and micro PET/CT
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Bi-Zeng Zhao, Jie Cao, Jin-Chen Shao, Yan-Bing Sun, Li-Min Fan, Chun-Yu Wu, Sheng Liang, Bao-Feng Guo, Guang Yang, Wen-Hui Xie, Qing-Cheng Yang and Shun-Fang Yang |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Shanghai Science and Technology fundamental research |
08140902202 |
| Shanghai Science and Technology fundamental research |
09140901500 (to Yang SF) |
| National Natural Science Foundation of China |
30973017 (to Yang QC) |
|
| Corresponding Author |
Yang Shun-Fang, Associate Professor, Department of Nuclear Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China. yzyg@sh163.net
|
| Key Words |
Esophageal squamous cell carcinoma; Cell line; Bone metastasis; Molecular imaging; Real-time polymerase chain reaction |
| Core Tip |
We established a novel esophageal squamous cell carcinoma cell line from a surgically resected human specimen and its clone with mixed bone metastasis potency by molecular imaging including conventional radiography, bone scintigraphy, and micro-positron emission tomography/computed tomography after intracardiac inoculation of the cells into nude mice. The process was repeated in vivo and in vitro for four cycles to obtain a bone metastasis clone CEK-Sq-1BM. Some gene expression difference was compared with the primary cells and their clone by real-time reverse transcriptase polymerase chain reaction. This work may be helpful for research in bone metastases.
|
| Publish Date |
2014-01-26 20:08 |
| Citation |
Zhao BZ, Cao J, Shao JC, Sun YB, Fan LM, Wu CY, Liang S, Guo BF, Yang G, Xie WH, Yang QC, Yang SF. Novel esophageal squamous cell carcinoma bone metastatic clone isolated by scintigraphy, X ray and micro PET/CT. World J Gastroenterol 2014; 20(4): 1030-1037 |
| URL |
http://www.wjgnet.com/1007-9327/full/v20/i4/1030.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v20.i4.1030 |
