| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Physiology |
| Manuscript Type |
Basic Study |
| Article Title |
Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Shih-Chi Chao, Gwo-Jang Wu, Shu-Fu Huang, Nian-Tzy Dai, Hsu-Kai Huang, Shiao-Pieng Lee, Mei-Fang Chou, Yi-Ting Tsai and Shih-Hurng Loh |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Ministry of Science and Technology Grants of Taiwan |
MOST 106-2320-B-016-003-MY2 (to Loh SH) |
| Ministry of Science and Technology Grants of Taiwan |
MOST 106-2314-B-016 -037-MY3 (to Tsai YT) |
| National Defense Medical Center Grants of Taiwan |
MAB-106-033 (to Loh SH) |
| National Defense Medical Center Grants of Taiwan |
MAB-105-043 (to Dai NZ) |
| National Defense Medical Center Grants of Taiwan |
MAB-106-034 (to Dai NZ) |
| Teh-Tzer Study Group for Human Medical Research Foundation of Taiwan |
A1061037 (to Loh SH) |
| Teh-Tzer Study Group for Human Medical Research Foundation of Taiwan |
A1061054 (to Loh SH) |
|
| Corresponding Author |
Shih-Hurng Loh, DPhil, Director, Director, Full Professor, Department of Pharmacology, and Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, No. 161, Sec. 6, Minquan E. Rd., Taipei 11490, Taiwan. shloh@ndmctsgh.edu.tw |
| Key Words |
microspectrofluorimetry; human induced pluripotent stem cells; Na+/H+ exchanger; Na+/HCO3- cotransporter; Cl-/OH- exchanger; Cl-/HCO3- exchanger; V-ATPase; intracellular buffering power; intracellular pH; BCECF |
| Core Tip |
For the first time, we established a model of the intracellular pH (pHi) regulation mechanism in human induced pluripotent stem cells (hiPSCs). The steady-state pHi value of hiPSCs was 7.50-7.68, which greater than that of normal adult cells. The Na+-H+ exchanger, the Na+-HCO3- cotransporter and vacuolar-ATPase were the main acid extruders, while the Cl--HCO3- anion exchanger and the Cl--OH- exchanger were the main acid loaders. Moreover, the pHi and acid-extruding mechanism were decreased during the loss of pluripotency in hiPSCs. pHi regulators represent an attractive target for differentiation efficiency or culture quality. |
| Publish Date |
2018-12-25 02:53 |
| Citation |
Chao SC, Wu GJ, Huang SF, Dai NT, Huang HK, Chou MF, Tsai YT, Lee SP, Loh SH. Functional and molecular mechanism of intracellular ph regulation in human inducible pluripotent stem cells. World J Stem Cells 2018; 10(12): 196-211 |
| URL |
https://www.wjgnet.com/1948-0210/full/v10/i12/196.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v10.i12.196 |
