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Articles Published Processes
3/26/2018 8:48:04 AM | Browse: 1025 | Download: 1503
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Received |
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2017-12-01 03:40 |
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Peer-Review Started |
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2017-12-01 14:19 |
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To Make the First Decision |
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2017-12-20 07:42 |
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Return for Revision |
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2017-12-21 12:34 |
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Revised |
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2018-01-04 03:52 |
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Second Decision |
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2018-01-18 09:48 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2018-01-18 15:09 |
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Articles in Press |
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2018-01-18 15:09 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2018-02-08 07:38 |
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Typeset the Manuscript |
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2018-03-15 09:14 |
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Publish the Manuscript Online |
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2018-03-26 08:48 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Qi Wang, Yue Li, Shun-Ai Liu, Wen Xie and Jun Cheng |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Beijing Municipal Administration of Hospitals’ Youth Program |
QML20161801 |
| Beijing Municipal Administration of Hospital Programs |
DFL2015170 |
| Beijing Municipal Administration of Hospital Programs |
ZYLX201402 |
| National Natural Science Foundation of China |
81500439 |
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| Corresponding Author |
Jun Cheng, MD, PhD, Professor, Center of Liver Diseases, Beijing Ditan Hospital, Capital Medical University, No. 8 East Jingshun Street, Chaoyang District, Beijing 100015, China. chengj0817@ccmu.edu.cn |
| Key Words |
Hepatitis C virus; JFH1; Adaptive mutation; RNA replication; Virion release; Lipid droplet localization |
| Core Tip |
In this study, we explored hepatitis C virus (HCV) adaptive mutations or combinations thereof responsible for enhanced viral production and investigated the underlying mechanisms. We generated infectious HCV particles with a robust titer of 1.61 × 106 focus-forming units (FFUs)/mL, and confirmed that the adaptive mutations could enhance viral replication and release. The results were established at the levels of infectious particle titers, HCV RNA, protein expression, virus release, lipid droplet, and NS5A co-localization, and further the ratio between p56 and p58 phosphoforms of NS5A. |
| Publish Date |
2018-03-26 08:48 |
| Citation |
Wang Q, Li Y, Liu SA, Xie W, Cheng J. Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release. World J Gastroenterol 2018; 24(12): 1299-1311 |
| URL |
http://www.wjgnet.com/1007-9327/full/v24/i12/1299.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v24.i12.1299 |
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