ISSN |
1007-9327 (print) and 2219-2840 (online) |
Open Access |
This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
Copyright |
© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. |
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Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
Website |
http://www.wjgnet.com |
Category |
Gastroenterology & Hepatology |
Manuscript Type |
Basic Study |
Article Title |
Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines
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Manuscript Source |
Invited Manuscript |
All Author List |
Michael Gock, Christina S Mullins, Carina Bergner, Friedrich Prall, Robert Ramer, Anja Goeder, Oliver H Krämer, Falko Lange, Bernd J Krause, Ernst Klar and Michael Linnebacher |
ORCID |
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Funding Agency and Grant Number |
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Corresponding Author |
Michael Linnebacher, PhD, Postdoc, Research Scientist, Section of Molecular Oncology and Immunotherapy, University Medical Center, Schillingallee 35, Rostock 18055, Germany. michael.linnebacher@med.uni-rostock.de |
Key Words |
Patient-derived tumor model; Rectal cancer; 18F-fluorodesoxyglucose; 18F-fluorothymidine; FOLFOX; FOLFIRI; Personalized medicine |
Core Tip |
Ultra-low passage and in-depth characterized tumor models are highly desirable for basic research and assessment of individual response prediction to current or novel therapy regimens. Here, for the first time, we describe three patient-derived rectal cancer cell lines established either directly from patient’s tumor samples or after xenografting. These tumor models were characterized according to phenotype, molecular-, as well as growth and morphological features and sensitivity to chemotherapeutic drugs and radiation, including radiochemotherapy. In addition, glucose metabolism was assessed with 18F-fluorodeoxyglucose and proliferation with 18F-fluorothymidine. These cell lines provide excellent tools for basic and translational research of rectal cancers’ biological characteristics. |
Publish Date |
2018-11-21 04:08 |
Citation |
Gock M, Mullins CS, Bergner C, Prall F, Ramer R, Göder A, Krämer OH, Lange F, Krause BJ, Klar E, Linnebacher M. Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines. World J Gastroenterol 2018; 24(43): 4880-4892 |
URL |
http://www.wjgnet.com/1007-9327/full/v24/i43/4880.htm |
DOI |
http://dx.doi.org/10.3748/wjg.v24.i43.4880 |