Publication Name	World Journal of Gastroenterology
Manuscript ID	4742
Country	Germany

Received	2013-07-20 09:21
Peer-Review Started	2013-07-22 19:33
To Make the First Decision	2013-09-02 16:26
Return for Revision	2013-09-03 21:33
Revised	2013-09-10 22:29
Second Decision	2013-09-17 09:44
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2013-09-17 11:25
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-11-21 09:52
Publish the Manuscript Online	2013-12-11 16:13

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Virology
Manuscript Type	Autobiography
Article Title	Identification and characterization of a novel bipartite nuclear localization signal in the hepatitis B virus polymerase
Manuscript Source	Invited Manuscript
All Author List	Joachim Lupberger, Stephanie Schaedler, Alexander Peiran and Eberhard Hildt
Funding Agency and Grant Number	Funding Agency Grant Number DZIF to Hildt E
Corresponding Author	Eberhard Hildt, Professor, Division of Virology, Paul-Ehrlich-Institute, Paul-Ehrlich-Str. 51-59, D-63225 Langen, Germany. eberhard.hildt@pei.de
Key Words	Hepatitis B virus; Nuclear localization signal; Casein kinase Ⅱ; Trafficking; Replication
Core Tip	The mechanism mediating import of the hepatitis B virus (HBV) genome into the nucleus is still not fully understood. We describe the identification and characterization of a bipartite nuclear localization signal (NLS) in the HBV polymerase that harbours a phosphorylation site for casein kinase Ⅱ (CKⅡ). Integrity of the phosphorylation site is crucial for the functionality of the NLS. Moreover, inhibition of CKⅡ prevents karyopherin-?2 from binding to the polymerase and thereby the import of the polymerase is impaired. Analysing the viral life cycle we observed that inhibition of CKⅡ blocks the import of the genome into the nucleus resulting in impaired cccDNA formation and so the establishment of the viral infection is prevented.
Publish Date	2013-12-11 16:13
Citation	Lupberger J, Schaedler S, Peiran A, Hildt E. Identification and characterization of a novel bipartite nuclear localization signal in the hepatitis B virus polymerase. World J Gastroenterol 2013; 19(44): 8000-8010
URL	http://www.wjgnet.com/1007-9327/full/v19/i44/8000.htm
DOI	http://dx.doi.org/10.3748/wjg.v19.i44.8000

Full Article (PDF)	WJG-19-8000.pdf
Manuscript File	4742-Review.doc
Answering Reviewers	4742-Answering reviewers.pdf
Copyright License Agreement	4742-Copyright assignment.doc
Peer-review Report	4742-Peer review(s).pdf
Scientific Editor Work List	4742-Scientific editor work list.doc