Publication Name	World Journal of Stem Cells
Manuscript ID	35654
Country	Spain

Received	2017-08-14 00:50
Peer-Review Started	2017-08-14 06:22
To Make the First Decision	2017-09-25 00:42
Return for Revision	2017-09-25 08:39
Revised	2017-10-26 17:34
Second Decision	2017-11-20 02:29
Accepted by Journal Editor-in-Chief	2017-11-20 05:58
Accepted by Company Editor-in-Chief	2017-11-28 01:43
Articles in Press	2017-11-28 01:43
Publication Fee Transferred
Edit the Manuscript by Language Editor	2018-01-16 22:32
Typeset the Manuscript	2018-01-25 03:12
Publish the Manuscript Online	2018-01-25 07:28

ISSN	1948-0210 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Rat model of anal sphincter injury and two approaches for stem cell administration
Manuscript Source	Invited Manuscript
All Author List	Jacobo Trebol, Tihomir Georgiev-Hristov, Luz Vega-Clemente, Ignacio Garcia-Gomez, Ana Carabias-Orgaz, Mariano Garcia-Arranz and Damian Garcia-Olmo
ORCID	Author(s) ORCID Number Jacobo Trebol http://orcid.org/0000-0002-6579-533X Tihomir Georgiev-Hristov http://orcid.org/0000-0002-4428-6413 Luz Vega-Clemente http://orcid.org/0000-0002-8558-1937 Ignacio Garcia-Gomez http://orcid.org/0000-0002-3580-0986 Ana Carabias-Orgaz http://orcid.org/0000-0002-6579-533X Mariano Garcia-Arranz http://orcid.org/0000-0002-6266-9055 Damian Garcia-Olmo http://orcid.org/0000-0002-9369-2338
Funding Agency and Grant Number	Funding Agency Grant Number Spanish Ministry of Health and Consumer Affairs FIS project PI060305
Corresponding Author	Jacobo Trebol, PhD, Surgeon, Surgical Oncologist, Department of General and Digestive Tract Surgery, University Hospital “La Paz”, Paseo de la Castellana, nº 261., Madrid 28046, Spain. jacobo.trebol@salud.madrid.org
Key Words	Fecal incontinence; Experimental rat model; Anal sphincter; Cell implantation; Cell therapy; Stem cells; Mesenchymal stem cell
Core Tip	Fecal incontinence is very frequent and associates severe consequences for patients. Surgical treatment outcomes are not as good as they should be, mainly in the long term. Stem cells could improve these results as demonstrated in other clinical settings. We report a simple rat model for experimental anal sphincters injury, a surgical section, and characterize it from a morphological (conventional histology and immunofluorescence) and functional (anal manometry) point of view. Then, we describe two ways of adipose derived stem cell administration to the injured area, injection and biosutures, and demonstrate stem cells survival during at least seven days, as well as their safety.
Publish Date	2018-01-25 07:28
Citation	Trébol J, Georgiev-Hristov T, Vega-Clemente L, García-Gómez I, Carabias-Orgaz A, García-Arranz M, García-Olmo D. Rat model of anal sphincter injury and two ways for stem cells administration. World J Stem Cells 2018; 10(1): 1-14
URL	http://www.wjgnet.com/1948-0210/full/v10/i1/1.htm
DOI	http://dx.doi.org/10.4252/wjsc.v10.i1.1

Full Article (PDF)	WJSC-10-1.pdf
Full Article (Word)	WJSC-10-1.doc
Manuscript File	35654-Review.doc
Answering Reviewers	35654-Answering reviewers.pdf
Audio Core Tip	35654-Audio core tip.wav
Biostatistics Review Certificate	35654-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	35654-Conflict-of-interest statement.pdf
Copyright License Agreement	35654-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	35654-Approved-Grant-Application-Form(s)-or-Funding-Agency-Copy-of-any-Approval-Document(s)-revision.pdf
Institutional Animal Care and Use Committee Approval Form or Document	35654-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	35654-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	35654-Language certificate.pdf
Supplementary Material	35654-Supplementary-Material-revision_3337.pdf
Peer-review Report	35654-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	35654-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	35654-Scientific misconduct check.pdf
Scientific Editor Work List	35654-Scientific editor work list.pdf