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Articles Published Processes
10/21/2017 7:10:37 AM | Browse: 1218 | Download: 2020
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Received |
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2017-06-28 01:42 |
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Peer-Review Started |
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2017-06-28 22:59 |
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To Make the First Decision |
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2017-08-15 03:41 |
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Return for Revision |
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2017-08-16 09:27 |
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Revised |
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2017-09-15 09:14 |
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Second Decision |
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2017-09-25 06:29 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2017-09-26 01:49 |
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Articles in Press |
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2017-09-26 01:49 |
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Publication Fee Transferred |
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2017-09-27 07:38 |
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Edit the Manuscript by Language Editor |
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2017-10-02 23:20 |
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Typeset the Manuscript |
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2017-10-15 07:42 |
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Publish the Manuscript Online |
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2017-10-21 07:10 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Research & Experimental Medicine |
| Manuscript Type |
Basic Study |
| Article Title |
Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Susana Olmedillas-López, Dennis César Lévano-Linares, Carmen Laura Aúz Alexandre, Luz Vega-Clemente, Edurne León Sánchez, Alejandro Villagrasa, Jaime Ruíz-Tovar, Mariano García-Arranz and Damian Garcia-Olmo |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| “Fondo de Investigaciones Sanitarias (FIS)-FEDER”, Ministry of Health, Spain |
PI13/01924 |
| RETIC Program of ISCIII-FEDER |
RD12/0019/0035 |
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| Corresponding Author |
Susana Olmedillas-López, PhD, Foundation Health Research Institute-Fundación Jiménez Díaz University Hospital, Avda. Reyes Católicos 2, Madrid 28040, Spain. susana.olmedillas@fjd.es |
| Key Words |
Droplet digital PCR; KRAS; Stool; Formalin-fixed paraffin-embedded; Pyrosequencing; Colorectal cancer |
| Core Tip |
The potential of droplet digital PCR (ddPCR) to detect KRAS G12D mutation in stool DNA from colorectal cancer (CRC) patients was examined as a proof-of-concept for the applicability of this technology to study DNA biomarkers in stool-derived DNA. It was shown that KRAS G12D detection in stool-derived DNA from CRC patients by ddPCR is feasible and provides comparable results to the analysis of formalin-fixed paraffin-embedded tissue by pyrosequencing. These results suggest that analysis of KRAS mutations and other molecular biomarkers in stool by ddPCR could represent a complementary non-invasive approach to standard screening tests for CRC. |
| Publish Date |
2017-10-21 07:10 |
| Citation |
Olmedillas-López S, Lévano-Linares DC, Aúz Alexandre CL, Vega-Clemente L, León Sánchez E, Villagrasa A, Ruíz-Tovar J, García-Arranz M, García-Olmo D. Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR. World J Gastroenterol 2017; 23(39): 7087-7097 |
| URL |
http://www.wjgnet.com/1007-9327/full/v23/i39/7087.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v23.i39.7087 |
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